##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AlwNI AocI Aor51HI
ApaI AscI Asp718I AvrII BanII
BbeI BbrPI BclI BfrI BglI
BlnI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FbaI FseI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 2814 ACATGT A^C[GA][CT]GT
AhdI 3752 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3112 GTGCAC G^TGCAC
ApaLI 3112 GTGCAC G^TGCAC
AspEI 3752 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4066 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3428 CCCGAG C^[CT]CG[GA]G
AviII 3104 TGCGCA TGC^GCA
BamHI 1461 GGATCC G^GATCC
BbsI 2935 GTCTTC GAAGAC(2/6)
BcgI 432 GCAGAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 241 GGATAC GTATCC(6/5)
BlpI 3258 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3258 GCTGAGC GC^T[ACGT]AGC
BpuAI 2935 GTCTTC GAAGAC(2/6)
BsaWI 470 ACCGGA [AT]^CCGG[AT]
BsmBI 474 GAGACG CGTCTC(1/5)
BsoBI 3428 CCCGAG C^[CT]CG[GA]G
BspLU11I 2814 ACATGT A^CATGT
BsrDI 743 CATTGC GCAATG(2/0)
BsrFI 428 GCCGGC [GA]^CCGG[CT]
Bst1107I 3361 GTATAC GTA^TAC
BstZ17I 3361 GTATAC GTA^TAC
CelII 3258 GCTGAGC GC^T[ACGT]AGC
Cfr10I 428 GCCGGC [GA]^CCGG[CT]
DraI 3942 TTTAAA TTT^AAA
Eam1105I 3752 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3020 GAATTC G^AATTC
FspI 3104 TGCGCA TGC^GCA
HgaI 1775 GACGC GACGC(5/10)
HincII 3135 GTTGAC GT[CT]^[GA]AC
HindII 3135 GTTGAC GT[CT]^[GA]AC
HindIII 2518 AAGCTT A^AGCTT
MfeI 1787 CAATTG C^AATTG
MunI 1787 CAATTG C^AATTG
NaeI 428 GCCGGC GCC^GGC
NgoMIV 428 GCCGGC G^CCGGC
PflFI 4066 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2588 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2163 CTGCAG CTGCA^G
SexAI 1430 ACCAGGT A^CC[AT]GGT
SphI 804 GCATGC GCATG^C
Tth111I 4066 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2588 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2274 CCAGGAGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1114 GTAGAC GT^[AC][GT]AC
3361 GTATAC
AccII 30 CGCG CG^CG
2560 CGCG
AseI 778 ATTAAT AT^TAAT
2572 ATTAAT
AsnI 778 ATTAAT AT^TAAT
2572 ATTAAT
Asp700I 898 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3890 GAAGTGTTTC
AspHI 1441 GTGCTC G[AT]GC[AT]^C
3112 GTGCAC
BanI 568 GGCACC G^G[CT][GA]CC
3725 GGCACC
BbvCI 3775 CCTCAGC CCTCAGC(-5/-2)
973 GCTGAGG
BcnI 228 CCGGG CC^[GC]GG
3963 CCGGG
BglII 172 AGATCT A^GATCT
623 AGATCT
BmyI 1441 GTGCTC G[AGT]GC[ACT]^C
3112 GTGCAC
BsiHKAI 1441 GTGCTC G[AT]GC[AT]^C
3112 GTGCAC
BsmI 2269 GAATGC GAATGC(1/-1)
2842 GAATGC
Bsp1286I 1441 GTGCTC G[AGT]GC[ACT]^C
3112 GTGCAC
BspMI 3086 ACCTGC ACCTGC(4/8)
2165 GCAGGT
BstAPI 1993 GCAACTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3707 GCAAATGGTGC
BstUI 30 CGCG CG^CG
2560 CGCG
CfoI 1596 GCGC GCG^C
3105 GCGC
DraII 1643 AGGCCCC [GA]G^G[ACGT]CC[CT]
3370 GGGGCCT
DsaI 1334 CCATGG C^C[GA][CT]GG
2438 CCATGG
EcoNI 1208 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1425 CCTAGACCAGG
EcoO109I 1643 AGGCCCC [GA]G^G[ACGT]CC[CT]
3370 GGGGCCT
EcoT22I 802 ATGCAT ATGCA^T
2281 ATGCAT
HhaI 1596 GCGC GCG^C
3105 GCGC
HinP1I 1596 GCGC G^CGC
3105 GCGC
MaeII 1370 ACGT A^CGT
2398 ACGT
MvnI 30 CGCG CG^CG
2560 CGCG
NciI 228 CCGGG CC^[GC]GG
3963 CCGGG
NcoI 1334 CCATGG C^CATGG
2438 CCATGG
NsiI 802 ATGCAT ATGCA^T
2281 ATGCAT
PleI 154 GAGTC GAGTC(4/5)
2504 GAGTC
PshBI 778 ATTAAT AT^TAAT
2572 ATTAAT
XmnI 898 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3890 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BbvI BfaI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII