##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I BbrPI BciVI BfrI BsaAI
BsaI BsiEI BsiWI BsmI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoT22I FseI
HpaI KspI MfeI MunI NaeI
NcoI NgoMIV NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI Sse8387I
SspBI StuI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 968 GGTACC G^GTACC
AccIII 1965 TCCGGA T^CCGGA
AhdI 2998 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3499 CCTCAGG CC^T[ACGT]AGG
Asp718I 968 GGTACC G^GTACC
AspEI 2998 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1803 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 447 TGCGCA TGC^GCA
BalI 3524 TGGCCA TGG^CCA
BbeI 147 GGCGCC GGCGC^C
BbsI 3250 GTCTTC GAAGAC(2/6)
BcgI 1843 GCATGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 753 TGATCA T^GATCA
BglI 3428 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1987 AGATCT A^GATCT
BlpI 1636 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1636 GCTCAGC GC^T[ACGT]AGC
BpuAI 3250 GTCTTC GAAGAC(2/6)
BsaBI 1988 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1965 TCCGGA T^CCGGA
BspEI 1965 TCCGGA T^CCGGA
BspHI 1680 TCATGA T^CATGA
BspLU11I 2782 ACATGT A^CATGT
BsrBI 2318 GAGCGG CCGCTC(-3/-3)
BsrFI 2889 GCCGGT [GA]^CCGG[CT]
BstAPI 490 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 867 TTCGAA TT^CGAA
Bsu36I 3499 CCTCAGG CC^T[ACGT]AGG
CelII 1636 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2889 GCCGGT [GA]^CCGG[CT]
DsaI 1722 CCACGG C^C[GA][CT]GG
Eam1105I 2998 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3499 CCTCAGG CC^T[ACGT]AGG
EcoRI 870 GAATTC G^AATTC
EcoRV 719 GATATC GAT^ATC
FbaI 753 TGATCA T^GATCA
FspI 447 TGCGCA TGC^GCA
HincII 2786 GTTGAC GT[CT]^[GA]AC
HindII 2786 GTTGAC GT[CT]^[GA]AC
KasI 147 GGCGCC G^GCGCC
KpnI 968 GGTACC GGTAC^C
MamI 1988 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3809 ACGCGT A^CGCGT
MluNI 3524 TGGCCA TGG^CCA
MroI 1965 TCCGGA T^CCGGA
MscI 3524 TGGCCA TGG^CCA
NarI 147 GGCGCC GG^CGCC
NdeI 948 CATATG CA^TATG
NheI 1151 GCTAGC G^CTAGC
NspV 867 TTCGAA TT^CGAA
PaeR7I 888 CTCGAG C^TCGAG
PflFI 1803 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1680 TCATGA T^CATGA
SapI 118 GCTCTTC GCTCTTC(1/4)
SfoI 147 GGCGCC GGC^GCC
SfuI 867 TTCGAA TT^CGAA
SpeI 1675 ACTAGT A^CTAGT
SphI 582 GCATGC GCATG^C
SspI 555 AATATT AAT^ATT
Tth111I 1803 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 789 CCAACCGATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 888 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1319 GTCTAC GT^[AC][GT]AC
1454 GTCTAC
AcyI 147 GGCGCC G[GA]^CG[CT]C
672 GACGCC
AflIII 2782 ACATGT A^C[GA][CT]GT
3809 ACGCGT
AvrII 3216 CCTAGG C^CTAGG
3929 CCTAGG
BbvCI 2359 CCTCAGC CCTCAGC(-5/-2)
2751 CCTCAGC
BlnI 3216 CCTAGG C^CTAGG
3929 CCTAGG
BsaHI 147 GGCGCC G[GA]^CG[CT]C
672 GACGCC
BsaWI 1491 TCCGGT [AT]^CCGG[AT]
1965 TCCGGA
BsrDI 444 CATTGC GCAATG(2/0)
766 CATTGC
BstEII 1351 GGTGACC G^GT[ACGT]ACC
3603 GGTCACC
BstPI 1351 GGTGACC G^GT[ACGT]ACC
3603 GGTCACC
DrdI 1928 GACCCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3241 GACTGCTCTGTC
EcoO65I 1351 GGTGACC G^GT[ACGT]ACC
3603 GGTCACC
HaeII 147 GGCGCC [GA]GCGC^[CT]
2772 AGCGCC
Hin1I 147 GGCGCC G[GA]^CG[CT]C
672 GACGCC
HindIII 350 AAGCTT A^AGCTT
1960 AAGCTT
PstI 982 CTGCAG CTGCA^G
2246 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII