##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BbeI BbrPI BcgI BclI
BcnI BglI BlnI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BssHII BstAPI BstEII
BstPI BstUI CfoI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1409 GGTACC G^GTACC
AflIII 1006 ACATGT A^C[GA][CT]GT
AlwNI 3548 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1409 GGTACC G^GTACC
BamHI 122 GGATCC G^GATCC
BanII 3847 GAGCCC G[GA]GC[CT]^C
BlpI 3855 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3855 GCTAAGC GC^T[ACGT]AGC
BsaAI 2968 TACGTA [CT]AC^GT[GA]
BsaBI 3257 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1006 ACATGT A^CATGT
BsrBI 550 GAGCGG CCGCTC(-3/-3)
BsrDI 2166 CATTGC GCAATG(2/0)
BsrFI 1141 ACCGGC [GA]^CCGG[CT]
BssSI 1808 CTCGTG CACGAG(-5/-1)
Bst1107I 3886 GTATAC GTA^TAC
BstBI 1397 TTCGAA TT^CGAA
BstZ17I 3886 GTATAC GTA^TAC
CelII 3855 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1141 ACCGGC [GA]^CCGG[CT]
DraIII 4013 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 515 CCATGG C^C[GA][CT]GG
EcoNI 1883 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 792 CCCGC CCCGC(4/6)
HapII 1142 CCGG C^CGG
HpaII 1142 CCGG C^CGG
KpnI 1409 GGTACC GGTAC^C
MaeII 2969 ACGT A^CGT
MamI 3257 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2602 CAGCTG C[AC]G^C[GT]G
MspI 1142 CCGG C^CGG
NcoI 515 CCATGG C^CATGG
NheI 1373 GCTAGC G^CTAGC
NspV 1397 TTCGAA TT^CGAA
PaeR7I 603 CTCGAG C^TCGAG
PflMI 1385 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3203 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 2602 CAGCTG CAG^CTG
SapI 842 GCTCTTC GCTCTTC(1/4)
SexAI 3614 ACCTGGT A^CC[AT]GGT
SfuI 1397 TTCGAA TT^CGAA
SnaBI 2968 TACGTA TAC^GTA
SpeI 684 ACTAGT A^CTAGT
SphI 2036 GCATGC GCATG^C
Van91I 1385 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1787 CCAGACTTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 603 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 2670 CTTAAG C^TTAAG
3312 CTTAAG
AocI 2049 CCTTAGG CC^T[ACGT]AGG
3563 CCTCAGG
AseI 2731 ATTAAT AT^TAAT
3727 ATTAAT
AsnI 2731 ATTAAT AT^TAAT
3727 ATTAAT
Asp700I 1226 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2352 GAAAATCTTC
BalI 758 TGGCCA TGG^CCA
898 TGGCCA
BanI 1409 GGTACC G^G[CT][GA]CC
1771 GGCACC
BbvCI 1290 GCTGAGG CCTCAGC(-5/-2)
3361 GCTGAGG
BciVI 453 GGATAC GTATCC(6/5)
787 GGATAC
BfrI 2670 CTTAAG C^TTAAG
3312 CTTAAG
BglII 3262 AGATCT A^GATCT
3672 AGATCT
BspHI 305 TCATGA T^CATGA
3072 TCATGA
Bsu36I 2049 CCTTAGG CC^T[ACGT]AGG
3563 CCTCAGG
Eco81I 2049 CCTTAGG CC^T[ACGT]AGG
3563 CCTCAGG
HgaI 309 GACGC GACGC(5/10)
1667 GCGTC
HincII 2593 GTTAAC GT[CT]^[GA]AC
3177 GTTAAC
HindII 2593 GTTAAC GT[CT]^[GA]AC
3177 GTTAAC
HpaI 2593 GTTAAC GTT^AAC
3177 GTTAAC
MluNI 758 TGGCCA TGG^CCA
898 TGGCCA
MscI 758 TGGCCA TGG^CCA
898 TGGCCA
PshAI 5 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
977 GACACCTGTC
PshBI 2731 ATTAAT AT^TAAT
3727 ATTAAT
RcaI 305 TCATGA T^CATGA
3072 TCATGA
TaqI 604 TCGA T^CGA
1398 TCGA
TthHB8I 604 TCGA T^CGA
1398 TCGA
XmnI 1226 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2352 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII