##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AviII BbrPI
BciVI BfrI BglI BglII BsiEI
BsiWI BspDI BspLU11I BsrFI BssHII
Bst1107I BstUI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FseI
FspI HgaI KspI MluI MvnI
NaeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SgrAI SphI Sse8387I
XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
Acc65I 3571 GGTACC G^GTACC
AccI 2722 GTAGAC GT^[AC][GT]AC
AccIII 604 TCCGGA T^CCGGA
AcyI 2948 GGCGCC G[GA]^CG[CT]C
AhdI 2575 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3527 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3571 GGTACC G^GTACC
AspEI 2575 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3224 GTGCTC G[AT]GC[AT]^C
AspI 2826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2948 GGCGCC GGCGC^C
BcgI 2908 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3877 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3877 GCTTAGC GC^T[ACGT]AGC
BsaAI 2765 TACGTA [CT]AC^GT[GA]
BsaBI 165 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2948 GGCGCC G[GA]^CG[CT]C
BsaWI 604 TCCGGA [AT]^CCGG[AT]
BseAI 604 TCCGGA T^CCGGA
BsgI 2857 GTGCAG GTGCAG(16/14)
BsiHKAI 3224 GTGCTC G[AT]GC[AT]^C
BsmBI 7 GAGACG CGTCTC(1/5)
Bsp1407I 3462 TGTACA T^GTACA
BspEI 604 TCCGGA T^CCGGA
BspMI 524 ACCTGC ACCTGC(4/8)
BsrDI 1810 GCAATG GCAATG(2/0)
BsrGI 3462 TGTACA T^GTACA
BssSI 582 CACGAG CACGAG(-5/-1)
BstBI 1471 TTCGAA TT^CGAA
BstEII 1214 GGTGACC G^GT[ACGT]ACC
BstPI 1214 GGTGACC G^GT[ACGT]ACC
CelII 3877 GCTTAGC GC^T[ACGT]AGC
CfoI 2949 GCGC GCG^C
DraIII 2283 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2575 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1214 GGTGACC G^GT[ACGT]ACC
HaeII 2948 GGCGCC [GA]GCGC^[CT]
HhaI 2949 GCGC GCG^C
Hin1I 2948 GGCGCC G[GA]^CG[CT]C
HinP1I 2949 GCGC G^CGC
HpaI 1059 GTTAAC GTT^AAC
KasI 2948 GGCGCC G^GCGCC
KpnI 3571 GGTACC GGTAC^C
MaeII 2766 ACGT A^CGT
MamI 165 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 400 CAATTG C^AATTG
MroI 604 TCCGGA T^CCGGA
MunI 400 CAATTG C^AATTG
NarI 2948 GGCGCC GG^CGCC
NdeI 718 CATATG CA^TATG
NheI 3937 GCTAGC G^CTAGC
NspV 1471 TTCGAA TT^CGAA
PflFI 2826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4105 GGGTCCT [GA]G^G[AT]CC[CT]
SexAI 2635 ACCTGGT A^CC[AT]GGT
SfoI 2948 GGCGCC GGC^GCC
SfuI 1471 TTCGAA TT^CGAA
SmaI 2896 CCCGGG CCC^GGG
SmlI 1177 CTCAAG C^T[CT][GA]AG
SnaBI 2765 TACGTA TAC^GTA
SspBI 3462 TGTACA T^GTACA
StuI 3885 AGGCCT AGG^CCT
SwaI 3439 ATTTAAAT ATTT^AAAT
Tth111I 2826 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1863 TCTAGA T^CTAGA
XmaCI 2896 CCCGGG C^CCGGG
XmaI 2896 CCCGGG C^CCGGG
XmnI 3527 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1312 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1315 CAGCTGCTG
AocI 1602 CCTGAGG CC^T[ACGT]AGG
1668 CCTGAGG
BalI 200 TGGCCA TGG^CCA
3011 TGGCCA
BamHI 947 GGATCC G^GATCC
3616 GGATCC
BbsI 626 GAAGAC GAAGAC(2/6)
3728 GAAGAC
BbvCI 2871 CCTCAGC CCTCAGC(-5/-2)
4048 GCTGAGG
BpuAI 626 GAAGAC GAAGAC(2/6)
3728 GAAGAC
BspHI 2479 TCATGA T^CATGA
3039 TCATGA
BsrBI 17 GAGCGG CCGCTC(-3/-3)
1581 GAGCGG
Bsu36I 1602 CCTGAGG CC^T[ACGT]AGG
1668 CCTGAGG
EaeI 200 TGGCCA [CT]^GGCC[GA]
3011 TGGCCA
Eco81I 1602 CCTGAGG CC^T[ACGT]AGG
1668 CCTGAGG
EcoT22I 2785 ATGCAT ATGCA^T
3715 ATGCAT
HindIII 122 AAGCTT A^AGCTT
4169 AAGCTT
MluNI 200 TGGCCA TGG^CCA
3011 TGGCCA
MscI 200 TGGCCA TGG^CCA
3011 TGGCCA
MspA1I 486 CAGCTG C[AC]G^C[GT]G
1315 CAGCTG
NsiI 2785 ATGCAT ATGCA^T
3715 ATGCAT
NspI 280 ACATGC [GA]CATG^[CT]
3944 GCATGT
PvuII 486 CAGCTG CAG^CTG
1315 CAGCTG
RcaI 2479 TCATGA T^CATGA
3039 TCATGA
SpeI 570 ACTAGT A^CTAGT
3803 ACTAGT
XcmI 1091 CCAGGCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2965 CCAGCTAATTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII AvrII BanI
BanII BbvI BclI BcnI BfaI
BlnI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII