##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AspI AviII BcgI BclI BfrI
BglII BsaBI BsaWI BseAI BsiWI
BspEI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI Eam1105I EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
RsrII SacII SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1160 GGTACC G^GTACC
AccI 553 GTCGAC GT^[AC][GT]AC
Alw44I 2775 GTGCAC G^TGCAC
AocI 3327 CCTGAGG CC^T[ACGT]AGG
ApaLI 2775 GTGCAC G^TGCAC
Asp700I 499 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1160 GGTACC G^GTACC
AvrII 3734 CCTAGG C^CTAGG
BbrPI 1891 CACGTG CAC^GTG
BbvCI 1633 CCTCAGC CCTCAGC(-5/-2)
BglI 1454 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3734 CCTAGG C^CTAGG
BsmI 3547 GCATTC GAATGC(1/-1)
BspDI 360 ATCGAT AT^CGAT
BspHI 1441 TCATGA T^CATGA
BspMI 1595 ACCTGC ACCTGC(4/8)
BsrFI 1748 ACCGGC [GA]^CCGG[CT]
BstAPI 2993 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2151 GGTCACC G^GT[ACGT]ACC
BstPI 2151 GGTCACC G^GT[ACGT]ACC
Bsu36I 3327 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1748 ACCGGC [GA]^CCGG[CT]
ClaI 360 ATCGAT AT^CGAT
Eco81I 3327 CCTGAGG CC^T[ACGT]AGG
EcoNI 337 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2151 GGTCACC G^GT[ACGT]ACC
EcoRI 503 GAATTC G^AATTC
EcoRV 1917 GATATC GAT^ATC
HincII 553 GTCGAC GT[CT]^[GA]AC
HindII 553 GTCGAC GT[CT]^[GA]AC
KpnI 1160 GGTACC GGTAC^C
PaeR7I 934 CTCGAG C^TCGAG
PmaCI 1891 CACGTG CAC^GTG
PmlI 1891 CACGTG CAC^GTG
PvuI 178 CGATCG CGAT^CG
RcaI 1441 TCATGA T^CATGA
SalI 553 GTCGAC G^TCGAC
ScaI 1813 AGTACT AGT^ACT
SphI 453 GCATGC GCATG^C
SspI 2524 AATATT AAT^ATT
XhoI 934 CTCGAG C^TCGAG
XmnI 499 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 107 ACATGT A^C[GA][CT]GT
3476 ACATGT
AlwNI 958 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1200 CAGACTCTG
AvaI 934 CTCGAG C^[CT]CG[GA]G
2659 CCCGAG
BalI 687 TGGCCA TGG^CCA
1086 TGGCCA
BamHI 349 GGATCC G^GATCC
2273 GGATCC
BbeI 443 GGCGCC GGCGC^C
1583 GGCGCC
BsaI 647 GAGACC GGTCTC(1/5)
667 GGTCTC
BsmBI 461 GAGACG CGTCTC(1/5)
3052 GAGACG
BsoBI 934 CTCGAG C^[CT]CG[GA]G
2659 CCCGAG
BspLU11I 107 ACATGT A^CATGT
3476 ACATGT
BsrBI 890 GAGCGG CCGCTC(-3/-3)
2171 GAGCGG
BsrDI 2114 CATTGC GCAATG(2/0)
3589 GCAATG
BstXI 195 CCAGTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1855 CCATGTAGGTGG
DraIII 341 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1600 CACCCAGTG
EagI 52 CGGCCG C^GGCCG
1244 CGGCCG
EclXI 52 CGGCCG C^GGCCG
1244 CGGCCG
Eco52I 52 CGGCCG C^GGCCG
1244 CGGCCG
KasI 443 GGCGCC G^GCGCC
1583 GGCGCC
MluNI 687 TGGCCA TGG^CCA
1086 TGGCCA
MscI 687 TGGCCA TGG^CCA
1086 TGGCCA
NarI 443 GGCGCC GG^CGCC
1583 GGCGCC
PflMI 2292 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3129 CCAACCCCTGG
PpuMI 2088 GGGACCC [GA]G^G[AT]CC[CT]
2844 GGGACCT
PvuII 1700 CAGCTG CAG^CTG
2280 CAGCTG
SfoI 443 GGCGCC GGC^GCC
1583 GGCGCC
Van91I 2292 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3129 CCAACCCCTGG
XcmI 855 CCATGTCACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3388 CCAAATCTTCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI Aor51HI ApaI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BciVI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII