##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AgeI Aor51HI AscI BbeI
BbrPI BsaAI BsiEI BsiWI BssHII
Bst1107I BstZ17I DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HaeII HindIII KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI SacII
SbfI SexAI SfoI SgrAI SnaBI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 6737 GGTACC G^GTACC
AccIII 3220 TCCGGA T^CCGGA
AflII 6321 CTTAAG C^TTAAG
AocI 6800 CCTCAGG CC^T[ACGT]AGG
ApaI 2140 GGGCCC GGGCC^C
AseI 3099 ATTAAT AT^TAAT
AsnI 3099 ATTAAT AT^TAAT
Asp718I 6737 GGTACC G^GTACC
AviII 3175 TGCGCA TGC^GCA
BcgI 1552 CGAAAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4476 GTATCC GTATCC(6/5)
BfrI 6321 CTTAAG C^TTAAG
BglI 2631 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3220 TCCGGA [AT]^CCGG[AT]
BseAI 3220 TCCGGA T^CCGGA
BsmBI 1548 GAGACG CGTCTC(1/5)
BspDI 3666 ATCGAT AT^CGAT
BspEI 3220 TCCGGA T^CCGGA
BspLU11I 1771 ACATGT A^CATGT
BssSI 3969 CTCGTG CACGAG(-5/-1)
Bsu36I 6800 CCTCAGG CC^T[ACGT]AGG
ClaI 3666 ATCGAT AT^CGAT
CpoI 3741 CGGTCCG CG^G[AT]CCG
Eco81I 6800 CCTCAGG CC^T[ACGT]AGG
FspI 3175 TGCGCA TGC^GCA
HpaI 5557 GTTAAC GTT^AAC
KpnI 6737 GGTACC GGTAC^C
MfeI 3533 CAATTG C^AATTG
MroI 3220 TCCGGA T^CCGGA
MunI 3533 CAATTG C^AATTG
PshAI 3434 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3099 ATTAAT AT^TAAT
RsrII 3741 CGGTCCG CG^G[AT]CCG
SacI 4823 GAGCTC GAGCT^C
SalI 4250 GTCGAC G^TCGAC
SfiI 2630 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2206 CCCGGG CCC^GGG
SpeI 2885 ACTAGT A^CTAGT
SphI 3904 GCATGC GCATG^C
XmaCI 2206 CCCGGG C^CCGGG
XmaI 2206 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 82 GACGTC GACGT^C
2519 GACGTC
AccI 3033 GTCTAC GT^[AC][GT]AC
4250 GTCGAC
AccII 3813 CGCG CG^CG
3994 CGCG
AcyI 82 GACGTC G[GA]^CG[CT]C
2519 GACGTC
AhdI 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7105 GACCAACCGTC
AspEI 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7105 GACCAACCGTC
AvrII 593 CCTAGG C^CTAGG
7158 CCTAGG
BglII 653 AGATCT A^GATCT
6807 AGATCT
BlnI 593 CCTAGG C^CTAGG
7158 CCTAGG
BlpI 2055 GCTAAGC GC^T[ACGT]AGC
2178 GCTCAGC
Bpu1102I 2055 GCTAAGC GC^T[ACGT]AGC
2178 GCTCAGC
BsaBI 1640 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1808 GATCCCCATC
BsaHI 82 GACGTC G[GA]^CG[CT]C
2519 GACGTC
Bsp1407I 4486 TGTACA T^GTACA
5076 TGTACA
BspHI 1060 TCATGA T^CATGA
3817 TCATGA
BsrBI 3758 CCGCTC CCGCTC(-3/-3)
1602 GAGCGG
BsrGI 4486 TGTACA T^GTACA
5076 TGTACA
BstBI 1129 TTCGAA TT^CGAA
1359 TTCGAA
BstEII 2486 GGTCACC G^GT[ACGT]ACC
7187 GGTCACC
BstPI 2486 GGTCACC G^GT[ACGT]ACC
7187 GGTCACC
BstUI 3813 CGCG CG^CG
3994 CGCG
CelII 2055 GCTAAGC GC^T[ACGT]AGC
2178 GCTCAGC
DraI 5839 TTTAAA TTT^AAA
5864 TTTAAA
Eam1105I 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7105 GACCAACCGTC
EcoO65I 2486 GGTCACC G^GT[ACGT]ACC
7187 GGTCACC
HgaI 3814 GCGTC GACGC(5/10)
4501 GCGTC
Hin1I 82 GACGTC G[GA]^CG[CT]C
2519 GACGTC
MamI 1640 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1808 GATCCCCATC
MvnI 3813 CGCG CG^CG
3994 CGCG
NcoI 1000 CCATGG C^CATGG
5190 CCATGG
NspV 1129 TTCGAA TT^CGAA
1359 TTCGAA
RcaI 1060 TCATGA T^CATGA
3817 TCATGA
ScaI 384 AGTACT AGT^ACT
4862 AGTACT
SfuI 1129 TTCGAA TT^CGAA
1359 TTCGAA
SspBI 4486 TGTACA T^GTACA
5076 TGTACA
StuI 2808 AGGCCT AGG^CCT
4734 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII XmnI