##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AvaI AviII BalI BamHI
BbeI BbvCI BglI BglII BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstUI BstXI
BstZ17I ClaI CpoI DraII DraIII
EaeI EagI EclXI Eco47III Eco52I
EcoO109I FseI FspI Hin1I HincII
HindII HpaI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PleI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1456 GGTACC G^GTACC
AflII 2797 CTTAAG C^TTAAG
AhdI 3713 GACTTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4077 ATTAAT AT^TAAT
AsnI 4077 ATTAAT AT^TAAT
Asp718I 1456 GGTACC G^GTACC
AspEI 3713 GACTTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2452 CCTAGG C^CTAGG
BbrPI 3472 CACGTG CAC^GTG
BcgI 1737 CGACAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3404 GTATCC GTATCC(6/5)
BcnI 1266 CCCGG CC^[GC]GG
BfrI 2797 CTTAAG C^TTAAG
BlnI 2452 CCTAGG C^CTAGG
BsaAI 3472 CACGTG [CT]AC^GT[GA]
BsmBI 1210 GAGACG CGTCTC(1/5)
Bsp1407I 2340 TGTACA T^GTACA
BspHI 1083 TCATGA T^CATGA
BspLU11I 3871 ACATGT A^CATGT
BsrDI 1934 CATTGC GCAATG(2/0)
BsrFI 37 ACCGGC [GA]^CCGG[CT]
BsrGI 2340 TGTACA T^GTACA
BstEII 2434 GGTCACC G^GT[ACGT]ACC
BstPI 2434 GGTCACC G^GT[ACGT]ACC
CfoI 1708 GCGC GCG^C
Cfr10I 37 ACCGGC [GA]^CCGG[CT]
DrdI 262 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 254 CCGTGG C^C[GA][CT]GG
Eam1105I 3713 GACTTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2434 GGTCACC G^GT[ACGT]ACC
EcoRI 1182 GAATTC G^AATTC
EcoT22I 1931 ATGCAT ATGCA^T
FauI 1153 CCCGC CCCGC(4/6)
HaeII 1707 AGCGCC [GA]GCGC^[CT]
HhaI 1708 GCGC GCG^C
HinP1I 1708 GCGC G^CGC
KpnI 1456 GGTACC GGTAC^C
MslI 1282 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1531 CAGCTG C[AC]G^C[GT]G
NciI 1266 CCCGG CC^[GC]GG
NsiI 1931 ATGCAT ATGCA^T
PmaCI 3472 CACGTG CAC^GTG
PmlI 3472 CACGTG CAC^GTG
PshBI 4077 ATTAAT AT^TAAT
PstI 1336 CTGCAG CTGCA^G
PvuII 1531 CAGCTG CAG^CTG
RcaI 1083 TCATGA T^CATGA
SapI 1669 GAAGAGC GCTCTTC(1/4)
SphI 3747 GCATGC GCATG^C
SspBI 2340 TGTACA T^GTACA
XbaI 2316 TCTAGA T^CTAGA
XcmI 1133 CCAACATTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2691 GTCTAC GT^[AC][GT]AC
3443 GTAGAC
AflIII 3473 ACGTGT A^C[GA][CT]GT
3871 ACATGT
AocI 225 CCTAAGG CC^T[ACGT]AGG
237 CCTCAGG
AspHI 764 GAGCAC G[AT]GC[AT]^C
1518 GAGCAC
AspI 2598 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2911 GACTATGTC
BanI 34 GGCACC G^G[CT][GA]CC
1456 GGTACC
BanII 205 GAGCCC G[GA]GC[CT]^C
811 GAGCCC
BbsI 1699 GAAGAC GAAGAC(2/6)
2959 GAAGAC
BclI 161 TGATCA T^GATCA
2274 TGATCA
BpuAI 1699 GAAGAC GAAGAC(2/6)
2959 GAAGAC
BseRI 524 CTCCTC GAGGAG(10/8)
858 GAGGAG
BsgI 1502 CTGCAC GTGCAG(16/14)
2656 GTGCAG
BsiHKAI 764 GAGCAC G[AT]GC[AT]^C
1518 GAGCAC
BspMI 1100 ACCTGC ACCTGC(4/8)
1938 GCAGGT
BstAPI 144 GCAAAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
766 GCACAAGATGC
BstYI 1592 AGATCC [GA]^GATC[CT]
1826 AGATCC
Bsu36I 225 CCTAAGG CC^T[ACGT]AGG
237 CCTCAGG
Eco81I 225 CCTAAGG CC^T[ACGT]AGG
237 CCTCAGG
EcoRV 2076 GATATC GAT^ATC
2734 GATATC
FbaI 161 TGATCA T^GATCA
2274 TGATCA
HaeIII 199 GGCC GG^CC
992 GGCC
HapII 38 CCGG C^CGG
1267 CCGG
HgaI 1212 GACGC GACGC(5/10)
1218 GACGC
HpaII 38 CCGG C^CGG
1267 CCGG
MflI 1592 AGATCC [GA]^GATC[CT]
1826 AGATCC
MspI 38 CCGG C^CGG
1267 CCGG
NspI 3747 GCATGC [GA]CATG^[CT]
3871 ACATGT
PflFI 2598 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2911 GACTATGTC
SmlI 1654 CTTGAG C^T[CT][GA]AG
2797 CTTAAG
Tth111I 2598 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2911 GACTATGTC
XhoII 1592 AGATCC [GA]^GATC[CT]
1826 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstBI
BstF5I BstNI Cac8I CelII Cfr13I
DdeI DpnII DraI EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MnlI MseI MunI MvaI MwoI
NdeII NlaIII NlaIV NspV PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmnI