##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BsrFI BssHII BssSI
BstAPI BstBI BstUI CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
Eam1105I Eco47III EcoO109I FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NspV PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I Tth111I Van91I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 24 GACGCC G[GA]^CG[CT]C
AflIII 227 ACATGT A^C[GA][CT]GT
Alw44I 3021 GTGCAC G^TGCAC
AocI 1497 CCTGAGG CC^T[ACGT]AGG
ApaLI 3021 GTGCAC G^TGCAC
AspHI 3021 GTGCAC G[AT]GC[AT]^C
AvaI 1557 CTCGAG C^[CT]CG[GA]G
AvaII 281 GGACC G^G[AT]CC
BciVI 583 GTATCC GTATCC(6/5)
BclI 3909 TGATCA T^GATCA
BcnI 610 CCCGG CC^[GC]GG
BglII 1214 AGATCT A^GATCT
BmyI 3021 GTGCAC G[AGT]GC[ACT]^C
BsaHI 24 GACGCC G[GA]^CG[CT]C
BsgI 2073 CTGCAC GTGCAG(16/14)
BsiEI 52 CGGCCG CG[GA][CT]^CG
BsiHKAI 3021 GTGCAC G[AT]GC[AT]^C
BsmI 1059 GCATTC GAATGC(1/-1)
BsoBI 1557 CTCGAG C^[CT]CG[GA]G
Bsp1286I 3021 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 227 ACATGT A^CATGT
BspMI 1046 GCAGGT ACCTGC(4/8)
BsrBI 102 GAGCGG CCGCTC(-3/-3)
BstEII 425 GGTCACC G^GT[ACGT]ACC
BstPI 425 GGTCACC G^GT[ACGT]ACC
BstXI 2123 CCATGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1214 AGATCT [GA]^GATC[CT]
Bsu36I 1497 CCTGAGG CC^T[ACGT]AGG
CfoI 69 GCGC GCG^C
EaeI 52 CGGCCG [CT]^GGCC[GA]
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
Eco81I 1497 CCTGAGG CC^T[ACGT]AGG
EcoO65I 425 GGTCACC G^GT[ACGT]ACC
EcoRI 626 GAATTC G^AATTC
EcoT14I 2443 CCAAGG C^C[AT][AT]GG
EcoT22I 3046 ATGCAT ATGCA^T
FauI 65 GCGGG CCCGC(4/6)
FbaI 3909 TGATCA T^GATCA
HapII 611 CCGG C^CGG
HhaI 69 GCGC GCG^C
Hin1I 24 GACGCC G[GA]^CG[CT]C
HinP1I 69 GCGC G^CGC
HpaII 611 CCGG C^CGG
MflI 1214 AGATCT [GA]^GATC[CT]
MspI 611 CCGG C^CGG
NciI 610 CCCGG CC^[GC]GG
NdeI 3741 CATATG CA^TATG
NsiI 3046 ATGCAT ATGCA^T
PacI 3639 TTAATTAA TTAAT^TAA
PaeR7I 1557 CTCGAG C^TCGAG
PleI 5 GAGTC GAGTC(4/5)
SnaBI 4057 TACGTA TAC^GTA
SpeI 3246 ACTAGT A^CTAGT
StuI 1072 AGGCCT AGG^CCT
StyI 2443 CCAAGG C^C[AT][AT]GG
SwaI 3303 ATTTAAAT ATTT^AAAT
XhoI 1557 CTCGAG C^TCGAG
XhoII 1214 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 850 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2004 CAGATACTG
BglI 12 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3650 GCCAACTTGGC
BpmI 190 CTCCAG CTGGAG(16/14)
764 CTCCAG
BsaAI 580 CACGTA [CT]AC^GT[GA]
4057 TACGTA
Bst1107I 2777 GTATAC GTA^TAC
2789 GTATAC
BstZ17I 2777 GTATAC GTA^TAC
2789 GTATAC
Cfr13I 281 GGACC G^G[ACGT]CC
3041 GGCCC
DsaI 587 CCGTGG C^C[GA][CT]GG
3711 CCGTGG
EcoNI 793 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2745 CCTGGGGAAGG
EcoRV 1755 GATATC GAT^ATC
3879 GATATC
HgaI 24 GACGC GACGC(5/10)
700 GACGC
PstI 1816 CTGCAG CTGCA^G
1948 CTGCAG
PvuII 619 CAGCTG CAG^CTG
3215 CAGCTG
Sau96I 281 GGACC G^G[ACGT]CC
3041 GGCCC
SmlI 678 CTTGAG C^T[CT][GA]AG
1557 CTCGAG
SphI 2116 GCATGC GCATG^C
2189 GCATGC
XbaI 815 TCTAGA T^CTAGA
3606 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
BbsI BbvI BfaI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NspI
PshBI RsaI Sau3AI ScaI ScrFI
SfaNI SfcI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI