##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII BalI BbeI
BbrPI BcgI BglI BlpI BpmI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HaeII Hin1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 1343 CTTAAG C^TTAAG
AflIII 2087 ACATGT A^C[GA][CT]GT
AocI 472 CCTCAGG CC^T[ACGT]AGG
AseI 3818 ATTAAT AT^TAAT
AsnI 3818 ATTAAT AT^TAAT
AspHI 1395 GAGCTC G[AT]GC[AT]^C
AvrII 2464 CCTAGG C^CTAGG
BamHI 1762 GGATCC G^GATCC
BanI 2604 GGTGCC G^G[CT][GA]CC
BanII 1395 GAGCTC G[GA]GC[CT]^C
BclI 1705 TGATCA T^GATCA
BcnI 1065 CCCGG CC^[GC]GG
BfrI 1343 CTTAAG C^TTAAG
BlnI 2464 CCTAGG C^CTAGG
BmyI 1395 GAGCTC G[AGT]GC[ACT]^C
BsaAI 3873 TACGTG [CT]AC^GT[GA]
BsgI 2893 CTGCAC GTGCAG(16/14)
BsiHKAI 1395 GAGCTC G[AT]GC[AT]^C
BsmI 415 GCATTC GAATGC(1/-1)
Bsp1286I 1395 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 2087 ACATGT A^CATGT
BsrDI 625 CATTGC GCAATG(2/0)
BstBI 279 TTCGAA TT^CGAA
Bsu36I 472 CCTCAGG CC^T[ACGT]AGG
CfoI 3680 GCGC GCG^C
DraII 1908 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 472 CCTCAGG CC^T[ACGT]AGG
EcoNI 956 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1908 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 949 CCCGC CCCGC(4/6)
FbaI 1705 TGATCA T^GATCA
HapII 1066 CCGG C^CGG
HgaI 1818 GCGTC GACGC(5/10)
HhaI 3680 GCGC GCG^C
HinP1I 3680 GCGC G^CGC
HpaI 1481 GTTAAC GTT^AAC
HpaII 1066 CCGG C^CGG
MslI 1111 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1066 CCGG C^CGG
NciI 1065 CCCGG CC^[GC]GG
NdeI 2856 CATATG CA^TATG
NspV 279 TTCGAA TT^CGAA
PflMI 3392 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3818 ATTAAT AT^TAAT
SacI 1395 GAGCTC GAGCT^C
SalI 153 GTCGAC G^TCGAC
ScaI 2031 AGTACT AGT^ACT
SfuI 279 TTCGAA TT^CGAA
SpeI 3773 ACTAGT A^CTAGT
Van91I 3392 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3709 TCTAGA T^CTAGA
XcmI 1326 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 153 GTCGAC GT^[AC][GT]AC
2083 GTAGAC
AlwNI 114 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1446 CAGAACCTG
BbsI 506 GTCTTC GAAGAC(2/6)
3402 GTCTTC
BbvCI 1810 CCTCAGC CCTCAGC(-5/-2)
2576 GCTGAGG
BpuAI 506 GTCTTC GAAGAC(2/6)
3402 GTCTTC
BsaBI 431 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3647 GATTGAAATC
BsaI 940 GAGACC GGTCTC(1/5)
1493 GAGACC
BseRI 1808 CTCCTC GAGGAG(10/8)
174 GAGGAG
BstXI 247 CCATGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
392 CCAGTCAACTGG
EcoRI 788 GAATTC G^AATTC
870 GAATTC
EcoRV 662 GATATC GAT^ATC
797 GATATC
MamI 431 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3647 GATTGAAATC
TaqI 154 TCGA T^CGA
280 TCGA
TthHB8I 154 TCGA T^CGA
280 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BciVI
BfaI BglII BsaJI BsiYI BslI
BsmAI BsmFI Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI