##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI FspI KasI
KpnI KspI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 2175 AACGTT AA^CGTT
AcyI 916 GACGCC G[GA]^CG[CT]C
AflII 3296 CTTAAG C^TTAAG
ApaI 632 GGGCCC GGGCC^C
AvaI 635 CCCGGG C^[CT]CG[GA]G
BalI 1503 TGGCCA TGG^CCA
BanI 2815 GGTGCC G^G[CT][GA]CC
BbvCI 2921 CCTCAGC CCTCAGC(-5/-2)
BciVI 1565 GGATAC GTATCC(6/5)
BclI 1530 TGATCA T^GATCA
BfrI 3296 CTTAAG C^TTAAG
BglI 652 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1205 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 916 GACGCC G[GA]^CG[CT]C
BsmBI 1111 GAGACG CGTCTC(1/5)
BsoBI 635 CCCGGG C^[CT]CG[GA]G
BspHI 842 TCATGA T^CATGA
BsrDI 2778 CATTGC GCAATG(2/0)
DraIII 2181 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1460 CCGTGG C^C[GA][CT]GG
EcoNI 1556 CCTTGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3568 ATGCAT ATGCA^T
FbaI 1530 TGATCA T^GATCA
HaeII 88 GGCGCT [GA]GCGC^[CT]
Hin1I 916 GACGCC G[GA]^CG[CT]C
HindIII 2349 AAGCTT A^AGCTT
MamI 1205 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1117 CAATTG C^AATTG
MluNI 1503 TGGCCA TGG^CCA
MscI 1503 TGGCCA TGG^CCA
MspA1I 2924 CAGCTG C[AC]G^C[GT]G
MunI 1117 CAATTG C^AATTG
NheI 2944 GCTAGC G^CTAGC
NsiI 3568 ATGCAT ATGCA^T
PmeI 1999 GTTTAAAC GTTT^AAAC
Psp1406I 2175 AACGTT AA^CGTT
PstI 691 CTGCAG CTGCA^G
PvuII 2924 CAGCTG CAG^CTG
RcaI 842 TCATGA T^CATGA
SapI 3669 GCTCTTC GCTCTTC(1/4)
SmaI 635 CCCGGG CCC^GGG
SspI 3099 AATATT AAT^ATT
XbaI 2607 TCTAGA T^CTAGA
XcmI 2571 CCATCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 635 CCCGGG C^CCGGG
XmaI 635 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 612 CGCG CG^CG
671 CGCG
AlwNI 86 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGGAACTG
BseRI 3737 CTCCTC GAGGAG(10/8)
499 GAGGAG
BstAPI 708 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3546 GCATTTAATGC
BstUI 612 CGCG CG^CG
671 CGCG
CfoI 89 GCGC GCG^C
672 GCGC
EaeI 1503 TGGCCA [CT]^GGCC[GA]
1667 TGGCCG
EcoT14I 1199 CCTTGG C^C[AT][AT]GG
1664 CCTTGG
FauI 2551 CCCGC CCCGC(4/6)
202 GCGGG
HhaI 89 GCGC GCG^C
672 GCGC
HinP1I 89 GCGC G^CGC
672 GCGC
MvnI 612 CGCG CG^CG
671 CGCG
PflMI 244 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2432 CCAAGTTTTGG
PpuMI 2427 AGGACCC [GA]G^G[AT]CC[CT]
3471 AGGACCT
SmlI 448 CTCAAG C^T[CT][GA]AG
3296 CTTAAG
SphI 661 GCATGC GCATG^C
1004 GCATGC
StyI 1199 CCTTGG C^C[AT][AT]GG
1664 CCTTGG
Van91I 244 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2432 CCAAGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI