##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AccIII AflII AgeI Alw44I
ApaLI Asp718I AvrII BbrPI BciVI
BfrI BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
ClaI CpoI EcoRI FseI HindIII
KpnI MamI MfeI MluI MroI
MunI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SalI ScaI SnaBI
SphI SspBI StuI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2758 GACGTC GACGT^C
AclI 6050 AACGTT AA^CGTT
AscI 1999 GGCGCGCC GG^CGCGCC
AviII 3196 TGCGCA TGC^GCA
BamHI 3100 GGATCC G^GATCC
BbvCI 1127 CCTCAGC CCTCAGC(-5/-2)
BcgI 2539 CGATACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4374 TGATCA T^GATCA
BsaAI 2341 TACGTG [CT]AC^GT[GA]
BsmI 4944 GAATGC GAATGC(1/-1)
BstAPI 6691 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2957 TTCGAA TT^CGAA
DraIII 970 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1933 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 4374 TGATCA T^GATCA
FspI 3196 TGCGCA TGC^GCA
HpaI 3552 GTTAAC GTT^AAC
NspV 2957 TTCGAA TT^CGAA
Psp1406I 6050 AACGTT AA^CGTT
SapI 1264 GAAGAGC GCTCTTC(1/4)
SbfI 911 CCTGCAGG CCTGCA^GG
SfuI 2957 TTCGAA TT^CGAA
SgrAI 2003 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4219 ACTAGT A^CTAGT
Sse8387I 911 CCTGCAGG CCTGCA^GG
SwaI 3581 ATTTAAAT ATTT^AAAT
XbaI 4663 TCTAGA T^CTAGA
XcmI 1631 CCACCGACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2342 ACGTGT A^C[GA][CT]GT
4444 ACGTGT
Aor51HI 2240 AGCGCT AGC^GCT
2432 AGCGCT
Asp700I 3447 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4778 GAAATCATTC
AspI 647 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACCAGGTC
BbsI 2107 GTCTTC GAAGAC(2/6)
3594 GTCTTC
BglII 2336 AGATCT A^GATCT
6106 AGATCT
BlpI 1653 GCTGAGC GC^T[ACGT]AGC
2229 GCTCAGC
Bpu1102I 1653 GCTGAGC GC^T[ACGT]AGC
2229 GCTCAGC
BpuAI 2107 GTCTTC GAAGAC(2/6)
3594 GTCTTC
BsaWI 501 TCCGGT [AT]^CCGG[AT]
5721 TCCGGT
BstEII 583 GGTCACC G^GT[ACGT]ACC
2197 GGTCACC
BstPI 583 GGTCACC G^GT[ACGT]ACC
2197 GGTCACC
CelII 1653 GCTGAGC GC^T[ACGT]AGC
2229 GCTCAGC
Eco47III 2240 AGCGCT AGC^GCT
2432 AGCGCT
EcoO65I 583 GGTCACC G^GT[ACGT]ACC
2197 GGTCACC
EcoRV 94 GATATC GAT^ATC
5137 GATATC
MslI 832 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2937 CACGCTGGTG
NdeI 4747 CATATG CA^TATG
6444 CATATG
PflFI 647 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACCAGGTC
PshAI 268 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5476 GACAGCTGTC
SacI 245 GAGCTC GAGCT^C
1231 GAGCTC
SfiI 2074 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2599 GGCCCCACGGGCC
Tth111I 647 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACCAGGTC
XmnI 3447 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4778 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApaI ApoI AseI AsnI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BssSI Bst1107I BstF5I BstNI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI