##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BanI
BbeI BbrPI BbvCI BfrI BglI
BlnI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrFI BssHII
Bst1107I BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 3200 GTCTAC GT^[AC][GT]AC
AccII 1858 CGCG CG^CG
AclI 3086 AACGTT AA^CGTT
AcyI 3325 GACGCC G[GA]^CG[CT]C
AflIII 1458 ACGTGT A^C[GA][CT]GT
AhdI 2767 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3330 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3412 GGGCCC GGGCC^C
Asp700I 3757 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2767 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1394 TGGCCA TGG^CCA
BamHI 733 GGATCC G^GATCC
BcgI 1335 GCAGTTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 458 GGATAC GTATCC(6/5)
BclI 536 TGATCA T^GATCA
BcnI 3410 CCGGG CC^[GC]GG
BlpI 3312 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3312 GCTCAGC GC^T[ACGT]AGC
BsaAI 1457 TACGTG [CT]AC^GT[GA]
BsaHI 3325 GACGCC G[GA]^CG[CT]C
BsaWI 2733 ACCGGA [AT]^CCGG[AT]
BsgI 35 CTGCAC GTGCAG(16/14)
BspHI 2926 TCATGA T^CATGA
BssSI 2275 CTCGTG CACGAG(-5/-1)
BstAPI 3339 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3564 TTCGAA TT^CGAA
BstUI 1858 CGCG CG^CG
CelII 3312 GCTCAGC GC^T[ACGT]AGC
DraI 195 TTTAAA TTT^AAA
DraII 2718 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2668 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2542 GACATGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2767 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2718 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 536 TGATCA T^GATCA
Hin1I 3325 GACGCC G[GA]^CG[CT]C
MluNI 1394 TGGCCA TGG^CCA
MscI 1394 TGGCCA TGG^CCA
MvnI 1858 CGCG CG^CG
NciI 3410 CCGGG CC^[GC]GG
NspI 1877 ACATGC [GA]CATG^[CT]
NspV 3564 TTCGAA TT^CGAA
PpuMI 2718 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3086 AACGTT AA^CGTT
RcaI 2926 TCATGA T^CATGA
SacI 129 GAGCTC GAGCT^C
SapI 3757 GAAGAGC GCTCTTC(1/4)
ScaI 3030 AGTACT AGT^ACT
SfaNI 2936 GATGC GCATC(5/9)
SfuI 3564 TTCGAA TT^CGAA
SpeI 1323 ACTAGT A^CTAGT
SspI 436 AATATT AAT^ATT
XbaI 1796 TCTAGA T^CTAGA
XmnI 3757 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AseI 2042 ATTAAT AT^TAAT
2945 ATTAAT
AsnI 2042 ATTAAT AT^TAAT
2945 ATTAAT
BsmI 2245 GAATGC GAATGC(1/-1)
578 GCATTC
Bsp1407I 82 TGTACA T^GTACA
1783 TGTACA
BspMI 2975 ACCTGC ACCTGC(4/8)
3344 ACCTGC
BsrBI 2891 CCGCTC CCGCTC(-3/-3)
2877 GAGCGG
BsrGI 82 TGTACA T^GTACA
1783 TGTACA
BstEII 2034 GGTCACC G^GT[ACGT]ACC
2665 GGTCACC
BstPI 2034 GGTCACC G^GT[ACGT]ACC
2665 GGTCACC
CfoI 266 GCGC GCG^C
1193 GCGC
EcoNI 1475 CCTTAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2980 CCTTATCGAGG
EcoO65I 2034 GGTCACC G^GT[ACGT]ACC
2665 GGTCACC
EcoRI 2 GAATTC G^AATTC
2298 GAATTC
FauI 2722 CCCGC CCCGC(4/6)
2890 CCCGC
HapII 2734 CCGG C^CGG
3410 CCGG
HhaI 266 GCGC GCG^C
1193 GCGC
HinP1I 266 GCGC G^CGC
1193 GCGC
HpaII 2734 CCGG C^CGG
3410 CCGG
MslI 294 CATTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
774 CATAGTGATG
MspA1I 3390 CAGCTG C[AC]G^C[GT]G
3816 CAGCTG
MspI 2734 CCGG C^CGG
3410 CCGG
NcoI 880 CCATGG C^CATGG
2435 CCATGG
PaeR7I 916 CTCGAG C^TCGAG
1594 CTCGAG
PflMI 1397 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3329 CCAGACTCTGG
PshBI 2042 ATTAAT AT^TAAT
2945 ATTAAT
PvuII 3390 CAGCTG CAG^CTG
3816 CAGCTG
SspBI 82 TGTACA T^GTACA
1783 TGTACA
StuI 1839 AGGCCT AGG^CCT
3149 AGGCCT
Van91I 1397 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3329 CCAGACTCTGG
XhoI 916 CTCGAG C^TCGAG
1594 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SexAI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII