##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
Asp700I AspEI AviII BamHI BbrPI
BcgI BciVI BclI BfrI BsaAI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI BstBI BstEII BstPI Bsu36I
ClaI CpoI DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1681 GGTACC G^GTACC
AcyI 782 GGCGCC G[GA]^CG[CT]C
AseI 3263 ATTAAT AT^TAAT
AsnI 3263 ATTAAT AT^TAAT
Asp718I 1681 GGTACC G^GTACC
AspHI 4130 GTGCTC G[AT]GC[AT]^C
BalI 911 TGGCCA TGG^CCA
BbeI 782 GGCGCC GGCGC^C
BsaHI 782 GGCGCC G[GA]^CG[CT]C
BsiHKAI 4130 GTGCTC G[AT]GC[AT]^C
BspLU11I 1021 ACATGT A^CATGT
BsrBI 765 GAGCGG CCGCTC(-3/-3)
BsrFI 1114 ACCGGC [GA]^CCGG[CT]
Cfr10I 1114 ACCGGC [GA]^CCGG[CT]
DraI 3976 TTTAAA TTT^AAA
DraIII 2694 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 782 GGCGCC G[GA]^CG[CT]C
KasI 782 GGCGCC G^GCGCC
KpnI 1681 GGTACC GGTAC^C
KspI 31 CCGCGG CCGC^GG
MluNI 911 TGGCCA TGG^CCA
MscI 911 TGGCCA TGG^CCA
NarI 782 GGCGCC GG^CGCC
NdeI 3325 CATATG CA^TATG
PshAI 41 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3263 ATTAAT AT^TAAT
PstI 2655 CTGCAG CTGCA^G
SacII 31 CCGCGG CCGC^GG
ScaI 1963 AGTACT AGT^ACT
SfoI 782 GGCGCC GGC^GCC
TaqI 2119 TCGA T^CGA
TthHB8I 2119 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 32 CGCG CG^CG
1905 CGCG
Aor51HI 1008 AGCGCT AGC^GCT
1687 AGCGCT
ApaI 2956 GGGCCC GGGCC^C
3748 GGGCCC
AspI 82 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
537 GACAGAGTC
BbvCI 2590 CCTCAGC CCTCAGC(-5/-2)
3174 CCTCAGC
BglII 709 AGATCT A^GATCT
3515 AGATCT
BlpI 140 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
Bpu1102I 140 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
BsaBI 1652 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2534 GATCAGCATC
BsiEI 34 CGGCCG CG[GA][CT]^CG
917 CGGCCG
BsrDI 485 CATTGC GCAATG(2/0)
2420 CATTGC
Bst1107I 2223 GTATAC GTA^TAC
3316 GTATAC
BstAPI 1130 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2648 GCAGGTGCTGC
BstUI 32 CGCG CG^CG
1905 CGCG
BstZ17I 2223 GTATAC GTA^TAC
3316 GTATAC
CelII 140 GCTTAGC GC^T[ACGT]AGC
2923 GCTGAGC
EagI 34 CGGCCG C^GGCCG
917 CGGCCG
EclXI 34 CGGCCG C^GGCCG
917 CGGCCG
Eco47III 1008 AGCGCT AGC^GCT
1687 AGCGCT
Eco52I 34 CGGCCG C^GGCCG
917 CGGCCG
HgaI 1162 GCGTC GACGC(5/10)
4047 GCGTC
HincII 1155 GTCAAC GT[CT]^[GA]AC
2447 GTTGAC
HindII 1155 GTCAAC GT[CT]^[GA]AC
2447 GTTGAC
MamI 1652 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2534 GATCAGCATC
MvnI 32 CGCG CG^CG
1905 CGCG
NcoI 3083 CCATGG C^CATGG
3343 CCATGG
PflFI 82 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
537 GACAGAGTC
PflMI 1859 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4200 CCACATGCTGG
SphI 506 GCATGC GCATG^C
1048 GCATGC
SspI 553 AATATT AAT^ATT
4314 AATATT
Tth111I 82 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
537 GACAGAGTC
Van91I 1859 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4200 CCACATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaI
AvaII AvrII BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmaCI XmaI