##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BciVI BclI
BfrI BlnI BsaBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2217 GTCGAC GT^[AC][GT]AC
AccIII 1990 TCCGGA T^CCGGA
AcsI 1286 AAATTT [GA]^AATT[CT]
AflIII 622 ACGTGT A^C[GA][CT]GT
ApoI 1286 AAATTT [GA]^AATT[CT]
AviII 3238 TGCGCA TGC^GCA
BcgI 566 GCAAGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 882 TACGTG [CT]AC^GT[GA]
BseAI 1990 TCCGGA T^CCGGA
BsiEI 23 CGGCCG CG[GA][CT]^CG
BsiWI 880 CGTACG C^GTACG
BspEI 1990 TCCGGA T^CCGGA
BspHI 2984 TCATGA T^CATGA
DraIII 1851 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 23 CGGCCG C^GGCCG
EclXI 23 CGGCCG C^GGCCG
Eco52I 23 CGGCCG C^GGCCG
FspI 3238 TGCGCA TGC^GCA
HincII 2217 GTCGAC GT[CT]^[GA]AC
HindII 2217 GTCGAC GT[CT]^[GA]AC
MroI 1990 TCCGGA T^CCGGA
NdeI 2056 CATATG CA^TATG
PflMI 3669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2468 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2984 TCATGA T^CATGA
SalI 2217 GTCGAC G^TCGAC
SexAI 1852 ACCTGGT A^CC[AT]GGT
SfiI 3568 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I 3669 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 1033 AGCGCT AGC^GCT
2398 AGCGCT
BalI 232 TGGCCA TGG^CCA
3324 TGGCCA
BbvCI 326 GCTGAGG CCTCAGC(-5/-2)
3826 GCTGAGG
BfaI 1185 CTAG C^TAG
3705 CTAG
BglII 796 AGATCT A^GATCT
2614 AGATCT
BlpI 759 GCTCAGC GC^T[ACGT]AGC
1707 GCTGAGC
Bpu1102I 759 GCTCAGC GC^T[ACGT]AGC
1707 GCTGAGC
BsgI 3487 CTGCAC GTGCAG(16/14)
885 GTGCAG
CelII 759 GCTCAGC GC^T[ACGT]AGC
1707 GCTGAGC
Eco47III 1033 AGCGCT AGC^GCT
2398 AGCGCT
EcoT14I 1672 CCATGG C^C[AT][AT]GG
2581 CCATGG
MaeI 1185 CTAG C^TAG
3705 CTAG
MluNI 232 TGGCCA TGG^CCA
3324 TGGCCA
MscI 232 TGGCCA TGG^CCA
3324 TGGCCA
MseI 397 TTAA T^TAA
4196 TTAA
NaeI 21 GCCGGC GCC^GGC
3406 GCCGGC
NcoI 1672 CCATGG C^CATGG
2581 CCATGG
NgoMIV 21 GCCGGC G^CCGGC
3406 GCCGGC
StuI 1517 AGGCCT AGG^CCT
4131 AGGCCT
StyI 1672 CCATGG C^C[AT][AT]GG
2581 CCATGG
TfiI 2306 GAATC G^A[AT]TC
2879 GATTC
Tru9I 397 TTAA T^TAA
4196 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI