##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI BbeI BbsI
BclI BfrI BglI BpuAI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 416 GTCTAC GT^[AC][GT]AC
AhdI 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3867 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3177 TGCGCA TGC^GCA
AvrII 4274 CCTAGG C^CTAGG
BamHI 2725 GGATCC G^GATCC
BciVI 1374 GTATCC GTATCC(6/5)
BlnI 4274 CCTAGG C^CTAGG
BlpI 3013 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3013 GCTCAGC GC^T[ACGT]AGC
BsaBI 364 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1054 CGATCG CG[GA][CT]^CG
Bsp1407I 4111 TGTACA T^GTACA
BspHI 2037 TCATGA T^CATGA
BsrFI 3212 GCCGGC [GA]^CCGG[CT]
BsrGI 4111 TGTACA T^GTACA
BstEII 3041 GGTCACC G^GT[ACGT]ACC
BstPI 3041 GGTCACC G^GT[ACGT]ACC
CelII 3013 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3212 GCCGGC [GA]^CCGG[CT]
DrdI 695 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2117 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3041 GGTCACC G^GT[ACGT]ACC
EcoT22I 2214 ATGCAT ATGCA^T
FspI 3177 TGCGCA TGC^GCA
HindIII 3868 AAGCTT A^AGCTT
MamI 364 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 232 TTAA T^TAA
NaeI 3212 GCCGGC GCC^GGC
NgoMIV 3212 GCCGGC G^CCGGC
NsiI 2214 ATGCAT ATGCA^T
PaeR7I 1934 CTCGAG C^TCGAG
PflMI 1343 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3902 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1054 CGATCG CGAT^CG
RcaI 2037 TCATGA T^CATGA
SacI 2783 GAGCTC GAGCT^C
SmaI 4105 CCCGGG CCC^GGG
SspBI 4111 TGTACA T^GTACA
Tru9I 232 TTAA T^TAA
Van91I 1343 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 201 TCTAGA T^CTAGA
XhoI 1934 CTCGAG C^TCGAG
XmaCI 4105 CCCGGG C^CCGGG
XmaI 4105 CCCGGG C^CCGGG
XmnI 3867 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 2192 CGCG CG^CG
3200 CGCG
Alw44I 1574 GTGCAC G^TGCAC
2678 GTGCAC
AocI 2851 CCTGAGG CC^T[ACGT]AGG
4027 CCTGAGG
ApaLI 1574 GTGCAC G^TGCAC
2678 GTGCAC
BalI 1446 TGGCCA TGG^CCA
4069 TGGCCA
BbvCI 3893 CCTCAGC CCTCAGC(-5/-2)
1268 GCTGAGG
BcgI 1562 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1968 GCATCTCCTTCG
BsrBI 1478 GAGCGG CCGCTC(-3/-3)
2732 GAGCGG
BsrDI 2946 GCAATG GCAATG(2/0)
3781 GCAATG
BstUI 2192 CGCG CG^CG
3200 CGCG
Bsu36I 2851 CCTGAGG CC^T[ACGT]AGG
4027 CCTGAGG
DraIII 1341 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2606 CACCAGGTG
Eco81I 2851 CCTGAGG CC^T[ACGT]AGG
4027 CCTGAGG
EcoRI 2951 GAATTC G^AATTC
3474 GAATTC
HaeII 1483 GGCGCT [GA]GCGC^[CT]
3110 AGCGCC
HincII 565 GTCAAC GT[CT]^[GA]AC
977 GTCAAC
HindII 565 GTCAAC GT[CT]^[GA]AC
977 GTCAAC
MluNI 1446 TGGCCA TGG^CCA
4069 TGGCCA
MscI 1446 TGGCCA TGG^CCA
4069 TGGCCA
MvnI 2192 CGCG CG^CG
3200 CGCG
StuI 903 AGGCCT AGG^CCT
3445 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbvI BcnI BfaI BglII BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII