################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BbeI BcgI BfrI BglII BsaBI BsiEI BsiWI BsmBI BsmI BspDI BssHII Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI KasI KpnI KspI MamI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 311 GTCTAC GT^[AC][GT]AC AccII 53 CGCG CG^CG AccIII 841 TCCGGA T^CCGGA AclI 2298 AACGTT AA^CGTT AocI 3818 CCTGAGG CC^T[ACGT]AGG AvrII 4637 CCTAGG C^CTAGG BalI 1636 TGGCCA TGG^CCA BanI 670 GGCACC G^G[CT][GA]CC BbvCI 3518 CCTCAGC CCTCAGC(-5/-2) BciVI 5368 GGATAC GTATCC(6/5) BlnI 4637 CCTAGG C^CTAGG BlpI 14 GCTGAGC GC^T[ACGT]AGC Bpu1102I 14 GCTGAGC GC^T[ACGT]AGC BsaI 3930 GGTCTC GGTCTC(1/5) BsaWI 841 TCCGGA [AT]^CCGG[AT] BseAI 841 TCCGGA T^CCGGA Bsp1407I 980 TGTACA T^GTACA BspEI 841 TCCGGA T^CCGGA BspLU11I 4489 ACATGT A^CATGT BsrFI 2287 GCCGGT [GA]^CCGG[CT] BsrGI 980 TGTACA T^GTACA BssSI 1781 CACGAG CACGAG(-5/-1) BstAPI 3136 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4562 TTCGAA TT^CGAA BstEII 3347 GGTGACC G^GT[ACGT]ACC BstPI 3347 GGTGACC G^GT[ACGT]ACC BstUI 53 CGCG CG^CG Bsu36I 3818 CCTGAGG CC^T[ACGT]AGG CelII 14 GCTGAGC GC^T[ACGT]AGC Cfr10I 2287 GCCGGT [GA]^CCGG[CT] DraI 4318 TTTAAA TTT^AAA DrdI 1214 GACTTCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2923 CCATGG C^C[GA][CT]GG EaeI 1636 TGGCCA [CT]^GGCC[GA] Eco81I 3818 CCTGAGG CC^T[ACGT]AGG EcoO65I 3347 GGTGACC G^GT[ACGT]ACC EcoRV 2961 GATATC GAT^ATC HaeII 1138 GGCGCT [GA]GCGC^[CT] HpaI 2070 GTTAAC GTT^AAC MfeI 5215 CAATTG C^AATTG MluNI 1636 TGGCCA TGG^CCA MroI 841 TCCGGA T^CCGGA MscI 1636 TGGCCA TGG^CCA MunI 5215 CAATTG C^AATTG MvnI 53 CGCG CG^CG NcoI 2923 CCATGG C^CATGG NspV 4562 TTCGAA TT^CGAA PshAI 548 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2298 AACGTT AA^CGTT PvuII 349 CAGCTG CAG^CTG ScaI 1811 AGTACT AGT^ACT SexAI 3423 ACCTGGT A^CC[AT]GGT SfuI 4562 TTCGAA TT^CGAA SspBI 980 TGTACA T^GTACA StuI 3822 AGGCCT AGG^CCT XbaI 2888 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 654 GACGTC GACGT^C 1902 GACGTC AcyI 654 GACGTC G[GA]^CG[CT]C 1902 GACGTC AflIII 4350 ACGTGT A^C[GA][CT]GT 4489 ACATGT AlwNI 4305 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 5247 CAGACCCTG BamHI 458 GGATCC G^GATCC 2237 GGATCC BbrPI 2607 CACGTG CAC^GTG 5485 CACGTG BpmI 1249 CTCCAG CTGGAG(16/14) 2412 CTCCAG BsaHI 654 GACGTC G[GA]^CG[CT]C 1902 GACGTC BsgI 289 CTGCAC GTGCAG(16/14) 2778 GTGCAG BspHI 335 TCATGA T^CATGA 784 TCATGA BsrBI 25 GAGCGG CCGCTC(-3/-3) 167 GAGCGG BstXI 188 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5480 CCAACCACGTGG CfoI 1139 GCGC GCG^C 3374 GCGC DraIII 248 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 4817 CACTCAGTG EcoRI 1877 GAATTC G^AATTC 4655 GAATTC EcoT22I 445 ATGCAT ATGCA^T 1700 ATGCAT HgaI 2603 GACGC GACGC(5/10) 3523 GCGTC HhaI 1139 GCGC GCG^C 3374 GCGC Hin1I 654 GACGTC G[GA]^CG[CT]C 1902 GACGTC HinP1I 1139 GCGC G^CGC 3374 GCGC HincII 2070 GTTAAC GT[CT]^[GA]AC 4103 GTCAAC HindII 2070 GTTAAC GT[CT]^[GA]AC 4103 GTCAAC MspA1I 349 CAGCTG C[AC]G^C[GT]G 1917 CCGCTG NdeI 919 CATATG CA^TATG 2034 CATATG NsiI 445 ATGCAT ATGCA^T 1700 ATGCAT PmaCI 2607 CACGTG CAC^GTG 5485 CACGTG PmlI 2607 CACGTG CAC^GTG 5485 CACGTG PpuMI 810 AGGTCCT [GA]G^G[AT]CC[CT] 3892 GGGTCCT RcaI 335 TCATGA T^CATGA 784 TCATGA SapI 3857 GAAGAGC GCTCTTC(1/4) 2518 GCTCTTC SmaI 3682 CCCGGG CCC^GGG 4297 CCCGGG XmaCI 3682 CCCGGG C^CCGGG 4297 CCCGGG XmaI 3682 CCCGGG C^CCGGG 4297 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AspI AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BglI BmyI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI