##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BbrPI BfrI BglII BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DrdI Eam1105I Eco81I EcoRI EcoRV
FspI HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 894 GACGTC GACGT^C
AcsI 241 AAATTT [GA]^AATT[CT]
AgeI 3854 ACCGGT A^CCGGT
ApoI 241 AAATTT [GA]^AATT[CT]
AvrII 3035 CCTAGG C^CTAGG
BbsI 3834 GAAGAC GAAGAC(2/6)
BcgI 3403 GCAATAAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3578 GTATCC GTATCC(6/5)
BclI 3924 TGATCA T^GATCA
BlnI 3035 CCTAGG C^CTAGG
BlpI 659 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 659 GCTGAGC GC^T[ACGT]AGC
BpuAI 3834 GAAGAC GAAGAC(2/6)
BspLU11I 1114 ACATGT A^CATGT
BsrDI 746 CATTGC GCAATG(2/0)
BssSI 3346 CTCGTG CACGAG(-5/-1)
BstEII 1679 GGTGACC G^GT[ACGT]ACC
BstPI 1679 GGTGACC G^GT[ACGT]ACC
CelII 659 GCTGAGC GC^T[ACGT]AGC
DraIII 3765 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1679 GGTGACC G^GT[ACGT]ACC
FbaI 3924 TGATCA T^GATCA
FseI 42 GGCCGGCC GGCCGG^CC
HincII 390 GTTGAC GT[CT]^[GA]AC
HindII 390 GTTGAC GT[CT]^[GA]AC
HindIII 3561 AAGCTT A^AGCTT
KspI 13 CCGCGG CCGC^GG
PaeR7I 31 CTCGAG C^TCGAG
PinAI 3854 ACCGGT A^CCGGT
SacI 1443 GAGCTC GAGCT^C
SacII 13 CCGCGG CCGC^GG
SapI 173 GAAGAGC GCTCTTC(1/4)
SbfI 3416 CCTGCAGG CCTGCA^GG
SexAI 3212 ACCTGGT A^CC[AT]GGT
SfiI 3728 GGCCCTCTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 31 CTCGAG C^T[CT][GA]AG
Sse8387I 3416 CCTGCAGG CCTGCA^GG
XhoI 31 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 1114 ACATGT A^C[GA][CT]GT
2677 ACGTGT
Aor51HI 833 AGCGCT AGC^GCT
1073 AGCGCT
Asp700I 1289 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2642 GAAAGCCTTC
AspHI 1443 GAGCTC G[AT]GC[AT]^C
3396 GAGCAC
BbeI 1389 GGCGCC GGCGC^C
2403 GGCGCC
BsiHKAI 1443 GAGCTC G[AT]GC[AT]^C
3396 GAGCAC
BstAPI 2763 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3764 GCACAGGGTGC
Eco47III 833 AGCGCT AGC^GCT
1073 AGCGCT
EcoNI 2334 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3353 CCTGCTGGAGG
EcoT22I 1532 ATGCAT ATGCA^T
3641 ATGCAT
KasI 1389 GGCGCC G^GCGCC
2403 GGCGCC
NarI 1389 GGCGCC GG^CGCC
2403 GGCGCC
NcoI 2773 CCATGG C^CATGG
3070 CCATGG
NsiI 1532 ATGCAT ATGCA^T
3641 ATGCAT
NspI 413 GCATGT [GA]CATG^[CT]
1114 ACATGT
PflMI 2280 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2482 CCAGTGTGTGG
SfoI 1389 GGCGCC GGC^GCC
2403 GGCGCC
Van91I 2280 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2482 CCAGTGTGTGG
XmnI 1289 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2642 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI