##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AcsI ApoI AscI
AseI AsnI Asp700I BbrPI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII BstBI BstXI ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FseI HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tsp509I XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 464 GGTACC G^GTACC
AflII 3142 CTTAAG C^TTAAG
AgeI 3036 ACCGGT A^CCGGT
AhdI 3329 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 714 GTGCAC G^TGCAC
ApaLI 714 GTGCAC G^TGCAC
Asp718I 464 GGTACC G^GTACC
AspEI 3329 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1814 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3373 CCTAGG C^CTAGG
BcgI 66 GCACCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1167 TGATCA T^GATCA
BfrI 3142 CTTAAG C^TTAAG
BlnI 3373 CCTAGG C^CTAGG
BsaAI 589 CACGTA [CT]AC^GT[GA]
BsaWI 3036 ACCGGT [AT]^CCGG[AT]
BsmBI 2824 CGTCTC CGTCTC(1/5)
BssSI 2444 CTCGTG CACGAG(-5/-1)
Bst1107I 3242 GTATAC GTA^TAC
BstZ17I 3242 GTATAC GTA^TAC
EagI 2391 CGGCCG C^GGCCG
Eam1105I 3329 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2391 CGGCCG C^GGCCG
Eco52I 2391 CGGCCG C^GGCCG
FbaI 1167 TGATCA T^GATCA
KpnI 464 GGTACC GGTAC^C
NaeI 3453 GCCGGC GCC^GGC
NcoI 468 CCATGG C^CATGG
NgoMIV 3453 GCCGGC G^CCGGC
PaeR7I 329 CTCGAG C^TCGAG
PflFI 1814 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3036 ACCGGT A^CCGGT
PshAI 1754 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SphI 669 GCATGC GCATG^C
StuI 2466 AGGCCT AGG^CCT
Tth111I 1814 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4480 TCTAGA T^CTAGA
XhoI 329 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 2325 ACGTGT A^C[GA][CT]GT
3691 ACGTGT
AocI 2917 CCTGAGG CC^T[ACGT]AGG
4135 CCTGAGG
Aor51HI 729 AGCGCT AGC^GCT
2028 AGCGCT
BamHI 376 GGATCC G^GATCC
1743 GGATCC
BbeI 3671 GGCGCC GGCGC^C
3716 GGCGCC
BbsI 3220 GAAGAC GAAGAC(2/6)
1889 GTCTTC
BciVI 1183 GGATAC GTATCC(6/5)
908 GTATCC
BfaI 3374 CTAG C^TAG
4481 CTAG
BglII 1665 AGATCT A^GATCT
3473 AGATCT
BpuAI 3220 GAAGAC GAAGAC(2/6)
1889 GTCTTC
BsiEI 1093 CGACCG CG[GA][CT]^CG
2391 CGGCCG
Bsp1407I 441 TGTACA T^GTACA
2632 TGTACA
BspHI 895 TCATGA T^CATGA
2691 TCATGA
BsrDI 1699 CATTGC GCAATG(2/0)
3960 GCAATG
BsrGI 441 TGTACA T^GTACA
2632 TGTACA
Bsu36I 2917 CCTGAGG CC^T[ACGT]AGG
4135 CCTGAGG
Eco47III 729 AGCGCT AGC^GCT
2028 AGCGCT
Eco81I 2917 CCTGAGG CC^T[ACGT]AGG
4135 CCTGAGG
EcoNI 2196 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4291 CCTTCCCCAGG
HincII 118 GTCGAC GT[CT]^[GA]AC
1091 GTCGAC
HindII 118 GTCGAC GT[CT]^[GA]AC
1091 GTCGAC
KasI 3671 GGCGCC G^GCGCC
3716 GGCGCC
KspI 3111 CCGCGG CCGC^GG
3712 CCGCGG
MaeI 3374 CTAG C^TAG
4481 CTAG
MseI 135 TTAA T^TAA
3143 TTAA
NarI 3671 GGCGCC GG^CGCC
3716 GGCGCC
RcaI 895 TCATGA T^CATGA
2691 TCATGA
SacII 3111 CCGCGG CCGC^GG
3712 CCGCGG
SalI 118 GTCGAC G^TCGAC
1091 GTCGAC
SapI 802 GAAGAGC GCTCTTC(1/4)
1645 GCTCTTC
SfoI 3671 GGCGCC GGC^GCC
3716 GGCGCC
SmaI 18 CCCGGG CCC^GGG
251 CCCGGG
SspBI 441 TGTACA T^GTACA
2632 TGTACA
Tru9I 135 TTAA T^TAA
3143 TTAA
XmaCI 18 CCCGGG C^CCGGG
251 CCCGGG
XmaI 18 CCCGGG C^CCGGG
251 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI ApaI
AspHI AvaI AvaII AviII BalI
BanI BanII BbvCI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII