##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Aor51HI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BcgI
BfrI BlnI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoRI EcoRV FseI FspI
HpaI KpnI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmeI
PshAI PshBI PvuI RsrII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
Van91I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 3912 AACGTT AA^CGTT
AocI 3172 CCTGAGG CC^T[ACGT]AGG
ApaI 1110 GGGCCC GGGCC^C
AscI 2324 GGCGCGCC GG^CGCGCC
AspI 107 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1892 TGGCCA TGG^CCA
BbeI 3061 GGCGCC GGCGC^C
BbrPI 3089 CACGTG CAC^GTG
BglI 2346 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3743 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2095 CGGCCG CG[GA][CT]^CG
BsmI 4026 GCATTC GAATGC(1/-1)
BsrFI 1994 ACCGGC [GA]^CCGG[CT]
BstEII 1982 GGTCACC G^GT[ACGT]ACC
BstPI 1982 GGTCACC G^GT[ACGT]ACC
BstXI 2568 CCAATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3172 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1994 ACCGGC [GA]^CCGG[CT]
DraIII 3709 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2095 CGGCCG C^GGCCG
EclXI 2095 CGGCCG C^GGCCG
Eco52I 2095 CGGCCG C^GGCCG
Eco81I 3172 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1982 GGTCACC G^GT[ACGT]ACC
EcoT22I 600 ATGCAT ATGCA^T
KasI 3061 GGCGCC G^GCGCC
KspI 2164 CCGCGG CCGC^GG
MamI 3743 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1892 TGGCCA TGG^CCA
MscI 1892 TGGCCA TGG^CCA
NarI 3061 GGCGCC GG^CGCC
NcoI 1694 CCATGG C^CATGG
NheI 1241 GCTAGC G^CTAGC
NsiI 600 ATGCAT ATGCA^T
PflFI 107 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3089 CACGTG CAC^GTG
PmlI 3089 CACGTG CAC^GTG
Psp1406I 3912 AACGTT AA^CGTT
PstI 711 CTGCAG CTGCA^G
SacI 3049 GAGCTC GAGCT^C
SacII 2164 CCGCGG CCGC^GG
SalI 2076 GTCGAC G^TCGAC
SapI 2006 GCTCTTC GCTCTTC(1/4)
SfoI 3061 GGCGCC GGC^GCC
SmaI 4040 CCCGGG CCC^GGG
Tth111I 107 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1880 CCAACTCGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4040 CCCGGG C^CCGGG
XmaI 4040 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 2100 GTGCAC G^TGCAC
2298 GTGCAC
AlwNI 1818 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2708 CAGACGCTG
ApaLI 2100 GTGCAC G^TGCAC
2298 GTGCAC
BbsI 267 GAAGAC GAAGAC(2/6)
1307 GTCTTC
BclI 229 TGATCA T^GATCA
2976 TGATCA
BglII 2198 AGATCT A^GATCT
3742 AGATCT
BlpI 2334 GCTGAGC GC^T[ACGT]AGC
3051 GCTCAGC
Bpu1102I 2334 GCTGAGC GC^T[ACGT]AGC
3051 GCTCAGC
BpuAI 267 GAAGAC GAAGAC(2/6)
1307 GTCTTC
BspHI 673 TCATGA T^CATGA
1247 TCATGA
BsrDI 211 CATTGC GCAATG(2/0)
367 GCAATG
BssHII 2325 GCGCGC G^CGCGC
2917 GCGCGC
BssSI 1965 CACGAG CACGAG(-5/-1)
4031 CTCGTG
CelII 2334 GCTGAGC GC^T[ACGT]AGC
3051 GCTCAGC
FbaI 229 TGATCA T^GATCA
2976 TGATCA
HindIII 250 AAGCTT A^AGCTT
471 AAGCTT
NdeI 2405 CATATG CA^TATG
3643 CATATG
NspI 602 GCATGT [GA]CATG^[CT]
3768 ACATGC
PaeR7I 1165 CTCGAG C^TCGAG
1977 CTCGAG
PvuII 639 CAGCTG CAG^CTG
2190 CAGCTG
RcaI 673 TCATGA T^CATGA
1247 TCATGA
SfaNI 599 GATGC GCATC(5/9)
2825 GCATC
SspI 2962 AATATT AAT^ATT
3666 AATATT
XbaI 1457 TCTAGA T^CTAGA
3745 TCTAGA
XhoI 1165 CTCGAG C^TCGAG
1977 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StuI StyI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI