##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Aor51HI AscI AspI
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiWI BspDI BspEI BsrFI BssHII
Bst1107I BstBI BstUI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRV
FbaI FseI HaeII Hin1I KasI
KspI MamI MflI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SfoI SfuI SgrAI SmaI
SnaBI SpeI StuI SwaI Tth111I
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2730 GGTACC G^GTACC
Alw44I 4149 GTGCAC G^TGCAC
AocI 1722 CCTAAGG CC^T[ACGT]AGG
ApaI 2469 GGGCCC GGGCC^C
ApaLI 4149 GTGCAC G^TGCAC
Asp718I 2730 GGTACC G^GTACC
AvaI 1452 CTCGAG C^[CT]CG[GA]G
BalI 1105 TGGCCA TGG^CCA
BbsI 1994 GAAGAC GAAGAC(2/6)
BbvCI 2995 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1994 GAAGAC GAAGAC(2/6)
BsaWI 1371 TCCGGT [AT]^CCGG[AT]
BseRI 3662 GAGGAG GAGGAG(10/8)
BsmBI 199 CGTCTC CGTCTC(1/5)
BsoBI 1452 CTCGAG C^[CT]CG[GA]G
BspHI 4040 TCATGA T^CATGA
BspLU11I 2179 ACATGT A^CATGT
BssSI 1192 CACGAG CACGAG(-5/-1)
BstXI 2925 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1722 CCTAAGG CC^T[ACGT]AGG
DraIII 1468 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1105 TGGCCA [CT]^GGCC[GA]
Eco81I 1722 CCTAAGG CC^T[ACGT]AGG
EcoNI 2920 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1771 GACGC GACGC(5/10)
HindIII 3556 AAGCTT A^AGCTT
HpaI 850 GTTAAC GTT^AAC
KpnI 2730 GGTACC GGTAC^C
MluNI 1105 TGGCCA TGG^CCA
MscI 1105 TGGCCA TGG^CCA
PaeR7I 1452 CTCGAG C^TCGAG
PshAI 4091 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4040 TCATGA T^CATGA
SacI 62 GAGCTC GAGCT^C
SapI 912 GCTCTTC GCTCTTC(1/4)
SbfI 2960 CCTGCAGG CCTGCA^GG
ScaI 2770 AGTACT AGT^ACT
SexAI 108 ACCTGGT A^CC[AT]GGT
SfiI 10 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 147 GCATGC GCATG^C
Sse8387I 2960 CCTGCAGG CCTGCA^GG
XhoI 1452 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 752 GTCTAC GT^[AC][GT]AC
1130 GTAGAC
AhdI 266 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2883 GACGGGCTGTC
AspEI 266 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2883 GACGGGCTGTC
AviII 125 TGCGCA TGC^GCA
1479 TGCGCA
BglI 11 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2959 GCCTGCAGGGC
BsaAI 1705 TACGTG [CT]AC^GT[GA]
2381 TACGTG
BsiEI 1074 CGACCG CG[GA][CT]^CG
1982 CGACCG
Bsp1407I 2432 TGTACA T^GTACA
4026 TGTACA
BspMI 2968 GCAGGT ACCTGC(4/8)
3087 GCAGGT
BsrBI 730 CCGCTC CCGCTC(-3/-3)
1042 CCGCTC
BsrGI 2432 TGTACA T^GTACA
4026 TGTACA
BstAPI 142 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2936 GCAGGGCCTGC
BstEII 1024 GGTGACC G^GT[ACGT]ACC
2789 GGTCACC
BstPI 1024 GGTGACC G^GT[ACGT]ACC
2789 GGTCACC
Eam1105I 266 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2883 GACGGGCTGTC
EcoO65I 1024 GGTGACC G^GT[ACGT]ACC
2789 GGTCACC
EcoRI 2155 GAATTC G^AATTC
2903 GAATTC
EcoT22I 1366 ATGCAT ATGCA^T
2909 ATGCAT
FspI 125 TGCGCA TGC^GCA
1479 TGCGCA
HincII 850 GTTAAC GT[CT]^[GA]AC
2880 GTTGAC
HindII 850 GTTAAC GT[CT]^[GA]AC
2880 GTTGAC
MfeI 3183 CAATTG C^AATTG
4171 CAATTG
MunI 3183 CAATTG C^AATTG
4171 CAATTG
NcoI 24 CCATGG C^CATGG
2516 CCATGG
NsiI 1366 ATGCAT ATGCA^T
2909 ATGCAT
PflMI 2516 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3857 CCATACTCTGG
PvuII 1740 CAGCTG CAG^CTG
3067 CAGCTG
SspBI 2432 TGTACA T^GTACA
4026 TGTACA
Van91I 2516 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3857 CCATACTCTGG
XbaI 1646 TCTAGA T^CTAGA
1767 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmnI