##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BglI BglII BlnI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NcoI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1493 CGCG CG^CG
AclI 983 AACGTT AA^CGTT
AcyI 1196 GGCGTC G[GA]^CG[CT]C
AflII 793 CTTAAG C^TTAAG
Asp700I 1327 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfrI 793 CTTAAG C^TTAAG
BlpI 415 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC
BsaAI 2774 TACGTG [CT]AC^GT[GA]
BsaBI 1919 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1196 GGCGTC G[GA]^CG[CT]C
BsmBI 1242 GAGACG CGTCTC(1/5)
BsmI 378 GAATGC GAATGC(1/-1)
Bsp1407I 2877 TGTACA T^GTACA
BspHI 3491 TCATGA T^CATGA
BspLU11I 18 ACATGT A^CATGT
BspMI 4066 GCAGGT ACCTGC(4/8)
BsrFI 2280 GCCGGC [GA]^CCGG[CT]
BsrGI 2877 TGTACA T^GTACA
BstUI 1493 CGCG CG^CG
BstXI 557 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 415 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2280 GCCGGC [GA]^CCGG[CT]
DraIII 2540 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3497 CCTTAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 346 GATATC GAT^ATC
EcoT22I 595 ATGCAT ATGCA^T
HaeII 1952 AGCGCC [GA]GCGC^[CT]
Hin1I 1196 GGCGTC G[GA]^CG[CT]C
HindIII 1443 AAGCTT A^AGCTT
MamI 1919 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1493 CGCG CG^CG
NaeI 2280 GCCGGC GCC^GGC
NdeI 3245 CATATG CA^TATG
NgoMIV 2280 GCCGGC G^CCGGC
NsiI 595 ATGCAT ATGCA^T
Psp1406I 983 AACGTT AA^CGTT
RcaI 3491 TCATGA T^CATGA
SacI 3602 GAGCTC GAGCT^C
SalI 14 GTCGAC G^TCGAC
SexAI 4234 ACCAGGT A^CC[AT]GGT
SmaI 1867 CCCGGG CCC^GGG
SspBI 2877 TGTACA T^GTACA
SspI 4082 AATATT AAT^ATT
XbaI 1200 TCTAGA T^CTAGA
XmaCI 1867 CCCGGG C^CCGGG
XmaI 1867 CCCGGG C^CCGGG
XmnI 1327 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 18 ACATGT A^C[GA][CT]GT
2775 ACGTGT
AlwNI 1339 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1951 CAGCGCCTG
AspI 2325 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3864 GACTCTGTC
AvaI 1456 CCCGAG C^[CT]CG[GA]G
1867 CCCGGG
BamHI 1858 GGATCC G^GATCC
3073 GGATCC
BcgI 3896 CGATGAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2306 GCACTCTAATCG
BsoBI 1456 CCCGAG C^[CT]CG[GA]G
1867 CCCGGG
BsrBI 1135 GAGCGG CCGCTC(-3/-3)
3959 GAGCGG
DraII 4166 GGGTCCC [GA]G^G[ACGT]CC[CT]
4199 GGGTCCT
EcoO109I 4166 GGGTCCC [GA]G^G[ACGT]CC[CT]
4199 GGGTCCT
EcoRI 3586 GAATTC G^AATTC
3845 GAATTC
HgaI 1197 GCGTC GACGC(5/10)
2203 GCGTC
HincII 14 GTCGAC GT[CT]^[GA]AC
397 GTTGAC
HindII 14 GTCGAC GT[CT]^[GA]AC
397 GTTGAC
PflFI 2325 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3864 GACTCTGTC
PpuMI 4166 GGGTCCC [GA]G^G[AT]CC[CT]
4199 GGGTCCT
SapI 1117 GAAGAGC GCTCTTC(1/4)
994 GCTCTTC
Tth111I 2325 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3864 GACTCTGTC
XcmI 44 CCATCAAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3317 CCAATCAGACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
Eco81I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII