##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BcgI BfrI BglII BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI KasI KpnI KspI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 311 GTCTAC GT^[AC][GT]AC
AccII 53 CGCG CG^CG
AccIII 841 TCCGGA T^CCGGA
AclI 2298 AACGTT AA^CGTT
AocI 3818 CCTGAGG CC^T[ACGT]AGG
AvrII 4637 CCTAGG C^CTAGG
BalI 1636 TGGCCA TGG^CCA
BanI 670 GGCACC G^G[CT][GA]CC
BbvCI 3518 CCTCAGC CCTCAGC(-5/-2)
BciVI 5368 GGATAC GTATCC(6/5)
BlnI 4637 CCTAGG C^CTAGG
BlpI 14 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 14 GCTGAGC GC^T[ACGT]AGC
BsaI 3930 GGTCTC GGTCTC(1/5)
BsaWI 841 TCCGGA [AT]^CCGG[AT]
BseAI 841 TCCGGA T^CCGGA
Bsp1407I 980 TGTACA T^GTACA
BspEI 841 TCCGGA T^CCGGA
BspLU11I 4489 ACATGT A^CATGT
BsrFI 2287 GCCGGT [GA]^CCGG[CT]
BsrGI 980 TGTACA T^GTACA
BssSI 1781 CACGAG CACGAG(-5/-1)
BstAPI 3136 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4562 TTCGAA TT^CGAA
BstEII 3347 GGTGACC G^GT[ACGT]ACC
BstPI 3347 GGTGACC G^GT[ACGT]ACC
BstUI 53 CGCG CG^CG
Bsu36I 3818 CCTGAGG CC^T[ACGT]AGG
CelII 14 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2287 GCCGGT [GA]^CCGG[CT]
DraI 4318 TTTAAA TTT^AAA
DrdI 1214 GACTTCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2923 CCATGG C^C[GA][CT]GG
EaeI 1636 TGGCCA [CT]^GGCC[GA]
Eco81I 3818 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3347 GGTGACC G^GT[ACGT]ACC
EcoRV 2961 GATATC GAT^ATC
HaeII 1138 GGCGCT [GA]GCGC^[CT]
HpaI 2070 GTTAAC GTT^AAC
MfeI 5215 CAATTG C^AATTG
MluNI 1636 TGGCCA TGG^CCA
MroI 841 TCCGGA T^CCGGA
MscI 1636 TGGCCA TGG^CCA
MunI 5215 CAATTG C^AATTG
MvnI 53 CGCG CG^CG
NcoI 2923 CCATGG C^CATGG
NspV 4562 TTCGAA TT^CGAA
PshAI 548 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2298 AACGTT AA^CGTT
PvuII 349 CAGCTG CAG^CTG
ScaI 1811 AGTACT AGT^ACT
SexAI 3423 ACCTGGT A^CC[AT]GGT
SfuI 4562 TTCGAA TT^CGAA
SspBI 980 TGTACA T^GTACA
StuI 3822 AGGCCT AGG^CCT
XbaI 2888 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 654 GACGTC GACGT^C
1902 GACGTC
AcyI 654 GACGTC G[GA]^CG[CT]C
1902 GACGTC
AflIII 4350 ACGTGT A^C[GA][CT]GT
4489 ACATGT
AlwNI 4305 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
5247 CAGACCCTG
BamHI 458 GGATCC G^GATCC
2237 GGATCC
BbrPI 2607 CACGTG CAC^GTG
5485 CACGTG
BpmI 1249 CTCCAG CTGGAG(16/14)
2412 CTCCAG
BsaHI 654 GACGTC G[GA]^CG[CT]C
1902 GACGTC
BsgI 289 CTGCAC GTGCAG(16/14)
2778 GTGCAG
BspHI 335 TCATGA T^CATGA
784 TCATGA
BsrBI 25 GAGCGG CCGCTC(-3/-3)
167 GAGCGG
BstXI 188 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5480 CCAACCACGTGG
CfoI 1139 GCGC GCG^C
3374 GCGC
DraIII 248 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4817 CACTCAGTG
EcoRI 1877 GAATTC G^AATTC
4655 GAATTC
EcoT22I 445 ATGCAT ATGCA^T
1700 ATGCAT
HgaI 2603 GACGC GACGC(5/10)
3523 GCGTC
HhaI 1139 GCGC GCG^C
3374 GCGC
Hin1I 654 GACGTC G[GA]^CG[CT]C
1902 GACGTC
HinP1I 1139 GCGC G^CGC
3374 GCGC
HincII 2070 GTTAAC GT[CT]^[GA]AC
4103 GTCAAC
HindII 2070 GTTAAC GT[CT]^[GA]AC
4103 GTCAAC
MspA1I 349 CAGCTG C[AC]G^C[GT]G
1917 CCGCTG
NdeI 919 CATATG CA^TATG
2034 CATATG
NsiI 445 ATGCAT ATGCA^T
1700 ATGCAT
PmaCI 2607 CACGTG CAC^GTG
5485 CACGTG
PmlI 2607 CACGTG CAC^GTG
5485 CACGTG
PpuMI 810 AGGTCCT [GA]G^G[AT]CC[CT]
3892 GGGTCCT
RcaI 335 TCATGA T^CATGA
784 TCATGA
SapI 3857 GAAGAGC GCTCTTC(1/4)
2518 GCTCTTC
SmaI 3682 CCCGGG CCC^GGG
4297 CCCGGG
XmaCI 3682 CCCGGG C^CCGGG
4297 CCCGGG
XmaI 3682 CCCGGG C^CCGGG
4297 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AspI AvaI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BglI BmyI BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI