##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI AocI AscI AspEI AviII
BbeI BglII BsaHI BseAI BseRI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BstEII
BstPI Bsu36I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI Hin1I KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI PaeR7I PinAI
PmeI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1375 GGTACC G^GTACC
AccII 4089 CGCG CG^CG
AflII 3256 CTTAAG C^TTAAG
AflIII 643 ACGTGT A^C[GA][CT]GT
Alw44I 2720 GTGCAC G^TGCAC
AlwNI 1642 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 994 AGCGCT AGC^GCT
ApaI 1213 GGGCCC GGGCC^C
ApaLI 2720 GTGCAC G^TGCAC
Asp700I 2636 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1375 GGTACC G^GTACC
AvaI 2035 CCCGAG C^[CT]CG[GA]G
AvrII 2998 CCTAGG C^CTAGG
BbrPI 1620 CACGTG CAC^GTG
BcgI 3666 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1402 GGATAC GTATCC(6/5)
BcnI 1327 CCGGG CC^[GC]GG
BfrI 3256 CTTAAG C^TTAAG
BlnI 2998 CCTAGG C^CTAGG
BlpI 2576 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2576 GCTGAGC GC^T[ACGT]AGC
BsaAI 1620 CACGTG [CT]AC^GT[GA]
BsaBI 2055 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1633 TCCGGT [AT]^CCGG[AT]
BsmBI 640 GAGACG CGTCTC(1/5)
BsmI 26 GAATGC GAATGC(1/-1)
BsoBI 2035 CCCGAG C^[CT]CG[GA]G
BssSI 1356 CACGAG CACGAG(-5/-1)
BstBI 521 TTCGAA TT^CGAA
BstUI 4089 CGCG CG^CG
CelII 2576 GCTGAGC GC^T[ACGT]AGC
DraIII 462 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3616 GACACGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 994 AGCGCT AGC^GCT
EcoT22I 362 ATGCAT ATGCA^T
HaeII 994 AGCGCT [GA]GCGC^[CT]
HgaI 1346 GACGC GACGC(5/10)
HpaI 4134 GTTAAC GTT^AAC
KpnI 1375 GGTACC GGTAC^C
MamI 2055 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 4089 CGCG CG^CG
NciI 1327 CCGGG CC^[GC]GG
NcoI 3885 CCATGG C^CATGG
NdeI 615 CATATG CA^TATG
NsiI 362 ATGCAT ATGCA^T
NspV 521 TTCGAA TT^CGAA
PacI 476 TTAATTAA TTAAT^TAA
PflMI 3218 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1620 CACGTG CAC^GTG
PmlI 1620 CACGTG CAC^GTG
SacI 851 GAGCTC GAGCT^C
ScaI 1460 AGTACT AGT^ACT
SexAI 442 ACCAGGT A^CC[AT]GGT
SfiI 1205 GGCCAAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 521 TTCGAA TT^CGAA
SpeI 859 ACTAGT A^CTAGT
SspI 4158 AATATT AAT^ATT
Van91I 3218 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3148 TCTAGA T^CTAGA
XcmI 3876 CCACTACATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2636 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 3154 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3316 GACAGAGTC
BanI 446 GGTGCC G^G[CT][GA]CC
1375 GGTACC
BclI 3838 TGATCA T^GATCA
4484 TGATCA
BglI 1206 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1644 GCCCCTGTGGC
BsgI 3185 GTGCAG GTGCAG(16/14)
3209 GTGCAG
Bsp1407I 2353 TGTACA T^GTACA
2378 TGTACA
BspMI 1178 GCAGGT ACCTGC(4/8)
4463 GCAGGT
BsrGI 2353 TGTACA T^GTACA
2378 TGTACA
BstAPI 1178 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3373 GCAATCTCTGC
EarI 1257 GAAGAG CTCTTC(1/4)
3088 GAAGAG
FbaI 3838 TGATCA T^GATCA
4484 TGATCA
HapII 1327 CCGG C^CGG
1634 CCGG
HpaII 1327 CCGG C^CGG
1634 CCGG
Ksp632I 1257 GAAGAG CTCTTC(1/4)
3088 GAAGAG
MspI 1327 CCGG C^CGG
1634 CCGG
NheI 703 GCTAGC G^CTAGC
1735 GCTAGC
PflFI 3154 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3316 GACAGAGTC
PpuMI 2807 AGGTCCC [GA]G^G[AT]CC[CT]
4069 AGGACCC
PvuII 2255 CAGCTG CAG^CTG
2715 CAGCTG
SphI 3287 GCATGC GCATG^C
3443 GCATGC
SspBI 2353 TGTACA T^GTACA
2378 TGTACA
Tth111I 3154 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3316 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BalI BamHI BanII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI Bst1107I BstF5I BstNI
BstXI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII