##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AspI AvaI AvaII AviII AvrII
BalI BbeI BbvCI BcgI BglI
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I CfoI Cfr10I ClaI CpoI
DraII EagI EclXI Eco47III Eco52I
EcoO109I EcoO65I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PvuI RsrII SacII
SbfI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2349 GGTACC G^GTACC
AflIII 2109 ACATGT A^C[GA][CT]GT
AhdI 3232 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2926 CCTAAGG CC^T[ACGT]AGG
Asp718I 2349 GGTACC G^GTACC
AspEI 3232 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2349 GGTACC G^G[CT][GA]CC
BbrPI 1460 CACGTG CAC^GTG
BclI 3702 TGATCA T^GATCA
BcnI 3653 CCCGG CC^[GC]GG
BlpI 1021 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1021 GCTAAGC GC^T[ACGT]AGC
BsaAI 1460 CACGTG [CT]AC^GT[GA]
BsgI 2267 GTGCAG GTGCAG(16/14)
BspLU11I 2109 ACATGT A^CATGT
BstAPI 1115 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2718 TTCGAA TT^CGAA
Bsu36I 2926 CCTAAGG CC^T[ACGT]AGG
CelII 1021 GCTAAGC GC^T[ACGT]AGC
DrdI 346 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1739 CCACGG C^C[GA][CT]GG
Eam1105I 3232 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2926 CCTAAGG CC^T[ACGT]AGG
EcoNI 970 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1177 GAATTC G^AATTC
EcoT14I 3886 CCTTGG C^C[AT][AT]GG
FbaI 3702 TGATCA T^GATCA
HapII 3654 CCGG C^CGG
HindIII 1541 AAGCTT A^AGCTT
HpaII 3654 CCGG C^CGG
KpnI 2349 GGTACC GGTAC^C
MspI 3654 CCGG C^CGG
NciI 3653 CCCGG CC^[GC]GG
NspV 2718 TTCGAA TT^CGAA
PflMI 2289 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1460 CACGTG CAC^GTG
PmlI 1460 CACGTG CAC^GTG
PstI 3502 CTGCAG CTGCA^G
SacI 510 GAGCTC GAGCT^C
SalI 1523 GTCGAC G^TCGAC
ScaI 2767 AGTACT AGT^ACT
SfuI 2718 TTCGAA TT^CGAA
SpeI 2776 ACTAGT A^CTAGT
StyI 3886 CCTTGG C^C[AT][AT]GG
Van91I 2289 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 648 TCTAGA T^CTAGA
XcmI 3877 CCAGGGGTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1523 GTCGAC GT^[AC][GT]AC
1825 GTAGAC
AclI 865 AACGTT AA^CGTT
2933 AACGTT
AflII 2190 CTTAAG C^TTAAG
2314 CTTAAG
BamHI 180 GGATCC G^GATCC
2502 GGATCC
BanII 510 GAGCTC G[GA]GC[CT]^C
2340 GAGCCC
BciVI 3009 GGATAC GTATCC(6/5)
3667 GTATCC
BfrI 2190 CTTAAG C^TTAAG
2314 CTTAAG
BglII 651 AGATCT A^GATCT
2464 AGATCT
BsaI 2875 GAGACC GGTCTC(1/5)
1077 GGTCTC
BseRI 2954 CTCCTC GAGGAG(10/8)
191 GAGGAG
BsmFI 979 GGGAC GGGAC(10/14)
3650 GTCCC
Bsp1407I 1343 TGTACA T^GTACA
3693 TGTACA
BsrDI 350 GCAATG GCAATG(2/0)
718 GCAATG
BsrGI 1343 TGTACA T^GTACA
3693 TGTACA
Cfr13I 833 GGGCC G^G[ACGT]CC
3454 GGGCC
DraIII 1925 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4314 CACATAGTG
EaeI 1810 CGGCCA [CT]^GGCC[GA]
1950 TGGCCG
EcoRV 2860 GATATC GAT^ATC
4371 GATATC
EcoT22I 95 ATGCAT ATGCA^T
1672 ATGCAT
MfeI 584 CAATTG C^AATTG
1705 CAATTG
MunI 584 CAATTG C^AATTG
1705 CAATTG
NsiI 95 ATGCAT ATGCA^T
1672 ATGCAT
Psp1406I 865 AACGTT AA^CGTT
2933 AACGTT
PvuII 1163 CAGCTG CAG^CTG
1243 CAGCTG
SapI 2990 GCTCTTC GCTCTTC(1/4)
4027 GCTCTTC
Sau96I 833 GGGCC G^G[ACGT]CC
3454 GGGCC
SexAI 1073 ACCAGGT A^CC[AT]GGT
3323 ACCAGGT
SmlI 2190 CTTAAG C^T[CT][GA]AG
2314 CTTAAG
SspBI 1343 TGTACA T^GTACA
3693 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI RcaI RsaI Sau3AI ScrFI
SfaNI SfcI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI