##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
BssSI BstEII BstPI Bsu36I CelII
Cfr10I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
FbaI FseI HaeII HgaI Hin1I
KasI KpnI MamI MluI MluNI
MroI MscI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 3443 GTATAC GT^[AC][GT]AC
AflII 3477 CTTAAG C^TTAAG
AseI 2995 ATTAAT AT^TAAT
AsnI 2995 ATTAAT AT^TAAT
Asp700I 34 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 324 TGCGCA TGC^GCA
BciVI 518 GTATCC GTATCC(6/5)
BfrI 3477 CTTAAG C^TTAAG
BglII 2976 AGATCT A^GATCT
BsaI 176 GGTCTC GGTCTC(1/5)
BsiEI 57 CGGCCG CG[GA][CT]^CG
Bsp1407I 1870 TGTACA T^GTACA
BspMI 916 ACCTGC ACCTGC(4/8)
BsrBI 5235 GAGCGG CCGCTC(-3/-3)
BsrGI 1870 TGTACA T^GTACA
Bst1107I 3443 GTATAC GTA^TAC
BstBI 1701 TTCGAA TT^CGAA
BstXI 2665 CCATATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3443 GTATAC GTA^TAC
DraIII 540 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 57 CGGCCG [CT]^GGCC[GA]
EagI 57 CGGCCG C^GGCCG
EarI 2258 GAAGAG CTCTTC(1/4)
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
EcoT22I 2999 ATGCAT ATGCA^T
FspI 324 TGCGCA TGC^GCA
HpaI 998 GTTAAC GTT^AAC
Ksp632I 2258 GAAGAG CTCTTC(1/4)
KspI 60 CCGCGG CCGC^GG
NsiI 2999 ATGCAT ATGCA^T
NspV 1701 TTCGAA TT^CGAA
PflMI 2618 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2995 ATTAAT AT^TAAT
SacII 60 CCGCGG CCGC^GG
SapI 2258 GAAGAGC GCTCTTC(1/4)
SfuI 1701 TTCGAA TT^CGAA
SmaI 2557 CCCGGG CCC^GGG
SspBI 1870 TGTACA T^GTACA
Van91I 2618 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3241 TCTAGA T^CTAGA
XmaCI 2557 CCCGGG C^CCGGG
XmaI 2557 CCCGGG C^CCGGG
XmnI 34 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AclI 658 AACGTT AA^CGTT
4907 AACGTT
AlwNI 2883 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3319 CAGCTGCTG
ApaI 2512 GGGCCC GGGCC^C
3910 GGGCCC
AvaI 2557 CCCGGG C^[CT]CG[GA]G
2868 CTCGGG
BanI 109 GGTGCC G^G[CT][GA]CC
907 GGCACC
BsaAI 1944 CACGTA [CT]AC^GT[GA]
4023 TACGTG
BsmAI 177 GTCTC GTCTC(1/5)
2866 GTCTC
BsoBI 2557 CCCGGG C^[CT]CG[GA]G
2868 CTCGGG
BspLU11I 1483 ACATGT A^CATGT
1873 ACATGT
BstAPI 1075 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2731 GCAAAACATGC
BstYI 2976 AGATCT [GA]^GATC[CT]
3996 AGATCC
MfeI 183 CAATTG C^AATTG
3052 CAATTG
MflI 2976 AGATCT [GA]^GATC[CT]
3996 AGATCC
MunI 183 CAATTG C^AATTG
3052 CAATTG
NcoI 198 CCATGG C^CATGG
2850 CCATGG
NdeI 3264 CATATG CA^TATG
3839 CATATG
PpuMI 1336 GGGACCT [GA]G^G[AT]CC[CT]
3482 GGGTCCT
Psp1406I 658 AACGTT AA^CGTT
4907 AACGTT
PstI 1712 CTGCAG CTGCA^G
2091 CTGCAG
PvuII 2883 CAGCTG CAG^CTG
3319 CAGCTG
SphI 879 GCATGC GCATG^C
2263 GCATGC
SspI 1799 AATATT AAT^ATT
5131 AATATT
XhoII 2976 AGATCT [GA]^GATC[CT]
3996 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI