##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AocI ApaI AscI
Asp718I AspEI BbeI BbrPI BcgI
BfrI BsaAI BsaHI BsiEI BsiWI
BsmBI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I CpoI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I FseI Hin1I
HpaI KasI KpnI KspI MluI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 3815 CGCG CG^CG
Alw44I 2882 GTGCAC G^TGCAC
Aor51HI 2786 AGCGCT AGC^GCT
ApaLI 2882 GTGCAC G^TGCAC
AseI 1212 ATTAAT AT^TAAT
AsnI 1212 ATTAAT AT^TAAT
AspI 2047 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4371 TGCGCA TGC^GCA
BalI 10 TGGCCA TGG^CCA
BamHI 4130 GGATCC G^GATCC
BlpI 2789 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2789 GCTCAGC GC^T[ACGT]AGC
BsmI 2433 GCATTC GAATGC(1/-1)
Bsp1407I 2202 TGTACA T^GTACA
BspDI 2722 ATCGAT AT^CGAT
BsrGI 2202 TGTACA T^GTACA
BssSI 4325 CACGAG CACGAG(-5/-1)
BstUI 3815 CGCG CG^CG
CelII 2789 GCTCAGC GC^T[ACGT]AGC
ClaI 2722 ATCGAT AT^CGAT
DraIII 2944 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4673 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2786 AGCGCT AGC^GCT
EcoRV 3715 GATATC GAT^ATC
FauI 52 CCCGC CCCGC(4/6)
FspI 4371 TGCGCA TGC^GCA
MluNI 10 TGGCCA TGG^CCA
MscI 10 TGGCCA TGG^CCA
MvnI 3815 CGCG CG^CG
NheI 636 GCTAGC G^CTAGC
PaeR7I 3761 CTCGAG C^TCGAG
PflFI 2047 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1212 ATTAAT AT^TAAT
SacI 1324 GAGCTC GAGCT^C
SapI 1084 GAAGAGC GCTCTTC(1/4)
SspBI 2202 TGTACA T^GTACA
SwaI 5336 ATTTAAAT ATTT^AAAT
Tth111I 2047 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3761 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 3035 TCCGGA T^CCGGA
4127 TCCGGA
Asp700I 3836 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4578 GAAAGGCTTC
AvrII 822 CCTAGG C^CTAGG
3589 CCTAGG
BanI 1609 GGTGCC G^G[CT][GA]CC
4267 GGTGCC
BanII 183 GAGCCC G[GA]GC[CT]^C
1324 GAGCTC
BbvCI 2977 GCTGAGG CCTCAGC(-5/-2)
3687 GCTGAGG
BclI 657 TGATCA T^GATCA
3048 TGATCA
BglI 2626 GCCCTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4718 GCCGCCGAGGC
BlnI 822 CCTAGG C^CTAGG
3589 CCTAGG
BpmI 1243 CTGGAG CTGGAG(16/14)
1276 CTGGAG
BsaBI 3174 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4565 GATATTTATC
BseAI 3035 TCCGGA T^CCGGA
4127 TCCGGA
BspEI 3035 TCCGGA T^CCGGA
4127 TCCGGA
BspHI 3065 TCATGA T^CATGA
5263 TCATGA
BspLU11I 662 ACATGT A^CATGT
5564 ACATGT
EcoT22I 3962 ATGCAT ATGCA^T
4733 ATGCAT
FbaI 657 TGATCA T^GATCA
3048 TGATCA
HaeII 2786 AGCGCT [GA]GCGC^[CT]
4330 GGCGCT
HgaI 1680 GCGTC GACGC(5/10)
2925 GCGTC
HincII 4455 GTTGAC GT[CT]^[GA]AC
4473 GTCAAC
HindII 4455 GTTGAC GT[CT]^[GA]AC
4473 GTCAAC
MamI 3174 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4565 GATATTTATC
MfeI 1046 CAATTG C^AATTG
4917 CAATTG
MroI 3035 TCCGGA T^CCGGA
4127 TCCGGA
MunI 1046 CAATTG C^AATTG
4917 CAATTG
NsiI 3962 ATGCAT ATGCA^T
4733 ATGCAT
PpuMI 707 GGGACCT [GA]G^G[AT]CC[CT]
4391 AGGTCCC
RcaI 3065 TCATGA T^CATGA
5263 TCATGA
ScaI 4141 AGTACT AGT^ACT
5443 AGTACT
StuI 1109 AGGCCT AGG^CCT
2735 AGGCCT
XmnI 3836 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4578 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII