##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AvrII
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KspI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3596 GTCGAC GT^[AC][GT]AC
AccII 1709 CGCG CG^CG
AocI 525 CCTAAGG CC^T[ACGT]AGG
AspI 1476 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2871 GGCGCC GGCGC^C
BbrPI 2133 CACGTG CAC^GTG
BglII 2353 AGATCT A^GATCT
BsaWI 2089 ACCGGA [AT]^CCGG[AT]
BsiEI 3598 CGACCG CG[GA][CT]^CG
BsmI 931 GCATTC GAATGC(1/-1)
BspLU11I 89 ACATGT A^CATGT
BspMI 2009 GCAGGT ACCTGC(4/8)
BsrDI 201 CATTGC GCAATG(2/0)
BssSI 3723 CACGAG CACGAG(-5/-1)
BstUI 1709 CGCG CG^CG
Bsu36I 525 CCTAAGG CC^T[ACGT]AGG
DraI 607 TTTAAA TTT^AAA
DrdI 2475 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 525 CCTAAGG CC^T[ACGT]AGG
EcoRI 3579 GAATTC G^AATTC
HindIII 1900 AAGCTT A^AGCTT
KasI 2871 GGCGCC G^GCGCC
MslI 2006 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1709 CGCG CG^CG
NarI 2871 GGCGCC GG^CGCC
NdeI 70 CATATG CA^TATG
PaeR7I 2289 CTCGAG C^TCGAG
PflFI 1476 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2133 CACGTG CAC^GTG
PmlI 2133 CACGTG CAC^GTG
PshAI 573 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3354 CTGCAG CTGCA^G
SacI 3218 GAGCTC GAGCT^C
SalI 3596 GTCGAC G^TCGAC
SapI 962 GCTCTTC GCTCTTC(1/4)
ScaI 2221 AGTACT AGT^ACT
SfoI 2871 GGCGCC GGC^GCC
SmaI 4030 CCCGGG CCC^GGG
SpeI 106 ACTAGT A^CTAGT
SspI 4106 AATATT AAT^ATT
StuI 2956 AGGCCT AGG^CCT
Tth111I 1476 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2289 CTCGAG C^TCGAG
XmaCI 4030 CCCGGG C^CCGGG
XmaI 4030 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Acc65I 20 GGTACC G^GTACC
740 GGTACC
Asp718I 20 GGTACC G^GTACC
740 GGTACC
AspHI 2600 GAGCAC G[AT]GC[AT]^C
3218 GAGCTC
BamHI 388 GGATCC G^GATCC
881 GGATCC
BbvCI 3337 CCTCAGC CCTCAGC(-5/-2)
1769 GCTGAGG
BsiHKAI 2600 GAGCAC G[AT]GC[AT]^C
3218 GAGCTC
BsmBI 1553 CGTCTC CGTCTC(1/5)
1851 GAGACG
BsrBI 377 GAGCGG CCGCTC(-3/-3)
951 GAGCGG
BstXI 1484 CCATGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3079 CCAGCCTCCTGG
KpnI 20 GGTACC GGTAC^C
740 GGTACC
NcoI 3232 CCATGG C^CATGG
3445 CCATGG
PflMI 2714 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2954 CCAGGCCTTGG
Van91I 2714 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2954 CCAGGCCTTGG
XcmI 496 CCAGGGCTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
784 CCAGGTCTCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII