##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       BbeI        
BcgI        BfrI        BglII       BsaBI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BssHII      
Bst1107I    BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FseI        
FspI        KasI        KpnI        KspI        MamI        
MluI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshBI       PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          311  GTCTAC               GT^[AC][GT]AC
AccII          53  CGCG                 CG^CG
AccIII        841  TCCGGA               T^CCGGA
AclI         2298  AACGTT               AA^CGTT
AocI         3818  CCTGAGG              CC^T[ACGT]AGG
AvrII        4637  CCTAGG               C^CTAGG
BalI         1636  TGGCCA               TGG^CCA
BanI          670  GGCACC               G^G[CT][GA]CC
BbvCI        3518  CCTCAGC              CCTCAGC(-5/-2)
BciVI        5368  GGATAC               GTATCC(6/5)
BlnI         4637  CCTAGG               C^CTAGG
BlpI           14  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       14  GCTGAGC              GC^T[ACGT]AGC
BsaI         3930  GGTCTC               GGTCTC(1/5)
BsaWI         841  TCCGGA               [AT]^CCGG[AT]
BseAI         841  TCCGGA               T^CCGGA
Bsp1407I      980  TGTACA               T^GTACA
BspEI         841  TCCGGA               T^CCGGA
BspLU11I     4489  ACATGT               A^CATGT
BsrFI        2287  GCCGGT               [GA]^CCGG[CT]
BsrGI         980  TGTACA               T^GTACA
BssSI        1781  CACGAG               CACGAG(-5/-1)
BstAPI       3136  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4562  TTCGAA               TT^CGAA
BstEII       3347  GGTGACC              G^GT[ACGT]ACC
BstPI        3347  GGTGACC              G^GT[ACGT]ACC
BstUI          53  CGCG                 CG^CG
Bsu36I       3818  CCTGAGG              CC^T[ACGT]AGG
CelII          14  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2287  GCCGGT               [GA]^CCGG[CT]
DraI         4318  TTTAAA               TTT^AAA
DrdI         1214  GACTTCGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2923  CCATGG               C^C[GA][CT]GG
EaeI         1636  TGGCCA               [CT]^GGCC[GA]
Eco81I       3818  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      3347  GGTGACC              G^GT[ACGT]ACC
EcoRV        2961  GATATC               GAT^ATC
HaeII        1138  GGCGCT               [GA]GCGC^[CT]
HpaI         2070  GTTAAC               GTT^AAC
MfeI         5215  CAATTG               C^AATTG
MluNI        1636  TGGCCA               TGG^CCA
MroI          841  TCCGGA               T^CCGGA
MscI         1636  TGGCCA               TGG^CCA
MunI         5215  CAATTG               C^AATTG
MvnI           53  CGCG                 CG^CG
NcoI         2923  CCATGG               C^CATGG
NspV         4562  TTCGAA               TT^CGAA
PshAI         548  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2298  AACGTT               AA^CGTT
PvuII         349  CAGCTG               CAG^CTG
ScaI         1811  AGTACT               AGT^ACT
SexAI        3423  ACCTGGT              A^CC[AT]GGT
SfuI         4562  TTCGAA               TT^CGAA
SspBI         980  TGTACA               T^GTACA
StuI         3822  AGGCCT               AGG^CCT
XbaI         2888  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII         654  GACGTC               GACGT^C
             1902  GACGTC              
AcyI          654  GACGTC               G[GA]^CG[CT]C
             1902  GACGTC              
AflIII       4350  ACGTGT               A^C[GA][CT]GT
             4489  ACATGT              
AlwNI        4305  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5247  CAGACCCTG           
BamHI         458  GGATCC               G^GATCC
             2237  GGATCC              
BbrPI        2607  CACGTG               CAC^GTG
             5485  CACGTG              
BpmI         1249  CTCCAG               CTGGAG(16/14)
             2412  CTCCAG              
BsaHI         654  GACGTC               G[GA]^CG[CT]C
             1902  GACGTC              
BsgI          289  CTGCAC               GTGCAG(16/14)
             2778  GTGCAG              
BspHI         335  TCATGA               T^CATGA
              784  TCATGA              
BsrBI          25  GAGCGG               CCGCTC(-3/-3)
              167  GAGCGG              
BstXI         188  CCACAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5480  CCAACCACGTGG        
CfoI         1139  GCGC                 GCG^C
             3374  GCGC                
DraIII        248  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4817  CACTCAGTG           
EcoRI        1877  GAATTC               G^AATTC
             4655  GAATTC              
EcoT22I       445  ATGCAT               ATGCA^T
             1700  ATGCAT              
HgaI         2603  GACGC                GACGC(5/10)
             3523  GCGTC               
HhaI         1139  GCGC                 GCG^C
             3374  GCGC                
Hin1I         654  GACGTC               G[GA]^CG[CT]C
             1902  GACGTC              
HinP1I       1139  GCGC                 G^CGC
             3374  GCGC                
HincII       2070  GTTAAC               GT[CT]^[GA]AC
             4103  GTCAAC              
HindII       2070  GTTAAC               GT[CT]^[GA]AC
             4103  GTCAAC              
MspA1I        349  CAGCTG               C[AC]G^C[GT]G
             1917  CCGCTG              
NdeI          919  CATATG               CA^TATG
             2034  CATATG              
NsiI          445  ATGCAT               ATGCA^T
             1700  ATGCAT              
PmaCI        2607  CACGTG               CAC^GTG
             5485  CACGTG              
PmlI         2607  CACGTG               CAC^GTG
             5485  CACGTG              
PpuMI         810  AGGTCCT              [GA]G^G[AT]CC[CT]
             3892  GGGTCCT             
RcaI          335  TCATGA               T^CATGA
              784  TCATGA              
SapI         3857  GAAGAGC              GCTCTTC(1/4)
             2518  GCTCTTC             
SmaI         3682  CCCGGG               CCC^GGG
             4297  CCCGGG              
XmaCI        3682  CCCGGG               C^CCGGG
             4297  CCCGGG              
XmaI         3682  CCCGGG               C^CCGGG
             4297  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AspI        AvaI        
AvaII       BanII       BbsI        BbvI        BclI        
BcnI        BfaI        BglI        BmyI        BpuAI       
BsaAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmnI