##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AclI AflII AscI
AspI AviII BamHI BbvCI BcgI
BfrI BseAI BsiWI BspDI BspEI
BspHI BssHII BssSI BstBI ClaI
CpoI DraIII EcoRI EcoT22I FseI
FspI HpaI MfeI MroI MunI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PmeI Psp1406I
RcaI RsrII SacI SalI SfiI
SfuI SgrAI SpeI SphI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 973 GGTACC G^GTACC
AccI 201 GTATAC GT^[AC][GT]AC
AgeI 901 ACCGGT A^CCGGT
AhdI 1597 GACGTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 164 GTGCAC G^TGCAC
Aor51HI 1400 AGCGCT AGC^GCT
ApaLI 164 GTGCAC G^TGCAC
Asp700I 3002 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 973 GGTACC G^GTACC
AspEI 1597 GACGTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 411 CACGTG CAC^GTG
BciVI 1462 GTATCC GTATCC(6/5)
BclI 2431 TGATCA T^GATCA
BlpI 6 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
BsaBI 1783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1533 GCATTC GAATGC(1/-1)
BspMI 3039 GCAGGT ACCTGC(4/8)
BsrBI 302 GAGCGG CCGCTC(-3/-3)
Bst1107I 201 GTATAC GTA^TAC
BstEII 1309 GGTTACC G^GT[ACGT]ACC
BstPI 1309 GGTTACC G^GT[ACGT]ACC
BstZ17I 201 GTATAC GTA^TAC
CelII 6 GCTGAGC GC^T[ACGT]AGC
EagI 157 CGGCCG C^GGCCG
Eam1105I 1597 GACGTTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 157 CGGCCG C^GGCCG
Eco47III 1400 AGCGCT AGC^GCT
Eco52I 157 CGGCCG C^GGCCG
EcoO65I 1309 GGTTACC G^GT[ACGT]ACC
EcoRV 2541 GATATC GAT^ATC
FbaI 2431 TGATCA T^GATCA
HindIII 3799 AAGCTT A^AGCTT
KpnI 973 GGTACC GGTAC^C
KspI 814 CCGCGG CCGC^GG
MamI 1783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3296 ACGCGT A^CGCGT
NheI 1503 GCTAGC G^CTAGC
PaeR7I 1561 CTCGAG C^TCGAG
PflMI 2136 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 901 ACCGGT A^CCGGT
PmaCI 411 CACGTG CAC^GTG
PmlI 411 CACGTG CAC^GTG
PshAI 3146 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 381 CTGCAG CTGCA^G
PvuI 214 CGATCG CGAT^CG
PvuII 1672 CAGCTG CAG^CTG
SacII 814 CCGCGG CCGC^GG
SapI 748 GCTCTTC GCTCTTC(1/4)
SbfI 380 CCTGCAGG CCTGCA^GG
SexAI 196 ACCTGGT A^CC[AT]GGT
SmaI 1041 CCCGGG CCC^GGG
SnaBI 1769 TACGTA TAC^GTA
Sse8387I 380 CCTGCAGG CCTGCA^GG
StuI 2563 AGGCCT AGG^CCT
SwaI 2334 ATTTAAAT ATTT^AAAT
Van91I 2136 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1561 CTCGAG C^TCGAG
XmaCI 1041 CCCGGG C^CCGGG
XmaI 1041 CCCGGG C^CCGGG
XmnI 3002 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvrII 2876 CCTAGG C^CTAGG
3139 CCTAGG
BbsI 3893 GAAGAC GAAGAC(2/6)
4289 GAAGAC
BlnI 2876 CCTAGG C^CTAGG
3139 CCTAGG
BpuAI 3893 GAAGAC GAAGAC(2/6)
4289 GAAGAC
BsaI 2703 GAGACC GGTCTC(1/5)
1604 GGTCTC
BsaWI 190 ACCGGA [AT]^CCGG[AT]
901 ACCGGT
BsgI 186 CTGCAC GTGCAG(16/14)
321 CTGCAC
BsmBI 2899 CGTCTC CGTCTC(1/5)
3144 GAGACG
Bsp1407I 3631 TGTACA T^GTACA
4281 TGTACA
BspLU11I 1302 ACATGT A^CATGT
1875 ACATGT
BsrDI 1835 CATTGC GCAATG(2/0)
2863 CATTGC
BsrFI 730 ACCGGC [GA]^CCGG[CT]
901 ACCGGT
BsrGI 3631 TGTACA T^GTACA
4281 TGTACA
BstXI 25 CCATGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2653 CCAGCACTTTGG
Cfr10I 730 ACCGGC [GA]^CCGG[CT]
901 ACCGGT
DrdI 1145 GACAGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1567 GACACCACAGTC
EarI 749 CTCTTC CTCTTC(1/4)
1706 CTCTTC
HincII 1590 GTCAAC GT[CT]^[GA]AC
1644 GTCAAC
HindII 1590 GTCAAC GT[CT]^[GA]AC
1644 GTCAAC
Ksp632I 749 CTCTTC CTCTTC(1/4)
1706 CTCTTC
NdeI 3782 CATATG CA^TATG
4196 CATATG
PpuMI 491 GGGACCC [GA]G^G[AT]CC[CT]
2176 AGGACCT
SmlI 1561 CTCGAG C^T[CT][GA]AG
2297 CTTGAG
SspBI 3631 TGTACA T^GTACA
4281 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII