##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AvrII BbeI
BcgI BciVI BlnI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI Eam1105I
EcoRV EcoT22I HpaI KasI KspI
MamI MfeI MluI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 329 GGTACC G^GTACC
AccI 3636 GTCGAC GT^[AC][GT]AC
AcyI 280 GACGCC G[GA]^CG[CT]C
AflII 923 CTTAAG C^TTAAG
AflIII 2692 ACATGT A^C[GA][CT]GT
Alw44I 2344 GTGCAC G^TGCAC
ApaLI 2344 GTGCAC G^TGCAC
Asp700I 2767 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 329 GGTACC G^GTACC
AviII 1746 TGCGCA TGC^GCA
BbrPI 3966 CACGTG CAC^GTG
BfrI 923 CTTAAG C^TTAAG
BsaAI 3966 CACGTG [CT]AC^GT[GA]
BsaHI 280 GACGCC G[GA]^CG[CT]C
BsaI 1473 GAGACC GGTCTC(1/5)
BspHI 750 TCATGA T^CATGA
BspLU11I 2692 ACATGT A^CATGT
BssSI 2393 CTCGTG CACGAG(-5/-1)
BstAPI 1396 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2543 TTTAAA TTT^AAA
EcoRI 554 GAATTC G^AATTC
FseI 3488 GGCCGGCC GGCCGG^CC
FspI 1746 TGCGCA TGC^GCA
Hin1I 280 GACGCC G[GA]^CG[CT]C
KpnI 329 GGTACC GGTAC^C
NheI 913 GCTAGC G^CTAGC
PmaCI 3966 CACGTG CAC^GTG
PmlI 3966 CACGTG CAC^GTG
PshAI 2494 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 750 TCATGA T^CATGA
SalI 3636 GTCGAC G^TCGAC
ScaI 489 AGTACT AGT^ACT
XcmI 811 CCACGCCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2767 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 1491 CGCG CG^CG
2488 CGCG
AccIII 507 TCCGGA T^CCGGA
2856 TCCGGA
AcsI 554 GAATTC [GA]^AATT[CT]
1196 AAATTC
Aor51HI 99 AGCGCT AGC^GCT
384 AGCGCT
ApoI 554 GAATTC [GA]^AATT[CT]
1196 AAATTC
AspI 2015 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACGTGGTC
BamHI 3115 GGATCC G^GATCC
3496 GGATCC
BbsI 2206 GAAGAC GAAGAC(2/6)
1238 GTCTTC
BbvCI 2864 GCTGAGG CCTCAGC(-5/-2)
3353 GCTGAGG
BclI 3213 TGATCA T^GATCA
3306 TGATCA
BglII 420 AGATCT A^GATCT
1590 AGATCT
BpuAI 2206 GAAGAC GAAGAC(2/6)
1238 GTCTTC
BsaWI 507 TCCGGA [AT]^CCGG[AT]
2856 TCCGGA
BseAI 507 TCCGGA T^CCGGA
2856 TCCGGA
BsiEI 182 CGGCCG CG[GA][CT]^CG
3491 CGGCCG
BsmBI 278 GAGACG CGTCTC(1/5)
476 GAGACG
BsmI 1085 GAATGC GAATGC(1/-1)
3405 GCATTC
BspEI 507 TCCGGA T^CCGGA
2856 TCCGGA
BstUI 1491 CGCG CG^CG
2488 CGCG
DrdI 200 GACCTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1718 GACCTGAGGGTC
EagI 182 CGGCCG C^GGCCG
3491 CGGCCG
EclXI 182 CGGCCG C^GGCCG
3491 CGGCCG
Eco47III 99 AGCGCT AGC^GCT
384 AGCGCT
Eco52I 182 CGGCCG C^GGCCG
3491 CGGCCG
FbaI 3213 TGATCA T^GATCA
3306 TGATCA
HindIII 2723 AAGCTT A^AGCTT
2759 AAGCTT
MroI 507 TCCGGA T^CCGGA
2856 TCCGGA
MvnI 1491 CGCG CG^CG
2488 CGCG
PflFI 2015 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACGTGGTC
PflMI 763 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1348 CCAGGCAGTGG
SacI 845 GAGCTC GAGCT^C
1213 GAGCTC
SapI 721 GAAGAGC GCTCTTC(1/4)
1540 GAAGAGC
SexAI 951 ACCTGGT A^CC[AT]GGT
2016 ACCTGGT
SmaI 2165 CCCGGG CCC^GGG
2871 CCCGGG
Tth111I 2015 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACGTGGTC
Van91I 763 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1348 CCAGGCAGTGG
XmaCI 2165 CCCGGG C^CCGGG
2871 CCCGGG
XmaI 2165 CCCGGG C^CCGGG
2871 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII