##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI AspI
AviII AvrII BbeI BbrPI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspMI BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HaeII HgaI
Hin1I KasI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI Tth111I
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1823 GGTACC G^GTACC
AccII 255 CGCG CG^CG
AflIII 4176 ACATGT A^C[GA][CT]GT
AlwNI 1598 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 248 GGGCCC GGGCC^C
AseI 1561 ATTAAT AT^TAAT
AsnI 1561 ATTAAT AT^TAAT
Asp718I 1823 GGTACC G^GTACC
BalI 1358 TGGCCA TGG^CCA
BanI 1823 GGTACC G^G[CT][GA]CC
BanII 248 GGGCCC G[GA]GC[CT]^C
BbsI 1293 GTCTTC GAAGAC(2/6)
BbvCI 2420 CCTCAGC CCTCAGC(-5/-2)
BcgI 3061 CGAATAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1414 GTATCC GTATCC(6/5)
BglII 3039 AGATCT A^GATCT
BlpI 3295 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3295 GCTTAGC GC^T[ACGT]AGC
BpuAI 1293 GTCTTC GAAGAC(2/6)
BsaI 983 GAGACC GGTCTC(1/5)
BsmI 2099 GCATTC GAATGC(1/-1)
BspHI 3502 TCATGA T^CATGA
BspLU11I 4176 ACATGT A^CATGT
BsrBI 244 GAGCGG CCGCTC(-3/-3)
Bst1107I 1202 GTATAC GTA^TAC
BstAPI 3609 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 255 CGCG CG^CG
BstZ17I 1202 GTATAC GTA^TAC
CelII 3295 GCTTAGC GC^T[ACGT]AGC
DraIII 3610 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1358 TGGCCA [CT]^GGCC[GA]
HincII 490 GTTAAC GT[CT]^[GA]AC
HindII 490 GTTAAC GT[CT]^[GA]AC
HpaI 490 GTTAAC GTT^AAC
KpnI 1823 GGTACC GGTAC^C
KspI 254 CCGCGG CCGC^GG
MaeII 553 ACGT A^CGT
MluNI 1358 TGGCCA TGG^CCA
MscI 1358 TGGCCA TGG^CCA
MvnI 255 CGCG CG^CG
NcoI 1137 CCATGG C^CATGG
PaeR7I 970 CTCGAG C^TCGAG
PmeI 3410 GTTTAAAC GTTT^AAAC
PshAI 442 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1561 ATTAAT AT^TAAT
RcaI 3502 TCATGA T^CATGA
SacII 254 CCGCGG CCGC^GG
ScaI 3594 AGTACT AGT^ACT
SmaI 1806 CCCGGG CCC^GGG
SwaI 3633 ATTTAAAT ATTT^AAAT
XbaI 2376 TCTAGA T^CTAGA
XcmI 1077 CCAGGGGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 970 CTCGAG C^TCGAG
XmaCI 1806 CCCGGG C^CCGGG
XmaI 1806 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 3607 GTGCAC G^TGCAC
3616 GTGCAC
AocI 697 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
ApaLI 3607 GTGCAC G^TGCAC
3616 GTGCAC
Asp700I 1426 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2408 GAACATTTTC
AvaI 970 CTCGAG C^[CT]CG[GA]G
1806 CCCGGG
BamHI 498 GGATCC G^GATCC
3460 GGATCC
BclI 3499 TGATCA T^GATCA
4018 TGATCA
BcnI 1806 CCCGG CC^[GC]GG
1807 CCGGG
BsmFI 2039 GGGAC GGGAC(10/14)
2154 GTCCC
BsoBI 970 CTCGAG C^[CT]CG[GA]G
1806 CCCGGG
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
CfoI 991 GCGC GCG^C
1054 GCGC
DraII 248 GGGCCCC [GA]G^G[ACGT]CC[CT]
2395 AGGGCCT
DsaI 254 CCGCGG C^C[GA][CT]GG
1137 CCATGG
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
2017 CCTGAGG
EcoO109I 248 GGGCCCC [GA]G^G[ACGT]CC[CT]
2395 AGGGCCT
EcoRV 151 GATATC GAT^ATC
1849 GATATC
EcoT14I 444 CCTTGG C^C[AT][AT]GG
1137 CCATGG
FauI 253 CCCGC CCCGC(4/6)
246 GCGGG
FbaI 3499 TGATCA T^GATCA
4018 TGATCA
HapII 1807 CCGG C^CGG
2546 CCGG
HhaI 991 GCGC GCG^C
1054 GCGC
HinP1I 991 GCGC G^CGC
1054 GCGC
HpaII 1807 CCGG C^CGG
2546 CCGG
MspI 1807 CCGG C^CGG
2546 CCGG
NciI 1806 CCCGG CC^[GC]GG
1807 CCGGG
NspI 3918 GCATGT [GA]CATG^[CT]
4176 ACATGT
PflMI 1735 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2681 CCAATGAGTGG
PstI 528 CTGCAG CTGCA^G
3880 CTGCAG
SspI 37 AATATT AAT^ATT
1084 AATATT
StyI 444 CCTTGG C^C[AT][AT]GG
1137 CCATGG
TaqI 971 TCGA T^CGA
1188 TCGA
Tsp45I 440 GTGAC ^GT[GC]AC
2203 GTGAC
TthHB8I 971 TCGA T^CGA
1188 TCGA
Van91I 1735 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2681 CCAATGAGTGG
XmnI 1426 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2408 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AspHI AvaII
BbvI BfaI BfrI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI TfiI Tru9I TseI
Tsp509I TspRI XhoII