##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AcsI AgeI ApoI AscI
AvrII BamHI BbeI BbvCI BcgI
BclI BlnI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DrdI EcoRI EcoRV
EcoT22I FbaI FseI KasI KspI
MfeI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PaeR7I PflMI PinAI PmeI PshAI
PvuI RcaI RsrII SacII SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3067 GACGTC GACGT^C
Acc65I 2352 GGTACC G^GTACC
AclI 2617 AACGTT AA^CGTT
AflII 2407 CTTAAG C^TTAAG
AhdI 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2717 AGCGCT AGC^GCT
AseI 3839 ATTAAT AT^TAAT
AsnI 3839 ATTAAT AT^TAAT
Asp700I 3618 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2352 GGTACC G^GTACC
AspEI 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2858 TGCGCA TGC^GCA
BbrPI 661 CACGTG CAC^GTG
BciVI 1713 GTATCC GTATCC(6/5)
BfrI 2407 CTTAAG C^TTAAG
BglI 2135 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 661 CACGTG [CT]AC^GT[GA]
BsaBI 3366 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 710 GCATTC GAATGC(1/-1)
BsrDI 2051 GCAATG GCAATG(2/0)
BstEII 2754 GGTGACC G^GT[ACGT]ACC
BstPI 2754 GGTGACC G^GT[ACGT]ACC
DraI 4003 TTTAAA TTT^AAA
EagI 2554 CGGCCG C^GGCCG
Eam1105I 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2554 CGGCCG C^GGCCG
Eco47III 2717 AGCGCT AGC^GCT
Eco52I 2554 CGGCCG C^GGCCG
EcoO65I 2754 GGTGACC G^GT[ACGT]ACC
FspI 2858 TGCGCA TGC^GCA
HindIII 3061 AAGCTT A^AGCTT
HpaI 4090 GTTAAC GTT^AAC
KpnI 2352 GGTACC GGTAC^C
MamI 3366 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2459 ACGCGT A^CGCGT
PacI 4177 TTAATTAA TTAAT^TAA
PflFI 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 661 CACGTG CAC^GTG
PmlI 661 CACGTG CAC^GTG
PpuMI 3050 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3839 ATTAAT AT^TAAT
Psp1406I 2617 AACGTT AA^CGTT
SacI 2920 GAGCTC GAGCT^C
SexAI 3053 ACCTGGT A^CC[AT]GGT
StuI 2261 AGGCCT AGG^CCT
Tth111I 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3934 TCTAGA T^CTAGA
XmnI 3618 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
BglII 1151 AGATCT A^GATCT
3590 AGATCT
BlpI 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
Bpu1102I 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
BsaI 2182 GAGACC GGTCTC(1/5)
3265 GAGACC
BsaWI 1470 TCCGGT [AT]^CCGG[AT]
4216 ACCGGA
BspLU11I 1144 ACATGT A^CATGT
2696 ACATGT
Bsu36I 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
CelII 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
EarI 340 CTCTTC CTCTTC(1/4)
279 GAAGAG
Eco81I 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
EcoNI 198 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1989 CCTGTGTCAGG
Ksp632I 340 CTCTTC CTCTTC(1/4)
279 GAAGAG
NaeI 3097 GCCGGC GCC^GGC
3172 GCCGGC
NgoMIV 3097 GCCGGC G^CCGGC
3172 GCCGGC
PvuII 682 CAGCTG CAG^CTG
3824 CAGCTG
SalI 733 GTCGAC G^TCGAC
1884 GTCGAC
SphI 2399 GCATGC GCATG^C
2894 GCATGC
SspI 3836 AATATT AAT^ATT
4158 AATATT
TfiI 10 GAATC G^A[AT]TC
2526 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII