##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspI AviII
BbrPI BglII BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I FseI
FspI HgaI HindIII HpaI KspI
MamI MluI MroI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 4628 CGCG CG^CG
AcyI 4456 GGCGCC G[GA]^CG[CT]C
AflIII 815 ACATGT A^C[GA][CT]GT
AhdI 5294 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1234 CCTCAGG CC^T[ACGT]AGG
AspEI 5294 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 366 GGATCC G^GATCC
BbeI 4456 GGCGCC GGCGC^C
BciVI 1723 GGATAC GTATCC(6/5)
BclI 1061 TGATCA T^GATCA
BglI 4631 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 376 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 376 GCTTAGC GC^T[ACGT]AGC
BsaHI 4456 GGCGCC G[GA]^CG[CT]C
BsmBI 4526 GAGACG CGTCTC(1/5)
Bsp1407I 5281 TGTACA T^GTACA
BspLU11I 815 ACATGT A^CATGT
BsrDI 1100 CATTGC GCAATG(2/0)
BsrFI 4636 GCCGGC [GA]^CCGG[CT]
BsrGI 5281 TGTACA T^GTACA
BstUI 4628 CGCG CG^CG
Bsu36I 1234 CCTCAGG CC^T[ACGT]AGG
CelII 376 GCTTAGC GC^T[ACGT]AGC
Cfr10I 4636 GCCGGC [GA]^CCGG[CT]
DsaI 3685 CCACGG C^C[GA][CT]GG
Eam1105I 5294 GACAGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1234 CCTCAGG CC^T[ACGT]AGG
EcoNI 16 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2883 GAATTC G^AATTC
EcoRV 918 GATATC GAT^ATC
FbaI 1061 TGATCA T^GATCA
HaeII 4456 GGCGCC [GA]GCGC^[CT]
Hin1I 4456 GGCGCC G[GA]^CG[CT]C
KasI 4456 GGCGCC G^GCGCC
MaeII 913 ACGT A^CGT
MfeI 1819 CAATTG C^AATTG
MunI 1819 CAATTG C^AATTG
MvnI 4628 CGCG CG^CG
NaeI 4636 GCCGGC GCC^GGC
NarI 4456 GGCGCC GG^CGCC
NgoMIV 4636 GCCGGC G^CCGGC
PshAI 1146 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 129 GAAGAGC GCTCTTC(1/4)
SexAI 3516 ACCTGGT A^CC[AT]GGT
SfoI 4456 GGCGCC GGC^GCC
SspBI 5281 TGTACA T^GTACA
StuI 806 AGGCCT AGG^CCT
XbaI 3317 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1812 GGTACC G^GTACC
3977 GGTACC
AflII 4207 CTTAAG C^TTAAG
5016 CTTAAG
Asp718I 1812 GGTACC G^GTACC
3977 GGTACC
AvrII 4864 CCTAGG C^CTAGG
5429 CCTAGG
BbsI 5291 GAAGAC GAAGAC(2/6)
1482 GTCTTC
BcgI 2741 CGAGGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3074 CGAGGAGCCTGC
BfrI 4207 CTTAAG C^TTAAG
5016 CTTAAG
BlnI 4864 CCTAGG C^CTAGG
5429 CCTAGG
BpuAI 5291 GAAGAC GAAGAC(2/6)
1482 GTCTTC
BsmI 4922 GAATGC GAATGC(1/-1)
5178 GCATTC
BstEII 1190 GGTCACC G^GT[ACGT]ACC
3389 GGTGACC
BstPI 1190 GGTCACC G^GT[ACGT]ACC
3389 GGTGACC
BstXI 1426 CCACACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4597 CCAGAGTGCTGG
DraI 3725 TTTAAA TTT^AAA
4109 TTTAAA
EcoO65I 1190 GGTCACC G^GT[ACGT]ACC
3389 GGTGACC
EcoT22I 4924 ATGCAT ATGCA^T
5196 ATGCAT
KpnI 1812 GGTACC GGTAC^C
3977 GGTACC
NsiI 4924 ATGCAT ATGCA^T
5196 ATGCAT
PflMI 3998 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5372 CCAACATTTGG
SmaI 3569 CCCGGG CCC^GGG
4404 CCCGGG
SphI 2186 GCATGC GCATG^C
5076 GCATGC
SspI 1662 AATATT AAT^ATT
5333 AATATT
TaqI 4559 TCGA T^CGA
5419 TCGA
TthHB8I 4559 TCGA T^CGA
5419 TCGA
Van91I 3998 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5372 CCAACATTTGG
XmaCI 3569 CCCGGG C^CCGGG
4404 CCCGGG
XmaI 3569 CCCGGG C^CCGGG
4404 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII