##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AvrII BanII
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BstXI ClaI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I FauI FseI HgaI
Hin1I KasI KpnI KspI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SnaBI SpeI Sse8387I
Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 2 CGCG CG^CG
AclI 2710 AACGTT AA^CGTT
Alw44I 812 GTGCAC G^TGCAC
ApaLI 812 GTGCAC G^TGCAC
AspHI 812 GTGCAC G[AT]GC[AT]^C
AviII 1702 TGCGCA TGC^GCA
BamHI 1749 GGATCC G^GATCC
BciVI 1395 GGATAC GTATCC(6/5)
BlpI 1713 GCTGAGC GC^T[ACGT]AGC
BmyI 812 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1713 GCTGAGC GC^T[ACGT]AGC
BsgI 5703 CTGCAC GTGCAG(16/14)
BsiEI 8 CGACCG CG[GA][CT]^CG
BsiHKAI 812 GTGCAC G[AT]GC[AT]^C
Bsp1286I 812 GTGCAC G[AGT]GC[ACT]^C
BspMI 4697 ACCTGC ACCTGC(4/8)
BsrFI 1963 ACCGGC [GA]^CCGG[CT]
BssSI 937 CACGAG CACGAG(-5/-1)
BstBI 2779 TTCGAA TT^CGAA
BstUI 2 CGCG CG^CG
CelII 1713 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1963 ACCGGC [GA]^CCGG[CT]
DraII 147 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2205 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2360 GACTAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3082 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 147 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 943 GAATTC G^AATTC
EcoRV 1149 GATATC GAT^ATC
FspI 1702 TGCGCA TGC^GCA
HaeII 5590 AGCGCC [GA]GCGC^[CT]
HindIII 3146 AAGCTT A^AGCTT
MfeI 3836 CAATTG C^AATTG
MunI 3836 CAATTG C^AATTG
MvnI 2 CGCG CG^CG
NruI 1 TCGCGA TCG^CGA
NspV 2779 TTCGAA TT^CGAA
PpuMI 147 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2710 AACGTT AA^CGTT
SfuI 2779 TTCGAA TT^CGAA
SgrAI 1962 CACCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AocI 842 CCTAAGG CC^T[ACGT]AGG
6554 CCTTAGG
AvaII 148 GGACC G^G[AT]CC
246 GGACC
BalI 2730 TGGCCA TGG^CCA
4150 TGGCCA
BanI 459 GGTGCC G^G[CT][GA]CC
2432 GGTGCC
BbsI 59 GAAGAC GAAGAC(2/6)
5468 GTCTTC
BclI 722 TGATCA T^GATCA
1922 TGATCA
BpuAI 59 GAAGAC GAAGAC(2/6)
5468 GTCTTC
BsaI 1783 GGTCTC GGTCTC(1/5)
4437 GGTCTC
BseRI 1983 GAGGAG GAGGAG(10/8)
6031 GAGGAG
BsmFI 5077 GGGAC GGGAC(10/14)
1287 GTCCC
BspHI 1592 TCATGA T^CATGA
3000 TCATGA
BstEII 1403 GGTTACC G^GT[ACGT]ACC
5252 GGTTACC
BstPI 1403 GGTTACC G^GT[ACGT]ACC
5252 GGTTACC
Bsu36I 842 CCTAAGG CC^T[ACGT]AGG
6554 CCTTAGG
EaeI 2730 TGGCCA [CT]^GGCC[GA]
4150 TGGCCA
EarI 580 CTCTTC CTCTTC(1/4)
3722 CTCTTC
Eco81I 842 CCTAAGG CC^T[ACGT]AGG
6554 CCTTAGG
EcoO65I 1403 GGTTACC G^GT[ACGT]ACC
5252 GGTTACC
FbaI 722 TGATCA T^GATCA
1922 TGATCA
HpaI 176 GTTAAC GTT^AAC
3028 GTTAAC
Ksp632I 580 CTCTTC CTCTTC(1/4)
3722 CTCTTC
MaeII 2711 ACGT A^CGT
4055 ACGT
MluNI 2730 TGGCCA TGG^CCA
4150 TGGCCA
MscI 2730 TGGCCA TGG^CCA
4150 TGGCCA
MspA1I 1138 CAGCTG C[AC]G^C[GT]G
1492 CAGCTG
PstI 4098 CTGCAG CTGCA^G
6564 CTGCAG
PvuII 1138 CAGCTG CAG^CTG
1492 CAGCTG
RcaI 1592 TCATGA T^CATGA
3000 TCATGA
ScaI 3007 AGTACT AGT^ACT
4079 AGTACT
SmaI 52 CCCGGG CCC^GGG
160 CCCGGG
TaqI 2557 TCGA T^CGA
2780 TCGA
TthHB8I 2557 TCGA T^CGA
2780 TCGA
XbaI 1130 TCTAGA T^CTAGA
6544 TCTAGA
XmaCI 52 CCCGGG C^CCGGG
160 CCCGGG
XmaI 52 CCCGGG C^CCGGG
160 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaI BbvI BcnI
BfaI BglII BpmI BsaJI BsiYI
BslI BsmAI BsmI BsoBI Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstYI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI SspI StuI StyI SwaI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI