##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AhdI Alw44I ApaLI AscI
AspEI AviII AvrII BalI BbeI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRV FseI FspI HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NheI NotI
NruI PmeI PpuMI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SspBI SwaI XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 3355 GGTACC G^GTACC
AccII 2851 CGCG CG^CG
AgeI 2178 ACCGGT A^CCGGT
Aor51HI 667 AGCGCT AGC^GCT
ApaI 2162 GGGCCC GGGCC^C
Asp718I 3355 GGTACC G^GTACC
AspI 225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 679 CTCGAG C^[CT]CG[GA]G
BamHI 1824 GGATCC G^GATCC
BbrPI 549 CACGTG CAC^GTG
BglII 1940 AGATCT A^GATCT
BsaI 1540 GAGACC GGTCTC(1/5)
BsaWI 2178 ACCGGT [AT]^CCGG[AT]
BsgI 55 CTGCAC GTGCAG(16/14)
BsmFI 1386 GGGAC GGGAC(10/14)
BsmI 2479 GCATTC GAATGC(1/-1)
BsoBI 679 CTCGAG C^[CT]CG[GA]G
BspLU11I 2902 ACATGT A^CATGT
BstBI 2721 TTCGAA TT^CGAA
BstUI 2851 CGCG CG^CG
CfoI 668 GCGC GCG^C
DrdI 2063 GACGAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2024 TGGCCG [CT]^GGCC[GA]
Eco47III 667 AGCGCT AGC^GCT
EcoRI 2972 GAATTC G^AATTC
FauI 2165 CCCGC CCCGC(4/6)
HaeII 667 AGCGCT [GA]GCGC^[CT]
HhaI 668 GCGC GCG^C
HinP1I 668 GCGC G^CGC
HincII 2741 GTTGAC GT[CT]^[GA]AC
HindII 2741 GTTGAC GT[CT]^[GA]AC
HindIII 273 AAGCTT A^AGCTT
KpnI 3355 GGTACC GGTAC^C
MspA1I 1953 CAGCTG C[AC]G^C[GT]G
MvnI 2851 CGCG CG^CG
NaeI 2026 GCCGGC GCC^GGC
NdeI 2286 CATATG CA^TATG
NgoMIV 2026 GCCGGC G^CCGGC
NspV 2721 TTCGAA TT^CGAA
PacI 3086 TTAATTAA TTAAT^TAA
PaeR7I 679 CTCGAG C^TCGAG
PflFI 225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2178 ACCGGT A^CCGGT
PmaCI 549 CACGTG CAC^GTG
PmlI 549 CACGTG CAC^GTG
PshAI 1898 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1953 CAGCTG CAG^CTG
SacI 3373 GAGCTC GAGCT^C
SbfI 1097 CCTGCAGG CCTGCA^GG
ScaI 3541 AGTACT AGT^ACT
SfuI 2721 TTCGAA TT^CGAA
Sse8387I 1097 CCTGCAGG CCTGCA^GG
SspI 3607 AATATT AAT^ATT
Tth111I 225 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 679 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 264 CCTGAGG CC^T[ACGT]AGG
981 CCTGAGG
BanI 156 GGTGCC G^G[CT][GA]CC
3355 GGTACC
BanII 2162 GGGCCC G[GA]GC[CT]^C
3373 GAGCTC
BbsI 17 GAAGAC GAAGAC(2/6)
259 GAAGAC
BbvI 1028 GCAGC GCAGC(8/12)
1955 GCTGC
BclI 440 TGATCA T^GATCA
2640 TGATCA
BpuAI 17 GAAGAC GAAGAC(2/6)
259 GAAGAC
BsaAI 194 TACGTG [CT]AC^GT[GA]
549 CACGTG
BsrFI 2026 GCCGGC [GA]^CCGG[CT]
2178 ACCGGT
BstAPI 589 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
950 GCAGACTCTGC
BstXI 1579 CCATTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1984 CCATGGAGATGG
BstYI 1824 GGATCC [GA]^GATC[CT]
1940 AGATCT
Bsu36I 264 CCTGAGG CC^T[ACGT]AGG
981 CCTGAGG
Cfr10I 2026 GCCGGC [GA]^CCGG[CT]
2178 ACCGGT
DraI 3006 TTTAAA TTT^AAA
3522 TTTAAA
DraII 628 AGGGCCT [GA]G^G[ACGT]CC[CT]
811 AGGGCCT
EarI 2512 CTCTTC CTCTTC(1/4)
2792 CTCTTC
Eco81I 264 CCTGAGG CC^T[ACGT]AGG
981 CCTGAGG
EcoO109I 628 AGGGCCT [GA]G^G[ACGT]CC[CT]
811 AGGGCCT
FbaI 440 TGATCA T^GATCA
2640 TGATCA
HapII 2027 CCGG C^CGG
2179 CCGG
HpaII 2027 CCGG C^CGG
2179 CCGG
Ksp632I 2512 CTCTTC CTCTTC(1/4)
2792 CTCTTC
MflI 1824 GGATCC [GA]^GATC[CT]
1940 AGATCT
MspI 2027 CCGG C^CGG
2179 CCGG
PflMI 1828 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1952 CCAGCTGCTGG
PstI 948 CTGCAG CTGCA^G
1098 CTGCAG
StuI 1094 AGGCCT AGG^CCT
2009 AGGCCT
TseI 1028 GCAGC G^C[AT]GC
1955 GCTGC
Van91I 1828 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1952 CCAGCTGCTGG
XhoII 1824 GGATCC [GA]^GATC[CT]
1940 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XmnI