##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI Asp718I
AspEI AviII BalI BbeI BbvCI
BfrI BglI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BstAPI
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRV FseI FspI Hin1I
KasI KpnI MluI MluNI MroI
MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 823 ACATGT A^C[GA][CT]GT
Aor51HI 2037 AGCGCT AGC^GCT
Asp700I 2165 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 299 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2573 CCCGAG C^[CT]CG[GA]G
AvrII 2470 CCTAGG C^CTAGG
BamHI 2256 GGATCC G^GATCC
BanI 3856 GGCACC G^G[CT][GA]CC
BanII 947 GGGCTC G[GA]GC[CT]^C
BbrPI 877 CACGTG CAC^GTG
BcgI 1625 CGAACACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2314 AGATCT A^GATCT
BlnI 2470 CCTAGG C^CTAGG
BseRI 2147 GAGGAG GAGGAG(10/8)
BsoBI 2573 CCCGAG C^[CT]CG[GA]G
BspHI 2173 TCATGA T^CATGA
BspLU11I 823 ACATGT A^CATGT
BssSI 2144 CACGAG CACGAG(-5/-1)
Bst1107I 2210 GTATAC GTA^TAC
BstBI 1755 TTCGAA TT^CGAA
BstEII 1877 GGTAACC G^GT[ACGT]ACC
BstPI 1877 GGTAACC G^GT[ACGT]ACC
BstXI 2112 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2210 GTATAC GTA^TAC
CfoI 2038 GCGC GCG^C
Eco47III 2037 AGCGCT AGC^GCT
EcoNI 1400 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1877 GGTAACC G^GT[ACGT]ACC
FauI 945 GCGGG CCCGC(4/6)
HaeII 2037 AGCGCT [GA]GCGC^[CT]
HhaI 2038 GCGC GCG^C
HinP1I 2038 GCGC G^CGC
HpaI 161 GTTAAC GTT^AAC
KspI 943 CCGCGG CCGC^GG
NspV 1755 TTCGAA TT^CGAA
PflFI 299 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1868 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 877 CACGTG CAC^GTG
PmlI 877 CACGTG CAC^GTG
PstI 1233 CTGCAG CTGCA^G
RcaI 2173 TCATGA T^CATGA
SacII 943 CCGCGG CCGC^GG
ScaI 811 AGTACT AGT^ACT
SfuI 1755 TTCGAA TT^CGAA
StuI 73 AGGCCT AGG^CCT
Tth111I 299 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1868 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2165 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 944 CGCG CG^CG
2522 CGCG
AseI 1499 ATTAAT AT^TAAT
3158 ATTAAT
AsnI 1499 ATTAAT AT^TAAT
3158 ATTAAT
AspHI 349 GAGCAC G[AT]GC[AT]^C
2303 GAGCAC
BciVI 1843 GGATAC GTATCC(6/5)
1858 GGATAC
BcnI 52 CCGGG CC^[GC]GG
129 CCCGG
BsaAI 877 CACGTG [CT]AC^GT[GA]
1170 CACGTA
BsaBI 916 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1021 GATTGGGATC
BsiHKAI 349 GAGCAC G[AT]GC[AT]^C
2303 GAGCAC
Bsp1407I 85 TGTACA T^GTACA
3278 TGTACA
BspMI 2976 ACCTGC ACCTGC(4/8)
3859 ACCTGC
BsrFI 2419 ACCGGC [GA]^CCGG[CT]
2593 ACCGGC
BsrGI 85 TGTACA T^GTACA
3278 TGTACA
BstUI 944 CGCG CG^CG
2522 CGCG
BstYI 2256 GGATCC [GA]^GATC[CT]
2314 AGATCT
Cfr10I 2419 ACCGGC [GA]^CCGG[CT]
2593 ACCGGC
EcoRI 3109 GAATTC G^AATTC
3178 GAATTC
EcoT22I 192 ATGCAT ATGCA^T
3200 ATGCAT
HgaI 1701 GACGC GACGC(5/10)
1805 GCGTC
HindIII 183 AAGCTT A^AGCTT
537 AAGCTT
MamI 916 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1021 GATTGGGATC
MfeI 1047 CAATTG C^AATTG
2999 CAATTG
MflI 2256 GGATCC [GA]^GATC[CT]
2314 AGATCT
MunI 1047 CAATTG C^AATTG
2999 CAATTG
MvnI 944 CGCG CG^CG
2522 CGCG
NciI 52 CCGGG CC^[GC]GG
129 CCCGG
NsiI 192 ATGCAT ATGCA^T
3200 ATGCAT
PshBI 1499 ATTAAT AT^TAAT
3158 ATTAAT
SapI 1012 GCTCTTC GCTCTTC(1/4)
1451 GCTCTTC
SpeI 2609 ACTAGT A^CTAGT
3778 ACTAGT
SphI 2352 GCATGC GCATG^C
2460 GCATGC
SspBI 85 TGTACA T^GTACA
3278 TGTACA
XcmI 57 CCATGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
516 CCAGTATGAAGTTGG
XhoII 2256 GGATCC [GA]^GATC[CT]
2314 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI