##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccI AgeI AhdI
Aor51HI AseI AsnI Asp700I Asp718I
AspEI BbeI BbrPI BcgI BclI
BsaBI BsiWI Bsp1407I BspDI BsrGI
Bst1107I BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRI EcoRV FbaI HpaI
KasI KpnI MamI MluI NarI
NcoI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SalI ScaI
SfiI SfoI SgrAI SpeI SspBI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 4405 AACGTT AA^CGTT
ApaI 4157 GGGCCC GGGCC^C
AscI 3424 GGCGCGCC GG^CGCGCC
AspI 474 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4319 CCTAGG C^CTAGG
BbvCI 1653 CCTCAGC CCTCAGC(-5/-2)
BglII 3493 AGATCT A^GATCT
BlnI 4319 CCTAGG C^CTAGG
BsaAI 4565 TACGTA [CT]AC^GT[GA]
BsiEI 2806 CGGCCG CG[GA][CT]^CG
BssHII 3425 GCGCGC G^CGCGC
BssSI 3848 CACGAG CACGAG(-5/-1)
BstAPI 3073 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3725 TTCGAA TT^CGAA
DraI 2539 TTTAAA TTT^AAA
EagI 2806 CGGCCG C^GGCCG
EclXI 2806 CGGCCG C^GGCCG
Eco52I 2806 CGGCCG C^GGCCG
KspI 4141 CCGCGG CCGC^GG
MfeI 4444 CAATTG C^AATTG
MunI 4444 CAATTG C^AATTG
NdeI 2091 CATATG CA^TATG
NspV 3725 TTCGAA TT^CGAA
PflFI 474 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3984 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4405 AACGTT AA^CGTT
SacII 4141 CCGCGG CCGC^GG
SfuI 3725 TTCGAA TT^CGAA
SnaBI 4565 TACGTA TAC^GTA
SphI 4294 GCATGC GCATG^C
StuI 3732 AGGCCT AGG^CCT
Tth111I 474 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 564 CCAAGGATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2219 TCCGGA T^CCGGA
2652 TCCGGA
AcyI 2063 GACGCC G[GA]^CG[CT]C
4197 GACGCC
AflII 175 CTTAAG C^TTAAG
2384 CTTAAG
Alw44I 1818 GTGCAC G^TGCAC
2700 GTGCAC
AocI 1293 CCTTAGG CC^T[ACGT]AGG
4250 CCTGAGG
ApaLI 1818 GTGCAC G^TGCAC
2700 GTGCAC
AviII 3130 TGCGCA TGC^GCA
4291 TGCGCA
BalI 1168 TGGCCA TGG^CCA
3180 TGGCCA
BamHI 1126 GGATCC G^GATCC
1875 GGATCC
BanI 1534 GGTGCC G^G[CT][GA]CC
4269 GGTGCC
BciVI 2606 GGATAC GTATCC(6/5)
2993 GGATAC
BfrI 175 CTTAAG C^TTAAG
2384 CTTAAG
BglI 2627 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3056 GCCCGTGAGGC
BlpI 3065 GCTGAGC GC^T[ACGT]AGC
3457 GCTGAGC
Bpu1102I 3065 GCTGAGC GC^T[ACGT]AGC
3457 GCTGAGC
BsaHI 2063 GACGCC G[GA]^CG[CT]C
4197 GACGCC
BseAI 2219 TCCGGA T^CCGGA
2652 TCCGGA
BsmBI 2615 CGTCTC CGTCTC(1/5)
2665 CGTCTC
BspEI 2219 TCCGGA T^CCGGA
2652 TCCGGA
BspHI 229 TCATGA T^CATGA
3607 TCATGA
BsrFI 2718 GCCGGC [GA]^CCGG[CT]
2804 GCCGGC
BstXI 1192 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3114 CCAGACAGCTGG
Bsu36I 1293 CCTTAGG CC^T[ACGT]AGG
4250 CCTGAGG
CelII 3065 GCTGAGC GC^T[ACGT]AGC
3457 GCTGAGC
Cfr10I 2718 GCCGGC [GA]^CCGG[CT]
2804 GCCGGC
DrdI 752 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3578 GACCACAAAGTC
DsaI 3177 CCGTGG C^C[GA][CT]GG
4141 CCGCGG
Eco81I 1293 CCTTAGG CC^T[ACGT]AGG
4250 CCTGAGG
EcoT22I 4313 ATGCAT ATGCA^T
4683 ATGCAT
FseI 2717 GGCCGGCC GGCCGG^CC
2803 GGCCGGCC
FspI 3130 TGCGCA TGC^GCA
4291 TGCGCA
HaeII 2471 AGCGCC [GA]GCGC^[CT]
2866 AGCGCC
Hin1I 2063 GACGCC G[GA]^CG[CT]C
4197 GACGCC
HindIII 2547 AAGCTT A^AGCTT
2978 AAGCTT
MluNI 1168 TGGCCA TGG^CCA
3180 TGGCCA
MroI 2219 TCCGGA T^CCGGA
2652 TCCGGA
MscI 1168 TGGCCA TGG^CCA
3180 TGGCCA
NaeI 2718 GCCGGC GCC^GGC
2804 GCCGGC
NgoMIV 2718 GCCGGC G^CCGGC
2804 GCCGGC
NsiI 4313 ATGCAT ATGCA^T
4683 ATGCAT
RcaI 229 TCATGA T^CATGA
3607 TCATGA
SapI 416 GAAGAGC GCTCTTC(1/4)
4067 GAAGAGC
SbfI 3430 CCTGCAGG CCTGCA^GG
3562 CCTGCAGG
SexAI 1381 ACCAGGT A^CC[AT]GGT
3669 ACCAGGT
SmaI 693 CCCGGG CCC^GGG
2248 CCCGGG
Sse8387I 3430 CCTGCAGG CCTGCA^GG
3562 CCTGCAGG
SspI 1679 AATATT AAT^ATT
4708 AATATT
XbaI 517 TCTAGA T^CTAGA
1624 TCTAGA
XmaCI 693 CCCGGG C^CCGGG
2248 CCCGGG
XmaI 693 CCCGGG C^CCGGG
2248 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII