##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII AvrII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstZ17I Bsu36I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI HpaI KpnI
KspI MamI MluI MroI NdeI
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI Sse8387I
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 2705 GTAGAC GT^[AC][GT]AC
AccII 682 CGCG CG^CG
AclI 2080 AACGTT AA^CGTT
AflII 4020 CTTAAG C^TTAAG
AseI 35 ATTAAT AT^TAAT
AsnI 35 ATTAAT AT^TAAT
AspI 1011 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2366 GGCGCC GGCGC^C
BbrPI 1815 CACGTG CAC^GTG
BbvCI 1178 CCTCAGC CCTCAGC(-5/-2)
BcgI 1887 CGAAAAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3840 GTATCC GTATCC(6/5)
BclI 1504 TGATCA T^GATCA
BfrI 4020 CTTAAG C^TTAAG
BglI 620 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 752 AGATCT A^GATCT
BlpI 3282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3282 GCTGAGC GC^T[ACGT]AGC
BsaAI 1815 CACGTG [CT]AC^GT[GA]
Bsp1407I 2933 TGTACA T^GTACA
BspHI 3203 TCATGA T^CATGA
BsrFI 1963 GCCGGC [GA]^CCGG[CT]
BsrGI 2933 TGTACA T^GTACA
BstAPI 1294 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 833 GGTGACC G^GT[ACGT]ACC
BstPI 833 GGTGACC G^GT[ACGT]ACC
BstUI 682 CGCG CG^CG
CelII 3282 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1963 GCCGGC [GA]^CCGG[CT]
DraIII 1766 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4035 CCTAATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 833 GGTGACC G^GT[ACGT]ACC
EcoRI 2071 GAATTC G^AATTC
FbaI 1504 TGATCA T^GATCA
HincII 1401 GTCAAC GT[CT]^[GA]AC
HindII 1401 GTCAAC GT[CT]^[GA]AC
HindIII 2886 AAGCTT A^AGCTT
KasI 2366 GGCGCC G^GCGCC
MfeI 131 CAATTG C^AATTG
MunI 131 CAATTG C^AATTG
MvnI 682 CGCG CG^CG
NaeI 1963 GCCGGC GCC^GGC
NarI 2366 GGCGCC GG^CGCC
NgoMIV 1963 GCCGGC G^CCGGC
PflFI 1011 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1815 CACGTG CAC^GTG
PmlI 1815 CACGTG CAC^GTG
PshBI 35 ATTAAT AT^TAAT
Psp1406I 2080 AACGTT AA^CGTT
RcaI 3203 TCATGA T^CATGA
SacI 1761 GAGCTC GAGCT^C
SfoI 2366 GGCGCC GGC^GCC
SmaI 1321 CCCGGG CCC^GGG
SspBI 2933 TGTACA T^GTACA
StuI 3528 AGGCCT AGG^CCT
Tth111I 1011 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1321 CCCGGG C^CCGGG
XmaI 1321 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 516 GGCGTC G[GA]^CG[CT]C
2366 GGCGCC
AflIII 2434 ACATGT A^C[GA][CT]GT
3847 ACATGT
ApaI 713 GGGCCC GGGCC^C
1223 GGGCCC
AvaI 1321 CCCGGG C^[CT]CG[GA]G
1954 CCCGAG
BamHI 178 GGATCC G^GATCC
462 GGATCC
BsaHI 516 GGCGTC G[GA]^CG[CT]C
2366 GGCGCC
BsoBI 1321 CCCGGG C^[CT]CG[GA]G
1954 CCCGAG
BspLU11I 2434 ACATGT A^CATGT
3847 ACATGT
BstBI 1822 TTCGAA TT^CGAA
1915 TTCGAA
BstXI 3330 CCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4125 CCAGGTTAATGG
EcoT22I 3154 ATGCAT ATGCA^T
3353 ATGCAT
HaeII 1464 AGCGCC [GA]GCGC^[CT]
2366 GGCGCC
Hin1I 516 GGCGTC G[GA]^CG[CT]C
2366 GGCGCC
NsiI 3154 ATGCAT ATGCA^T
3353 ATGCAT
NspV 1822 TTCGAA TT^CGAA
1915 TTCGAA
SexAI 1278 ACCTGGT A^CC[AT]GGT
4124 ACCAGGT
SfuI 1822 TTCGAA TT^CGAA
1915 TTCGAA
SwaI 2255 ATTTAAAT ATTT^AAAT
2842 ATTTAAAT
XbaI 2206 TCTAGA T^CTAGA
3065 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI