##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AvrII BbeI BciVI
BfrI BlnI BsaI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
HgaI HindIII HpaI KasI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I RsrII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 913 GACGTC GACGT^C
AcyI 913 GACGTC G[GA]^CG[CT]C
Alw44I 513 GTGCAC G^TGCAC
AocI 3190 CCTAAGG CC^T[ACGT]AGG
ApaI 43 GGGCCC GGGCC^C
ApaLI 513 GTGCAC G^TGCAC
BamHI 2030 GGATCC G^GATCC
BbsI 154 GAAGAC GAAGAC(2/6)
BcgI 2420 CGAATCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3180 TGATCA T^GATCA
BpuAI 154 GAAGAC GAAGAC(2/6)
BsaBI 990 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 913 GACGTC G[GA]^CG[CT]C
BsaWI 2272 ACCGGA [AT]^CCGG[AT]
BsgI 1146 CTGCAC GTGCAG(16/14)
BsiEI 48 CGATCG CG[GA][CT]^CG
Bsp1407I 3170 TGTACA T^GTACA
BspHI 2468 TCATGA T^CATGA
BsrBI 1417 GAGCGG CCGCTC(-3/-3)
BsrGI 3170 TGTACA T^GTACA
BssSI 1438 CTCGTG CACGAG(-5/-1)
BstEII 432 GGTGACC G^GT[ACGT]ACC
BstPI 432 GGTGACC G^GT[ACGT]ACC
Bsu36I 3190 CCTAAGG CC^T[ACGT]AGG
DrdI 1192 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3190 CCTAAGG CC^T[ACGT]AGG
EcoNI 759 CCTATTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 432 GGTGACC G^GT[ACGT]ACC
EcoRI 970 GAATTC G^AATTC
EcoRV 1810 GATATC GAT^ATC
FbaI 3180 TGATCA T^GATCA
HaeII 3310 AGCGCC [GA]GCGC^[CT]
Hin1I 913 GACGTC G[GA]^CG[CT]C
MamI 990 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3722 GCTAGC G^CTAGC
PaeR7I 1756 CTCGAG C^TCGAG
PvuI 48 CGATCG CGAT^CG
RcaI 2468 TCATGA T^CATGA
SexAI 1834 ACCTGGT A^CC[AT]GGT
SfiI 35 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3170 TGTACA T^GTACA
SspI 3865 AATATT AAT^ATT
XbaI 822 TCTAGA T^CTAGA
XhoI 1756 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 343 GGTACC G^GTACC
1673 GGTACC
Asp700I 2304 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2806 GAAAAGATTC
Asp718I 343 GGTACC G^GTACC
1673 GGTACC
AviII 560 TGCGCA TGC^GCA
3556 TGCGCA
BbvCI 2187 CCTCAGC CCTCAGC(-5/-2)
3106 CCTCAGC
BglI 36 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1470 GCCATTTTGGC
BglII 1116 AGATCT A^GATCT
1780 AGATCT
BlpI 2085 GCTCAGC GC^T[ACGT]AGC
3306 GCTGAGC
Bpu1102I 2085 GCTCAGC GC^T[ACGT]AGC
3306 GCTGAGC
BsrFI 1598 ACCGGC [GA]^CCGG[CT]
2505 GCCGGT
CelII 2085 GCTCAGC GC^T[ACGT]AGC
3306 GCTGAGC
Cfr10I 1598 ACCGGC [GA]^CCGG[CT]
2505 GCCGGT
DraIII 522 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1691 CACACTGTG
FspI 560 TGCGCA TGC^GCA
3556 TGCGCA
KpnI 343 GGTACC GGTAC^C
1673 GGTACC
KspI 38 CCGCGG CCGC^GG
60 CCGCGG
PflMI 1889 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3625 CCACTTCTTGG
PpuMI 546 AGGACCC [GA]G^G[AT]CC[CT]
1091 AGGACCT
SacII 38 CCGCGG CCGC^GG
60 CCGCGG
SmaI 87 CCCGGG CCC^GGG
1280 CCCGGG
Van91I 1889 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3625 CCACTTCTTGG
XmaCI 87 CCCGGG C^CCGGG
1280 CCCGGG
XmaI 87 CCCGGG C^CCGGG
1280 CCCGGG
XmnI 2304 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2806 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcnI BfaI BmyI
BpmI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII