##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI AscI AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BglII BlnI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfoI SfuI
SgrAI SnaBI Sse8387I SspI StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 701 GTAGAC GT^[AC][GT]AC
AflII 3241 CTTAAG C^TTAAG
AspI 3185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 505 CCCGGG C^[CT]CG[GA]G
BciVI 3827 GTATCC GTATCC(6/5)
BfrI 3241 CTTAAG C^TTAAG
BlpI 2591 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2591 GCTGAGC GC^T[ACGT]AGC
BsaAI 3717 TACGTG [CT]AC^GT[GA]
BsoBI 505 CCCGGG C^[CT]CG[GA]G
BsrFI 1610 GCCGGT [GA]^CCGG[CT]
BssSI 612 CACGAG CACGAG(-5/-1)
CelII 2591 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1610 GCCGGT [GA]^CCGG[CT]
EcoRI 1766 GAATTC G^AATTC
FauI 1702 CCCGC CCCGC(4/6)
HgaI 3456 GACGC GACGC(5/10)
PflFI 3185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2867 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1294 GAGCTC GAGCT^C
SapI 1291 GAAGAGC GCTCTTC(1/4)
SfiI 1691 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 505 CCCGGG CCC^GGG
SmlI 3241 CTTAAG C^T[CT][GA]AG
SpeI 3577 ACTAGT A^CTAGT
Tth111I 3185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3818 TCTAGA T^CTAGA
XcmI 3187 CCATGTCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 505 CCCGGG C^CCGGG
XmaI 505 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1058 GGTACC G^GTACC
3429 GGTACC
Alw44I 891 GTGCAC G^TGCAC
3555 GTGCAC
ApaLI 891 GTGCAC G^TGCAC
3555 GTGCAC
AseI 158 ATTAAT AT^TAAT
1904 ATTAAT
AsnI 158 ATTAAT AT^TAAT
1904 ATTAAT
Asp700I 1337 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1766 GAATTCTTTC
Asp718I 1058 GGTACC G^GTACC
3429 GGTACC
BbvCI 745 CCTCAGC CCTCAGC(-5/-2)
1421 GCTGAGG
BsaBI 1385 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3023 GATGTGGATC
BsaI 3206 GAGACC GGTCTC(1/5)
3846 GAGACC
BsaWI 237 ACCGGA [AT]^CCGG[AT]
2283 ACCGGA
Bsp1407I 1527 TGTACA T^GTACA
4088 TGTACA
BsrGI 1527 TGTACA T^GTACA
4088 TGTACA
EcoRV 2495 GATATC GAT^ATC
3530 GATATC
HindIII 3491 AAGCTT A^AGCTT
3637 AAGCTT
KpnI 1058 GGTACC GGTAC^C
3429 GGTACC
MamI 1385 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3023 GATGTGGATC
PflMI 2432 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3019 CCAAGATGTGG
PshBI 158 ATTAAT AT^TAAT
1904 ATTAAT
PvuII 748 CAGCTG CAG^CTG
1830 CAGCTG
SexAI 1054 ACCTGGT A^CC[AT]GGT
1061 ACCTGGT
SspBI 1527 TGTACA T^GTACA
4088 TGTACA
Van91I 2432 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3019 CCAAGATGTGG
XmnI 1337 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1766 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII