##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Alw44I AocI Aor51HI ApaLI AscI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BglI
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FseI
FspI KasI KspI MluI MroI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2610 GGTACC G^GTACC
AcyI 808 GACGCC G[GA]^CG[CT]C
AflII 3918 CTTAAG C^TTAAG
AhdI 90 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2833 GGGCCC GGGCC^C
AseI 2182 ATTAAT AT^TAAT
AsnI 2182 ATTAAT AT^TAAT
Asp700I 686 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2610 GGTACC G^GTACC
AspEI 90 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1676 TGGCCA TGG^CCA
BclI 2780 TGATCA T^GATCA
BfrI 3918 CTTAAG C^TTAAG
BglII 4118 AGATCT A^GATCT
BsaHI 808 GACGCC G[GA]^CG[CT]C
Bsp1407I 2115 TGTACA T^GTACA
BsrFI 3710 GCCGGC [GA]^CCGG[CT]
BsrGI 2115 TGTACA T^GTACA
Cfr10I 3710 GCCGGC [GA]^CCGG[CT]
DraIII 2702 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2358 GACAATTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1676 TGGCCA [CT]^GGCC[GA]
Eam1105I 90 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 418 GAATTC G^AATTC
EcoRV 319 GATATC GAT^ATC
EcoT22I 2338 ATGCAT ATGCA^T
FbaI 2780 TGATCA T^GATCA
HaeII 134 AGCGCC [GA]GCGC^[CT]
HgaI 808 GACGC GACGC(5/10)
Hin1I 808 GACGCC G[GA]^CG[CT]C
HindIII 1198 AAGCTT A^AGCTT
HpaI 2670 GTTAAC GTT^AAC
KpnI 2610 GGTACC GGTAC^C
MfeI 2360 CAATTG C^AATTG
MluNI 1676 TGGCCA TGG^CCA
MscI 1676 TGGCCA TGG^CCA
MspA1I 1058 CAGCTG C[AC]G^C[GT]G
MunI 2360 CAATTG C^AATTG
NaeI 3710 GCCGGC GCC^GGC
NgoMIV 3710 GCCGGC G^CCGGC
NsiI 2338 ATGCAT ATGCA^T
PaeR7I 3461 CTCGAG C^TCGAG
PshBI 2182 ATTAAT AT^TAAT
PvuII 1058 CAGCTG CAG^CTG
SacI 1911 GAGCTC GAGCT^C
SapI 2710 GAAGAGC GCTCTTC(1/4)
ScaI 4155 AGTACT AGT^ACT
SmaI 2748 CCCGGG CCC^GGG
SspBI 2115 TGTACA T^GTACA
StuI 2856 AGGCCT AGG^CCT
XhoI 3461 CTCGAG C^TCGAG
XmaCI 2748 CCCGGG C^CCGGG
XmaI 2748 CCCGGG C^CCGGG
XmnI 686 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 10 CGCG CG^CG
862 CGCG
AflIII 2112 ACATGT A^C[GA][CT]GT
4005 ACATGT
AlwNI 1916 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3829 CAGGAACTG
AspHI 1911 GAGCTC G[AT]GC[AT]^C
2720 GAGCAC
AvaI 2748 CCCGGG C^[CT]CG[GA]G
3461 CTCGAG
AvaII 775 GGTCC G^G[AT]CC
1153 GGACC
BanI 2610 GGTACC G^G[CT][GA]CC
2758 GGCACC
BlpI 62 GCTGAGC GC^T[ACGT]AGC
1195 GCTAAGC
Bpu1102I 62 GCTGAGC GC^T[ACGT]AGC
1195 GCTAAGC
BsaBI 1936 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3429 GATAAAAATC
BsaI 70 GAGACC GGTCTC(1/5)
106 GAGACC
BsgI 2621 CTGCAC GTGCAG(16/14)
3323 GTGCAG
BsiHKAI 1911 GAGCTC G[AT]GC[AT]^C
2720 GAGCAC
BsmI 2240 GAATGC GAATGC(1/-1)
2559 GAATGC
BsoBI 2748 CCCGGG C^[CT]CG[GA]G
3461 CTCGAG
BspLU11I 2112 ACATGT A^CATGT
4005 ACATGT
BspMI 2131 GCAGGT ACCTGC(4/8)
3496 GCAGGT
BsrBI 35 GAGCGG CCGCTC(-3/-3)
65 GAGCGG
BsrDI 2313 CATTGC GCAATG(2/0)
148 GCAATG
BssSI 47 CACGAG CACGAG(-5/-1)
130 CACGAG
BstUI 10 CGCG CG^CG
862 CGCG
CelII 62 GCTGAGC GC^T[ACGT]AGC
1195 GCTAAGC
DraI 2475 TTTAAA TTT^AAA
4079 TTTAAA
EcoT14I 188 CCAAGG C^C[AT][AT]GG
212 CCTTGG
FauI 2591 CCCGC CCCGC(4/6)
11 GCGGG
HincII 2670 GTTAAC GT[CT]^[GA]AC
3547 GTTGAC
HindII 2670 GTTAAC GT[CT]^[GA]AC
3547 GTTGAC
MaeII 1242 ACGT A^CGT
3897 ACGT
MamI 1936 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3429 GATAAAAATC
MvnI 10 CGCG CG^CG
862 CGCG
PpuMI 774 AGGTCCT [GA]G^G[AT]CC[CT]
1152 AGGACCT
PshAI 72 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3991 GACAGTGGTC
StyI 188 CCAAGG C^C[AT][AT]GG
212 CCTTGG
XbaI 736 TCTAGA T^CTAGA
2091 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII