##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AspI AvaI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BlnI
BsaBI BsaI BseAI BsiWI BsmI
BsoBI BspEI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CpoI DraIII
DrdI Eco81I EcoRI EcoT22I FbaI
FseI FspI KasI KpnI MamI
MluI MroI NarI NotI NruI
NsiI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1637 GACGTC GACGT^C
AccI 2971 GTCTAC GT^[AC][GT]AC
AflII 3755 CTTAAG C^TTAAG
AhdI 2809 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3204 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 122 AGCGCT AGC^GCT
AseI 3295 ATTAAT AT^TAAT
AsnI 3295 ATTAAT AT^TAAT
AspEI 2809 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2062 TGGCCA TGG^CCA
BfrI 3755 CTTAAG C^TTAAG
BglI 329 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1176 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1176 GCTAAGC GC^T[ACGT]AGC
BsaAI 216 TACGTG [CT]AC^GT[GA]
BsaWI 2659 ACCGGA [AT]^CCGG[AT]
BsmBI 1635 GAGACG CGTCTC(1/5)
BspDI 2361 ATCGAT AT^CGAT
BspMI 2906 ACCTGC ACCTGC(4/8)
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BstAPI 1288 GCAACAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 432 GGTCACC G^GT[ACGT]ACC
BstPI 432 GGTCACC G^GT[ACGT]ACC
CelII 1176 GCTAAGC GC^T[ACGT]AGC
ClaI 2361 ATCGAT AT^CGAT
EagI 37 CGGCCG C^GGCCG
Eam1105I 2809 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 37 CGGCCG C^GGCCG
Eco47III 122 AGCGCT AGC^GCT
Eco52I 37 CGGCCG C^GGCCG
EcoO65I 432 GGTCACC G^GT[ACGT]ACC
HaeII 122 AGCGCT [GA]GCGC^[CT]
HgaI 32 GACGC GACGC(5/10)
HindIII 3691 AAGCTT A^AGCTT
HpaI 3953 GTTAAC GTT^AAC
KspI 135 CCGCGG CCGC^GG
MluNI 2062 TGGCCA TGG^CCA
MscI 2062 TGGCCA TGG^CCA
NcoI 2824 CCATGG C^CATGG
NheI 2516 GCTAGC G^CTAGC
PacI 3292 TTAATTAA TTAAT^TAA
PshBI 3295 ATTAAT AT^TAAT
SacI 418 GAGCTC GAGCT^C
SacII 135 CCGCGG CCGC^GG
XbaI 3365 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 52 CGCG CG^CG
136 CGCG
AcsI 1612 AAATTC [GA]^AATT[CT]
2620 AAATTC
AcyI 32 GACGCC G[GA]^CG[CT]C
1637 GACGTC
ApoI 1612 AAATTC [GA]^AATT[CT]
2620 AAATTC
Asp700I 1608 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1925 GAAGCTGTTC
AspHI 418 GAGCTC G[AT]GC[AT]^C
3452 GTGCTC
BamHI 290 GGATCC G^GATCC
977 GGATCC
BanI 744 GGTGCC G^G[CT][GA]CC
3353 GGTGCC
BbvCI 243 CCTCAGC CCTCAGC(-5/-2)
2151 CCTCAGC
BglII 2779 AGATCT A^GATCT
3277 AGATCT
BpmI 928 CTGGAG CTGGAG(16/14)
2161 CTGGAG
BsaHI 32 GACGCC G[GA]^CG[CT]C
1637 GACGTC
BsgI 508 CTGCAC GTGCAG(16/14)
937 CTGCAC
BsiEI 37 CGGCCG CG[GA][CT]^CG
66 CGACCG
BsiHKAI 418 GAGCTC G[AT]GC[AT]^C
3452 GTGCTC
BspHI 1531 TCATGA T^CATGA
2818 TCATGA
BsrFI 35 GCCGGC [GA]^CCGG[CT]
98 GCCGGC
BstUI 52 CGCG CG^CG
136 CGCG
BstXI 398 CCATCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2824 CCATGGGTGTGG
Cfr10I 35 GCCGGC [GA]^CCGG[CT]
98 GCCGGC
DraI 2580 TTTAAA TTT^AAA
3531 TTTAAA
EcoNI 249 CCTGGAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
828 CCTCTTCCAGG
EcoRV 856 GATATC GAT^ATC
1345 GATATC
Hin1I 32 GACGCC G[GA]^CG[CT]C
1637 GACGTC
MvnI 52 CGCG CG^CG
136 CGCG
NaeI 35 GCCGGC GCC^GGC
98 GCCGGC
NdeI 1580 CATATG CA^TATG
2643 CATATG
NgoMIV 35 GCCGGC G^CCGGC
98 GCCGGC
PstI 2504 CTGCAG CTGCA^G
2683 CTGCAG
RcaI 1531 TCATGA T^CATGA
2818 TCATGA
SapI 1623 GAAGAGC GCTCTTC(1/4)
3238 GCTCTTC
SmlI 703 CTCAAG C^T[CT][GA]AG
3755 CTTAAG
SspI 2951 AATATT AAT^ATT
3026 AATATT
StuI 257 AGGCCT AGG^CCT
1300 AGGCCT
XcmI 620 CCAGGGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2065 CCAAGGACTGCGTGG
XmnI 1608 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1925 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII