##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
ApoI AscI AseI AsnI Asp700I
AspEI AspI BamHI BbrPI BbvCI
BfrI BsiEI BsiWI BsmI BssSI
Bst1107I BstBI BstZ17I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I HindIII HpaI
NdeI NheI NotI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SbfI ScaI SfiI SfuI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 4952 GTCGAC GT^[AC][GT]AC
AccIII 274 TCCGGA T^CCGGA
Alw44I 3516 GTGCAC G^TGCAC
AocI 3536 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4889 AGCGCT AGC^GCT
ApaLI 3516 GTGCAC G^TGCAC
AvrII 4876 CCTAGG C^CTAGG
BciVI 3310 GTATCC GTATCC(6/5)
BclI 1506 TGATCA T^GATCA
BlnI 4876 CCTAGG C^CTAGG
BsaAI 2824 CACGTA [CT]AC^GT[GA]
BsaBI 266 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 274 TCCGGA T^CCGGA
BspDI 2359 ATCGAT AT^CGAT
BspEI 274 TCCGGA T^CCGGA
BspHI 1955 TCATGA T^CATGA
BspLU11I 516 ACATGT A^CATGT
BstEII 3467 GGTAACC G^GT[ACGT]ACC
BstPI 3467 GGTAACC G^GT[ACGT]ACC
Bsu36I 3536 CCTCAGG CC^T[ACGT]AGG
ClaI 2359 ATCGAT AT^CGAT
DraI 5211 TTTAAA TTT^AAA
Eco47III 4889 AGCGCT AGC^GCT
Eco81I 3536 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3467 GGTAACC G^GT[ACGT]ACC
FbaI 1506 TGATCA T^GATCA
FseI 5011 GGCCGGCC GGCCGG^CC
KspI 142 CCGCGG CCGC^GG
MamI 266 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4237 CAATTG C^AATTG
MluI 4644 ACGCGT A^CGCGT
MroI 274 TCCGGA T^CCGGA
MunI 4237 CAATTG C^AATTG
NruI 88 TCGCGA TCG^CGA
RcaI 1955 TCATGA T^CATGA
SacII 142 CCGCGG CCGC^GG
SalI 4952 GTCGAC G^TCGAC
SexAI 2291 ACCTGGT A^CC[AT]GGT
SgrAI 408 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2186 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AviII 1364 TGCGCA TGC^GCA
1916 TGCGCA
BglII 832 AGATCT A^GATCT
1382 AGATCT
BsaWI 274 TCCGGA [AT]^CCGG[AT]
1606 TCCGGT
BsmBI 768 CGTCTC CGTCTC(1/5)
746 GAGACG
Bsp1407I 1835 TGTACA T^GTACA
5443 TGTACA
BsrGI 1835 TGTACA T^GTACA
5443 TGTACA
BstAPI 284 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2177 GCAAGAGCTGC
FspI 1364 TGCGCA TGC^GCA
1916 TGCGCA
HincII 991 GTCAAC GT[CT]^[GA]AC
4952 GTCGAC
HindII 991 GTCAAC GT[CT]^[GA]AC
4952 GTCGAC
NaeI 409 GCCGGC GCC^GGC
5012 GCCGGC
NgoMIV 409 GCCGGC G^CCGGC
5012 GCCGGC
PaeR7I 1900 CTCGAG C^TCGAG
3113 CTCGAG
PshAI 2422 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3369 GACACAGGTC
SspBI 1835 TGTACA T^GTACA
5443 TGTACA
XbaI 3862 TCTAGA T^CTAGA
4155 TCTAGA
XhoI 1900 CTCGAG C^TCGAG
3113 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII Acc65I AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI