##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AviII
BciVI BfrI BsaBI BsaWI BseAI
BsmI BspEI BssHII BstBI BstEII
BstPI DraI Eam1105I EcoO65I EcoRV
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI SalI SbfI SfuI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2635 GACGTC GACGT^C
AocI 3789 CCTGAGG CC^T[ACGT]AGG
AvrII 4202 CCTAGG C^CTAGG
BbrPI 3349 CACGTG CAC^GTG
BbvCI 4428 GCTGAGG CCTCAGC(-5/-2)
BclI 2816 TGATCA T^GATCA
BlnI 4202 CCTAGG C^CTAGG
BsiEI 4819 CGGCCG CG[GA][CT]^CG
BsiWI 1361 CGTACG C^GTACG
Bsp1407I 4759 TGTACA T^GTACA
BspDI 250 ATCGAT AT^CGAT
BsrDI 2601 CATTGC GCAATG(2/0)
BsrGI 4759 TGTACA T^GTACA
Bst1107I 4215 GTATAC GTA^TAC
BstZ17I 4215 GTATAC GTA^TAC
Bsu36I 3789 CCTGAGG CC^T[ACGT]AGG
ClaI 250 ATCGAT AT^CGAT
CpoI 2353 CGGACCG CG^G[AT]CCG
DrdI 1297 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4819 CGGCCG C^GGCCG
EclXI 4819 CGGCCG C^GGCCG
Eco52I 4819 CGGCCG C^GGCCG
Eco81I 3789 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1193 ATGCAT ATGCA^T
FbaI 2816 TGATCA T^GATCA
FseI 625 GGCCGGCC GGCCGG^CC
KspI 68 CCGCGG CCGC^GG
NheI 3271 GCTAGC G^CTAGC
NsiI 1193 ATGCAT ATGCA^T
PmaCI 3349 CACGTG CAC^GTG
PmlI 3349 CACGTG CAC^GTG
RsrII 2353 CGGACCG CG^G[AT]CCG
SacII 68 CCGCGG CCGC^GG
ScaI 2462 AGTACT AGT^ACT
SexAI 167 ACCAGGT A^CC[AT]GGT
SfiI 64 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2995 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 4847 CCCGGG CCC^GGG
SphI 4901 GCATGC GCATG^C
SspBI 4759 TGTACA T^GTACA
StuI 4510 AGGCCT AGG^CCT
XbaI 4636 TCTAGA T^CTAGA
XmaCI 4847 CCCGGG C^CCGGG
XmaI 4847 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 889 GGTACC G^GTACC
3318 GGTACC
Asp700I 228 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2775 GAACTGCTTC
Asp718I 889 GGTACC G^GTACC
3318 GGTACC
BalI 671 TGGCCA TGG^CCA
1430 TGGCCA
BamHI 1213 GGATCC G^GATCC
4005 GGATCC
BcgI 4692 CGATTCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2880 GCACCTGGCTCG
BsmBI 4823 CGTCTC CGTCTC(1/5)
1420 GAGACG
BspHI 224 TCATGA T^CATGA
2819 TCATGA
BspLU11I 3146 ACATGT A^CATGT
3707 ACATGT
DraIII 775 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2620 CACCTCGTG
EcoRI 405 GAATTC G^AATTC
502 GAATTC
KpnI 889 GGTACC GGTAC^C
3318 GGTACC
MluNI 671 TGGCCA TGG^CCA
1430 TGGCCA
MscI 671 TGGCCA TGG^CCA
1430 TGGCCA
RcaI 224 TCATGA T^CATGA
2819 TCATGA
SapI 975 GAAGAGC GCTCTTC(1/4)
285 GCTCTTC
SspI 3888 AATATT AAT^ATT
5029 AATATT
XmnI 228 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2775 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII