##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII BcgI
BclI BfrI BsaBI BsiWI BspDI
Bst1107I BstZ17I ClaI CpoI Eam1105I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII MamI MluI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SapI SbfI ScaI SexAI
SfiI SgrAI SpeI SphI Sse8387I
SspI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1123 GACGTC GACGT^C
Acc65I 330 GGTACC G^GTACC
AccIII 506 TCCGGA T^CCGGA
AocI 3761 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1424 AGCGCT AGC^GCT
Asp718I 330 GGTACC G^GTACC
AvrII 3906 CCTAGG C^CTAGG
BbeI 3757 GGCGCC GGCGC^C
BglII 1559 AGATCT A^GATCT
BlnI 3906 CCTAGG C^CTAGG
BseAI 506 TCCGGA T^CCGGA
BsmBI 828 GAGACG CGTCTC(1/5)
BspEI 506 TCCGGA T^CCGGA
BspHI 2046 TCATGA T^CATGA
BspLU11I 3562 ACATGT A^CATGT
BssHII 1359 GCGCGC G^CGCGC
BstBI 1702 TTCGAA TT^CGAA
BstEII 457 GGTCACC G^GT[ACGT]ACC
BstPI 457 GGTCACC G^GT[ACGT]ACC
BstXI 3003 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3761 CCTGAGG CC^T[ACGT]AGG
DraIII 3771 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1493 CGGCCG C^GGCCG
EclXI 1493 CGGCCG C^GGCCG
Eco47III 1424 AGCGCT AGC^GCT
Eco52I 1493 CGGCCG C^GGCCG
Eco81I 3761 CCTGAGG CC^T[ACGT]AGG
EcoO65I 457 GGTCACC G^GT[ACGT]ACC
FseI 1494 GGCCGGCC GGCCGG^CC
HpaI 813 GTTAAC GTT^AAC
KasI 3757 GGCGCC G^GCGCC
KpnI 330 GGTACC GGTAC^C
MfeI 2033 CAATTG C^AATTG
MroI 506 TCCGGA T^CCGGA
MunI 2033 CAATTG C^AATTG
NarI 3757 GGCGCC GG^CGCC
NspV 1702 TTCGAA TT^CGAA
PshAI 1516 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1111 CAGCTG CAG^CTG
RcaI 2046 TCATGA T^CATGA
SacI 502 GAGCTC GAGCT^C
SalI 1824 GTCGAC G^TCGAC
SfoI 3757 GGCGCC GGC^GCC
SfuI 1702 TTCGAA TT^CGAA
SnaBI 1477 TACGTA TAC^GTA
SwaI 4453 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 904 GTCTAC GT^[AC][GT]AC
1824 GTCGAC
AflIII 908 ACACGT A^C[GA][CT]GT
3562 ACATGT
Alw44I 1867 GTGCAC G^TGCAC
3715 GTGCAC
ApaLI 1867 GTGCAC G^TGCAC
3715 GTGCAC
AspI 1092 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCTCGTC
BamHI 3276 GGATCC G^GATCC
3455 GGATCC
BbrPI 3058 CACGTG CAC^GTG
4029 CACGTG
BbsI 1084 GAAGAC GAAGAC(2/6)
2232 GAAGAC
BciVI 1031 GGATAC GTATCC(6/5)
3393 GTATCC
BlpI 634 GCTCAGC GC^T[ACGT]AGC
2651 GCTCAGC
Bpu1102I 634 GCTCAGC GC^T[ACGT]AGC
2651 GCTCAGC
BpuAI 1084 GAAGAC GAAGAC(2/6)
2232 GAAGAC
BsaI 1888 GAGACC GGTCTC(1/5)
2487 GAGACC
BsiEI 1493 CGGCCG CG[GA][CT]^CG
1521 CGGTCG
BsmI 304 GAATGC GAATGC(1/-1)
939 GAATGC
Bsp1407I 932 TGTACA T^GTACA
4214 TGTACA
BspMI 809 GCAGGT ACCTGC(4/8)
2748 GCAGGT
BsrGI 932 TGTACA T^GTACA
4214 TGTACA
CelII 634 GCTCAGC GC^T[ACGT]AGC
2651 GCTCAGC
DrdI 1458 GACGACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3615 GACGTTGGCGTC
EarI 3901 CTCTTC CTCTTC(1/4)
2374 GAAGAG
Ksp632I 3901 CTCTTC CTCTTC(1/4)
2374 GAAGAG
KspI 334 CCGCGG CCGC^GG
3250 CCGCGG
NaeI 1495 GCCGGC GCC^GGC
2700 GCCGGC
NcoI 1862 CCATGG C^CATGG
2916 CCATGG
NgoMIV 1495 GCCGGC G^CCGGC
2700 GCCGGC
PflFI 1092 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCTCGTC
PmaCI 3058 CACGTG CAC^GTG
4029 CACGTG
PmlI 3058 CACGTG CAC^GTG
4029 CACGTG
SacII 334 CCGCGG CCGC^GG
3250 CCGCGG
SmaI 347 CCCGGG CCC^GGG
3131 CCCGGG
SmlI 835 CTCAAG C^T[CT][GA]AG
1417 CTCAAG
SspBI 932 TGTACA T^GTACA
4214 TGTACA
TfiI 3351 GATTC G^A[AT]TC
3918 GATTC
Tth111I 1092 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCTCGTC
XmaCI 347 CCCGGG C^CCGGG
3131 CCCGGG
XmaI 347 CCCGGG C^CCGGG
3131 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI