##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AspI BamHI BbeI BbvCI
BcgI BlpI Bpu1102I BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
KasI KpnI KspI MluI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2437 GTAGAC GT^[AC][GT]AC
AccIII 3052 TCCGGA T^CCGGA
AcyI 3208 GACGCC G[GA]^CG[CT]C
AocI 1836 CCTGAGG CC^T[ACGT]AGG
Aor51HI 53 AGCGCT AGC^GCT
ApaI 1336 GGGCCC GGGCC^C
AvaI 342 CTCGAG C^[CT]CG[GA]G
AviII 3396 TGCGCA TGC^GCA
BglI 1456 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1996 AGATCT A^GATCT
BsaHI 3208 GACGCC G[GA]^CG[CT]C
BsaWI 3052 TCCGGA [AT]^CCGG[AT]
BseAI 3052 TCCGGA T^CCGGA
BsoBI 342 CTCGAG C^[CT]CG[GA]G
BspEI 3052 TCCGGA T^CCGGA
BsrBI 3540 CCGCTC CCGCTC(-3/-3)
BssSI 3353 CTCGTG CACGAG(-5/-1)
Bsu36I 1836 CCTGAGG CC^T[ACGT]AGG
DraII 3308 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eco47III 53 AGCGCT AGC^GCT
Eco81I 1836 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3308 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1902 GAATTC G^AATTC
FspI 3396 TGCGCA TGC^GCA
HaeII 53 AGCGCT [GA]GCGC^[CT]
HgaI 3208 GACGC GACGC(5/10)
Hin1I 3208 GACGCC G[GA]^CG[CT]C
HindIII 431 AAGCTT A^AGCTT
HpaI 762 GTTAAC GTT^AAC
MfeI 2866 CAATTG C^AATTG
MroI 3052 TCCGGA T^CCGGA
MunI 2866 CAATTG C^AATTG
NdeI 2191 CATATG CA^TATG
NheI 4042 GCTAGC G^CTAGC
PaeR7I 342 CTCGAG C^TCGAG
PpuMI 3308 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3432 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 921 CAGCTG CAG^CTG
SacI 2090 GAGCTC GAGCT^C
SapI 4128 GAAGAGC GCTCTTC(1/4)
SexAI 3344 ACCTGGT A^CC[AT]GGT
SpeI 2981 ACTAGT A^CTAGT
SphI 1675 GCATGC GCATG^C
StuI 2600 AGGCCT AGG^CCT
XbaI 3158 TCTAGA T^CTAGA
XhoI 342 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1701 GACAGTCGGTC
Alw44I 2640 GTGCAC G^TGCAC
4227 GTGCAC
ApaLI 2640 GTGCAC G^TGCAC
4227 GTGCAC
Asp700I 863 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1197 GAAAATATTC
AspEI 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1701 GACAGTCGGTC
AvrII 2532 CCTAGG C^CTAGG
3570 CCTAGG
BalI 1613 TGGCCA TGG^CCA
2346 TGGCCA
BbrPI 1069 CACGTG CAC^GTG
1078 CACGTG
BbsI 1584 GAAGAC GAAGAC(2/6)
3439 GTCTTC
BciVI 320 GTATCC GTATCC(6/5)
3138 GTATCC
BlnI 2532 CCTAGG C^CTAGG
3570 CCTAGG
BpuAI 1584 GAAGAC GAAGAC(2/6)
3439 GTCTTC
BsaAI 1069 CACGTG [CT]AC^GT[GA]
1078 CACGTG
BsaBI 1991 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2685 GATTCTCATC
BsaI 2184 GAGACC GGTCTC(1/5)
2110 GGTCTC
BsrFI 3243 ACCGGC [GA]^CCGG[CT]
3591 GCCGGT
BssHII 71 GCGCGC G^CGCGC
73 GCGCGC
BstXI 892 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1066 CCACACGTGTGG
Cfr10I 3243 ACCGGC [GA]^CCGG[CT]
3591 GCCGGT
Eam1105I 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1701 GACAGTCGGTC
EcoNI 2791 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3545 CCTGTAGAAGG
FauI 13 CCCGC CCCGC(4/6)
1339 CCCGC
MamI 1991 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2685 GATTCTCATC
MluNI 1613 TGGCCA TGG^CCA
2346 TGGCCA
MscI 1613 TGGCCA TGG^CCA
2346 TGGCCA
NcoI 1977 CCATGG C^CATGG
3733 CCATGG
PmaCI 1069 CACGTG CAC^GTG
1078 CACGTG
PmlI 1069 CACGTG CAC^GTG
1078 CACGTG
XmnI 863 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1197 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaII BanI
BanII BbvI BclI BcnI BfaI
BfrI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII