##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AgeI AseI AsnI
Asp718I AviII BbeI BbrPI BbvCI
BcgI BsaAI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EcoNI EcoO65I
FseI FspI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2353 GACGTC GACGT^C
AccI 2025 GTCTAC GT^[AC][GT]AC
AcyI 2353 GACGTC G[GA]^CG[CT]C
AflIII 2138 ACATGT A^C[GA][CT]GT
AhdI 2241 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 862 AGCGCT AGC^GCT
AscI 200 GGCGCGCC GG^CGCGCC
Asp700I 28 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2241 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3997 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 372 CCTAGG C^CTAGG
BanI 1803 GGTGCC G^G[CT][GA]CC
BciVI 2895 GTATCC GTATCC(6/5)
BglI 327 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 372 CCTAGG C^CTAGG
BlpI 3683 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3683 GCTGAGC GC^T[ACGT]AGC
BsaHI 2353 GACGTC G[GA]^CG[CT]C
BsaWI 430 ACCGGA [AT]^CCGG[AT]
BsiEI 295 CGGCCG CG[GA][CT]^CG
BspLU11I 2138 ACATGT A^CATGT
BspMI 259 GCAGGT ACCTGC(4/8)
CelII 3683 GCTGAGC GC^T[ACGT]AGC
EagI 295 CGGCCG C^GGCCG
Eam1105I 2241 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 295 CGGCCG C^GGCCG
Eco47III 862 AGCGCT AGC^GCT
Eco52I 295 CGGCCG C^GGCCG
Hin1I 2353 GACGTC G[GA]^CG[CT]C
HpaI 3473 GTTAAC GTT^AAC
NaeI 346 GCCGGC GCC^GGC
NgoMIV 346 GCCGGC G^CCGGC
PaeR7I 381 CTCGAG C^TCGAG
PflFI 3997 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3931 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3235 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1877 CTGCAG CTGCA^G
SapI 2614 GAAGAGC GCTCTTC(1/4)
ScaI 3333 AGTACT AGT^ACT
StuI 3156 AGGCCT AGG^CCT
Tth111I 3997 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3931 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2159 CCAGATATGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 381 CTCGAG C^TCGAG
XmnI 28 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AclI 1993 AACGTT AA^CGTT
3174 AACGTT
AflII 2604 CTTAAG C^TTAAG
3775 CTTAAG
Alw44I 3298 GTGCAC G^TGCAC
3608 GTGCAC
AlwNI 523 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
3932 CAGAAGCTG
AocI 1116 CCTGAGG CC^T[ACGT]AGG
3121 CCTAAGG
ApaI 210 GGGCCC GGGCC^C
1122 GGGCCC
ApaLI 3298 GTGCAC G^TGCAC
3608 GTGCAC
AvaII 253 GGACC G^G[AT]CC
3236 GGTCC
BclI 1384 TGATCA T^GATCA
1423 TGATCA
BfrI 2604 CTTAAG C^TTAAG
3775 CTTAAG
BglII 449 AGATCT A^GATCT
1361 AGATCT
BsgI 3969 CTGCAC GTGCAG(16/14)
981 GTGCAG
BsmFI 1682 GGGAC GGGAC(10/14)
3237 GTCCC
Bsp1407I 969 TGTACA T^GTACA
2655 TGTACA
BsrBI 249 GAGCGG CCGCTC(-3/-3)
766 GAGCGG
BsrGI 969 TGTACA T^GTACA
2655 TGTACA
BssHII 2 GCGCGC G^CGCGC
201 GCGCGC
Bsu36I 1116 CCTGAGG CC^T[ACGT]AGG
3121 CCTAAGG
DsaI 214 CCGCGG C^C[GA][CT]GG
328 CCGCGG
Eco81I 1116 CCTGAGG CC^T[ACGT]AGG
3121 CCTAAGG
EcoRI 3109 GAATTC G^AATTC
3786 GAATTC
EcoRV 501 GATATC GAT^ATC
2184 GATATC
FbaI 1384 TGATCA T^GATCA
1423 TGATCA
HaeII 312 AGCGCC [GA]GCGC^[CT]
862 AGCGCT
HgaI 318 GCGTC GACGC(5/10)
3130 GCGTC
HincII 1929 GTTGAC GT[CT]^[GA]AC
3473 GTTAAC
HindII 1929 GTTGAC GT[CT]^[GA]AC
3473 GTTAAC
KspI 214 CCGCGG CCGC^GG
328 CCGCGG
MslI 705 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2490 CACTTGGATG
Psp1406I 1993 AACGTT AA^CGTT
3174 AACGTT
SacII 214 CCGCGG CCGC^GG
328 CCGCGG
SmaI 7 CCCGGG CCC^GGG
207 CCCGGG
SphI 2149 GCATGC GCATG^C
3724 GCATGC
SspBI 969 TGTACA T^GTACA
2655 TGTACA
XbaI 1322 TCTAGA T^CTAGA
3790 TCTAGA
XmaCI 7 CCCGGG C^CCGGG
207 CCCGGG
XmaI 7 CCCGGG C^CCGGG
207 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI BalI
BamHI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I BspHI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII