##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI Alw44I ApaLI
AscI Asp700I AspI AviII BsiWI
BspHI Bst1107I BstBI BstEII BstPI
BstZ17I CpoI EcoO65I EcoRI FseI
FspI HpaI KspI MfeI MluI
MunI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfuI SnaBI SpeI
SphI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 785 GGTACC G^GTACC
AccI 1187 GTCTAC GT^[AC][GT]AC
AccIII 1603 TCCGGA T^CCGGA
AflII 2231 CTTAAG C^TTAAG
Aor51HI 4137 AGCGCT AGC^GCT
Asp718I 785 GGTACC G^GTACC
AvrII 5301 CCTAGG C^CTAGG
BalI 3130 TGGCCA TGG^CCA
BbrPI 4703 CACGTG CAC^GTG
BbvCI 1507 CCTCAGC CCTCAGC(-5/-2)
BcgI 613 GCAAATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2231 CTTAAG C^TTAAG
BglII 5348 AGATCT A^GATCT
BlnI 5301 CCTAGG C^CTAGG
BlpI 2625 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2625 GCTCAGC GC^T[ACGT]AGC
BseAI 1603 TCCGGA T^CCGGA
BspDI 1304 ATCGAT AT^CGAT
BspEI 1603 TCCGGA T^CCGGA
BspLU11I 5113 ACATGT A^CATGT
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BssSI 4664 CACGAG CACGAG(-5/-1)
CelII 2625 GCTCAGC GC^T[ACGT]AGC
ClaI 1304 ATCGAT AT^CGAT
DraIII 2850 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4137 AGCGCT AGC^GCT
EcoRV 4052 GATATC GAT^ATC
EcoT22I 1482 ATGCAT ATGCA^T
HindIII 244 AAGCTT A^AGCTT
KpnI 785 GGTACC GGTAC^C
MluNI 3130 TGGCCA TGG^CCA
MroI 1603 TCCGGA T^CCGGA
MscI 3130 TGGCCA TGG^CCA
NheI 5465 GCTAGC G^CTAGC
NsiI 1482 ATGCAT ATGCA^T
PaeR7I 4175 CTCGAG C^TCGAG
PmaCI 4703 CACGTG CAC^GTG
PmlI 4703 CACGTG CAC^GTG
PshAI 554 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1743 ACCTGGT A^CC[AT]GGT
SgrAI 4350 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 4175 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 2000 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4691 GACGTGAAGTC
AspEI 2000 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4691 GACGTGAAGTC
BsaBI 2525 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5765 GATGTTTATC
BsaI 446 GAGACC GGTCTC(1/5)
3108 GAGACC
BsmI 3672 GCATTC GAATGC(1/-1)
5659 GCATTC
BstXI 1286 CCATATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2946 CCAGTGATGTGG
DraI 5287 TTTAAA TTT^AAA
5526 TTTAAA
DrdI 1894 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4892 GACTTGGATGTC
Eam1105I 2000 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4691 GACGTGAAGTC
EcoNI 364 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5172 CCTCCTGCAGG
MamI 2525 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5765 GATGTTTATC
NdeI 1287 CATATG CA^TATG
2460 CATATG
PflMI 1952 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4673 CCAAGGCCTGG
SbfI 1438 CCTGCAGG CCTGCA^GG
5175 CCTGCAGG
Sse8387I 1438 CCTGCAGG CCTGCA^GG
5175 CCTGCAGG
Van91I 1952 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4673 CCAAGGCCTGG
XbaI 5060 TCTAGA T^CTAGA
5761 TCTAGA
XcmI 1414 CCACACAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4037 CCAGGCCCAACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AseI AsnI AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbsI BbvI BciVI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrFI
BsrGI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PshBI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI