##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BlnI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
Bst1107I BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I FauI FbaI FseI
FspI HaeII KasI KspI MluI
MroI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1050 GGTACC G^GTACC
AciI 1868 GCGG CCGC(-3/-1)
AcyI 2550 GGCGTC G[GA]^CG[CT]C
AhdI 2380 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1438 GGATC GGATC(4/5)
AlwNI 2536 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 646 CCTGAGG CC^T[ACGT]AGG
AseI 3716 ATTAAT AT^TAAT
AsnI 3716 ATTAAT AT^TAAT
Asp718I 1050 GGTACC G^GTACC
AspEI 2380 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 645 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2807 AGATCT A^GATCT
BlpI 2215 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2215 GCTGAGC GC^T[ACGT]AGC
BsaHI 2550 GGCGTC G[GA]^CG[CT]C
BsaI 3490 GGTCTC GGTCTC(1/5)
BsmI 3242 GAATGC GAATGC(1/-1)
Bsp1407I 997 TGTACA T^GTACA
BsrGI 997 TGTACA T^GTACA
BssSI 2896 CTCGTG CACGAG(-5/-1)
BstAPI 733 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1994 TTCGAA TT^CGAA
BstEII 2827 GGTGACC G^GT[ACGT]ACC
BstPI 2827 GGTGACC G^GT[ACGT]ACC
Bsu36I 646 CCTGAGG CC^T[ACGT]AGG
CelII 2215 GCTGAGC GC^T[ACGT]AGC
CfoI 1788 GCGC GCG^C
DraII 877 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 743 CCATGG C^C[GA][CT]GG
Eam1105I 2380 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 646 CCTGAGG CC^T[ACGT]AGG
EcoO109I 877 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2827 GGTGACC G^GT[ACGT]ACC
EcoRV 2708 GATATC GAT^ATC
HapII 3233 CCGG C^CGG
HgaI 2551 GCGTC GACGC(5/10)
HhaI 1788 GCGC GCG^C
Hin1I 2550 GGCGTC G[GA]^CG[CT]C
HinP1I 1788 GCGC G^CGC
HindIII 1724 AAGCTT A^AGCTT
HpaI 1276 GTTAAC GTT^AAC
HpaII 3233 CCGG C^CGG
KpnI 1050 GGTACC GGTAC^C
MfeI 1313 CAATTG C^AATTG
MspI 3233 CCGG C^CGG
MunI 1313 CAATTG C^AATTG
NcoI 743 CCATGG C^CATGG
NdeI 2954 CATATG CA^TATG
NspV 1994 TTCGAA TT^CGAA
PshBI 3716 ATTAAT AT^TAAT
SacI 3565 GAGCTC GAGCT^C
SapI 1930 GCTCTTC GCTCTTC(1/4)
SexAI 2930 ACCAGGT A^CC[AT]GGT
SfuI 1994 TTCGAA TT^CGAA
SmaI 3232 CCCGGG CCC^GGG
SmlI 686 CTCAAG C^T[CT][GA]AG
SspBI 997 TGTACA T^GTACA
XbaI 24 TCTAGA T^CTAGA
XcmI 3176 CCAGTTACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3232 CCCGGG C^CCGGG
XmaI 3232 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 94 CCCGAG C^[CT]CG[GA]G
3232 CCCGGG
AvaII 986 GGACC G^G[AT]CC
3208 GGACC
BanII 9 GAGCCC G[GA]GC[CT]^C
3565 GAGCTC
BciVI 3575 GGATAC GTATCC(6/5)
1941 GTATCC
BcnI 3232 CCCGG CC^[GC]GG
3233 CCGGG
BsaAI 667 TACGTG [CT]AC^GT[GA]
2671 TACGTG
BsaBI 1137 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3576 GATACCCATC
BsoBI 94 CCCGAG C^[CT]CG[GA]G
3232 CCCGGG
BspLU11I 2053 ACATGT A^CATGT
2348 ACATGT
BspMI 276 ACCTGC ACCTGC(4/8)
1236 GCAGGT
BsrDI 500 CATTGC GCAATG(2/0)
440 GCAATG
BstXI 939 CCAGAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3310 CCACCTGACTGG
BstYI 1438 GGATCT [GA]^GATC[CT]
2807 AGATCT
DraI 1443 TTTAAA TTT^AAA
3363 TTTAAA
EarI 1931 CTCTTC CTCTTC(1/4)
370 GAAGAG
EcoNI 957 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3250 CCTGCTGGAGG
EcoRI 658 GAATTC G^AATTC
1997 GAATTC
EcoT22I 497 ATGCAT ATGCA^T
1684 ATGCAT
HincII 118 GTTGAC GT[CT]^[GA]AC
1276 GTTAAC
HindII 118 GTTGAC GT[CT]^[GA]AC
1276 GTTAAC
Ksp632I 1931 CTCTTC CTCTTC(1/4)
370 GAAGAG
MamI 1137 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3576 GATACCCATC
MflI 1438 GGATCT [GA]^GATC[CT]
2807 AGATCT
NciI 3232 CCCGG CC^[GC]GG
3233 CCGGG
NsiI 497 ATGCAT ATGCA^T
1684 ATGCAT
TaqI 1995 TCGA T^CGA
3619 TCGA
TthHB8I 1995 TCGA T^CGA
3619 TCGA
XhoII 1438 GGATCT [GA]^GATC[CT]
2807 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AspHI BalI BanI BbsI BbvCI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I