##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AgeI Aor51HI AscI AspI
BamHI BbrPI BsiEI BsiWI BspDI
BssHII BstBI BstEII BstPI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
HpaI MluI NaeI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SphI StuI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 3249 GGTACC G^GTACC
AccIII 2582 TCCGGA T^CCGGA
AclI 4383 AACGTT AA^CGTT
AcyI 4000 GGCGCC G[GA]^CG[CT]C
AflIII 6271 ACATGT A^C[GA][CT]GT
AhdI 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4105 GGGCCC GGGCC^C
Asp718I 3249 GGTACC G^GTACC
AspEI 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3511 CCCGAG C^[CT]CG[GA]G
AviII 3946 TGCGCA TGC^GCA
AvrII 1517 CCTAGG C^CTAGG
BalI 991 TGGCCA TGG^CCA
BbeI 4000 GGCGCC GGCGC^C
BcgI 739 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 898 TGATCA T^GATCA
BlnI 1517 CCTAGG C^CTAGG
BlpI 1057 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1057 GCTCAGC GC^T[ACGT]AGC
BsaBI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4000 GGCGCC G[GA]^CG[CT]C
BseAI 2582 TCCGGA T^CCGGA
BsmBI 3610 GAGACG CGTCTC(1/5)
BsoBI 3511 CCCGAG C^[CT]CG[GA]G
Bsp1407I 5753 TGTACA T^GTACA
BspEI 2582 TCCGGA T^CCGGA
BspLU11I 6271 ACATGT A^CATGT
BsrDI 1062 GCAATG GCAATG(2/0)
BsrGI 5753 TGTACA T^GTACA
BssSI 3205 CTCGTG CACGAG(-5/-1)
Bst1107I 4671 GTATAC GTA^TAC
BstZ17I 4671 GTATAC GTA^TAC
CelII 1057 GCTCAGC GC^T[ACGT]AGC
Eam1105I 5218 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 898 TGATCA T^GATCA
FspI 3946 TGCGCA TGC^GCA
HaeII 4000 GGCGCC [GA]GCGC^[CT]
Hin1I 4000 GGCGCC G[GA]^CG[CT]C
KasI 4000 GGCGCC G^GCGCC
KpnI 3249 GGTACC GGTAC^C
KspI 4128 CCGCGG CCGC^GG
MamI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 991 TGGCCA TGG^CCA
MroI 2582 TCCGGA T^CCGGA
MscI 991 TGGCCA TGG^CCA
NarI 4000 GGCGCC GG^CGCC
PflMI 1761 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4757 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4383 AACGTT AA^CGTT
SacII 4128 CCGCGG CCGC^GG
SapI 1742 GCTCTTC GCTCTTC(1/4)
SbfI 764 CCTGCAGG CCTGCA^GG
SfoI 4000 GGCGCC GGC^GCC
SnaBI 5732 TACGTA TAC^GTA
SpeI 6110 ACTAGT A^CTAGT
Sse8387I 764 CCTGCAGG CCTGCA^GG
SspBI 5753 TGTACA T^GTACA
SwaI 6033 ATTTAAAT ATTT^AAAT
Van91I 1761 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5293 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 907 GTGCAC G^TGCAC
4564 GTGCAC
AlwI 4084 GATCC GGATC(4/5)
418 GGATC
AocI 2090 CCTCAGG CC^T[ACGT]AGG
3853 CCTCAGG
ApaLI 907 GTGCAC G^TGCAC
4564 GTGCAC
BbvCI 1044 CCTCAGC CCTCAGC(-5/-2)
1013 GCTGAGG
BglI 30 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3698 GCCTGCCGGGC
BsaWI 415 ACCGGA [AT]^CCGG[AT]
2582 TCCGGA
BspHI 1606 TCATGA T^CATGA
1622 TCATGA
Bsu36I 2090 CCTCAGG CC^T[ACGT]AGG
3853 CCTCAGG
DraIII 3625 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5945 CACTTCGTG
EaeI 991 TGGCCA [CT]^GGCC[GA]
3055 TGGCCG
EarI 1743 CTCTTC CTCTTC(1/4)
537 GAAGAG
Eco81I 2090 CCTCAGG CC^T[ACGT]AGG
3853 CCTCAGG
EcoRI 2676 GAATTC G^AATTC
5026 GAATTC
EcoT14I 11 CCTTGG C^C[AT][AT]GG
1517 CCTAGG
HgaI 3268 GACGC GACGC(5/10)
3979 GCGTC
Ksp632I 1743 CTCTTC CTCTTC(1/4)
537 GAAGAG
MfeI 2797 CAATTG C^AATTG
5401 CAATTG
MunI 2797 CAATTG C^AATTG
5401 CAATTG
NdeI 5757 CATATG CA^TATG
5871 CATATG
PpuMI 892 AGGACCT [GA]G^G[AT]CC[CT]
2560 AGGTCCT
PvuII 2826 CAGCTG CAG^CTG
3289 CAGCTG
RcaI 1606 TCATGA T^CATGA
1622 TCATGA
SacI 647 GAGCTC GAGCT^C
1740 GAGCTC
StyI 11 CCTTGG C^C[AT][AT]GG
1517 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EcoO109I EcoRII
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI