##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AscI AseI AsnI Asp700I
BciVI BclI BfrI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DrdI
EcoRV EcoT22I FbaI HincII HindII
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RcaI RsrII SacII SalI SfuI
SgrAI SpeI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 667 GGTACC G^GTACC
AccI 925 GTCTAC GT^[AC][GT]AC
AhdI 784 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5073 CCTGAGG CC^T[ACGT]AGG
Asp718I 667 GGTACC G^GTACC
AspEI 784 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3938 TGCGCA TGC^GCA
AvrII 5449 CCTAGG C^CTAGG
BlnI 5449 CCTAGG C^CTAGG
BsmI 3463 GCATTC GAATGC(1/-1)
Bsu36I 5073 CCTGAGG CC^T[ACGT]AGG
DraI 5904 TTTAAA TTT^AAA
Eam1105I 784 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5073 CCTGAGG CC^T[ACGT]AGG
EcoRI 1681 GAATTC G^AATTC
FspI 3938 TGCGCA TGC^GCA
HindIII 4582 AAGCTT A^AGCTT
KpnI 667 GGTACC GGTAC^C
PvuI 3684 CGATCG CGAT^CG
SacI 3787 GAGCTC GAGCT^C
SapI 2484 GAAGAGC GCTCTTC(1/4)
ScaI 2135 AGTACT AGT^ACT
SnaBI 4066 TACGTA TAC^GTA
SphI 4487 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1681 GAATTC [GA]^AATT[CT]
1755 GAATTT
ApoI 1681 GAATTC [GA]^AATT[CT]
1755 GAATTT
AspI 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACTGTGTC
BbrPI 2934 CACGTG CAC^GTG
2961 CACGTG
BcgI 299 CGAGCCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4276 CGACAGCGCTGC
BglII 2588 AGATCT A^GATCT
5240 AGATCT
Bsp1407I 2207 TGTACA T^GTACA
3185 TGTACA
BsrGI 2207 TGTACA T^GTACA
3185 TGTACA
FseI 412 GGCCGGCC GGCCGG^CC
416 GGCCGGCC
MseI 1154 TTAA T^TAA
5905 TTAA
PflFI 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACTGTGTC
PmaCI 2934 CACGTG CAC^GTG
2961 CACGTG
PmlI 2934 CACGTG CAC^GTG
2961 CACGTG
SbfI 1587 CCTGCAGG CCTGCA^GG
2151 CCTGCAGG
SexAI 1649 ACCTGGT A^CC[AT]GGT
5087 ACCTGGT
Sse8387I 1587 CCTGCAGG CCTGCA^GG
2151 CCTGCAGG
SspBI 2207 TGTACA T^GTACA
3185 TGTACA
Tru9I 1154 TTAA T^TAA
5905 TTAA
Tth111I 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI