##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BspDI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MluI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 199 CGCG CG^CG
AccIII 2744 TCCGGA T^CCGGA
AclI 1915 AACGTT AA^CGTT
AflIII 838 ACATGT A^C[GA][CT]GT
AlwNI 2474 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3378 GGGCCC GGGCC^C
AseI 3405 ATTAAT AT^TAAT
AsnI 3405 ATTAAT AT^TAAT
Asp700I 3172 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3304 CCCGAG C^[CT]CG[GA]G
BamHI 466 GGATCC G^GATCC
BglII 3372 AGATCT A^GATCT
BsaI 1967 GAGACC GGTCTC(1/5)
BseAI 2744 TCCGGA T^CCGGA
BsoBI 3304 CCCGAG C^[CT]CG[GA]G
BspEI 2744 TCCGGA T^CCGGA
BspHI 3711 TCATGA T^CATGA
BspLU11I 838 ACATGT A^CATGT
BsrDI 4465 GCAATG GCAATG(2/0)
BstEII 1898 GGTTACC G^GT[ACGT]ACC
BstPI 1898 GGTTACC G^GT[ACGT]ACC
BstUI 199 CGCG CG^CG
BstXI 2891 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 198 GCGC GCG^C
DsaI 89 CCATGG C^C[GA][CT]GG
EcoNI 1326 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1898 GGTTACC G^GT[ACGT]ACC
EcoRV 3932 GATATC GAT^ATC
EcoT22I 4250 ATGCAT ATGCA^T
HhaI 198 GCGC GCG^C
HinP1I 198 GCGC G^CGC
HindIII 602 AAGCTT A^AGCTT
HpaI 4561 GTTAAC GTT^AAC
MfeI 1886 CAATTG C^AATTG
MroI 2744 TCCGGA T^CCGGA
MunI 1886 CAATTG C^AATTG
MvnI 199 CGCG CG^CG
NaeI 2509 GCCGGC GCC^GGC
NcoI 89 CCATGG C^CATGG
NgoMIV 2509 GCCGGC G^CCGGC
NsiI 4250 ATGCAT ATGCA^T
PpuMI 1025 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3405 ATTAAT AT^TAAT
Psp1406I 1915 AACGTT AA^CGTT
RcaI 3711 TCATGA T^CATGA
SbfI 73 CCTGCAGG CCTGCA^GG
ScaI 1042 AGTACT AGT^ACT
SgrAI 31 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 73 CCTGCAGG CCTGCA^GG
SspI 4639 AATATT AAT^ATT
SwaI 4591 ATTTAAAT ATTT^AAAT
XmnI 3172 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 328 GTCTAC GT^[AC][GT]AC
3100 GTAGAC
AflII 3223 CTTAAG C^TTAAG
3529 CTTAAG
AocI 1330 CCTAAGG CC^T[ACGT]AGG
4448 CCTTAGG
BclI 1281 TGATCA T^GATCA
3714 TGATCA
BcnI 224 CCGGG CC^[GC]GG
3819 CCCGG
BfrI 3223 CTTAAG C^TTAAG
3529 CTTAAG
BsaWI 2153 ACCGGA [AT]^CCGG[AT]
2744 TCCGGA
BsmI 1605 GAATGC GAATGC(1/-1)
2894 GCATTC
Bsu36I 1330 CCTAAGG CC^T[ACGT]AGG
4448 CCTTAGG
DraII 1025 AGGACCC [GA]G^G[ACGT]CC[CT]
3378 GGGCCCC
DraIII 2107 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4274 CACCTAGTG
Eco81I 1330 CCTAAGG CC^T[ACGT]AGG
4448 CCTTAGG
EcoO109I 1025 AGGACCC [GA]G^G[ACGT]CC[CT]
3378 GGGCCCC
EcoT14I 89 CCATGG C^C[AT][AT]GG
1074 CCTTGG
FbaI 1281 TGATCA T^GATCA
3714 TGATCA
HgaI 4599 GACGC GACGC(5/10)
200 GCGTC
NciI 224 CCGGG CC^[GC]GG
3819 CCCGG
SacI 65 GAGCTC GAGCT^C
3827 GAGCTC
StyI 89 CCATGG C^C[AT][AT]GG
1074 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRI EcoRII FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII